Avicularin, an orally active flavonoid, attenuates activities of NF-κB (p65), COX-2, and PPAR-γ, exhibiting anti-inflammatory, anti-infectious, anti-allergic, antioxidant, hepatoprotective, and anti-tumor properties[1][3].
体外研究
Avicularin, at concentrations of 10-300 μM and a one-hour exposure, diminishes NO and PGE2 production in LPS-stimulated RAW 264.7 cells[1].
Avicularin/扁蓄苷 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Huh7 cells
100, 50, 25 µg/ml
12, 24, 36, 48 hours
Avicularin significantly inhibited Huh7 cell proliferation in a concentration-dependent manner and suppressed cell migration and invasion.
Mol Med Rep. 2019 Jun;19(6):5417-5423.
Α-glucosidase
17.05 ± 0.75 μg/mL (IC50)
15 minutes
To evaluate the inhibitory activity of Avicularin against α-glucosidase, results showed an IC50 value of 17.05 ± 0.75 μg/mL, indicating significant inhibitory effect.
Saudi Pharm J. 2023 Aug;31(8):101677.
RAW264.7 macrophages
0, 10, 30, 100, 300, 600 µM
24 hours
To evaluate the effect of AL on the viability of RAW264.7 macrophages, results showed that AL at 10-300 μM did not significantly affect cell viability.
J Agric Food Chem. 2023 Jan 11;71(1):411-420.
RAW 264.7 cells
30, 100 and 300 µM
24 hours
To investigate the inhibitory effect of AL on LPS-induced inflammatory response, results showed that AL significantly suppressed the expression of iNOS, COX-2, IL-6, TNF-α and IL-1β.
J Cell Mol Med. 2023 Nov;27(21):3326-3338.
Bone marrow-derived macrophages (BMMs)
0, 10, 30, 100, 300 µM
5 days
To evaluate the effect of AL on the differentiation of BMMs into osteoclasts, results showed that AL significantly inhibited osteoclast formation and activity.
J Agric Food Chem. 2023 Jan 11;71(1):411-420.
HepG2 cells
10, 20 and 40 µM
6 hours
To investigate the inhibitory effect of AL on D-GalN-induced ferroptosis, results showed that AL significantly reduced MDA, ROS and iron levels, increased GSH content, and upregulated the expression of GPX4 and xCT.
J Cell Mol Med. 2023 Nov;27(21):3326-3338.
MDCK cells
300 to 3.7 µg/mL
72 hours
To evaluate the cytotoxicity of the extracts and determine their 50% cytotoxic concentration (CC50).
Foods. 2024 Dec 11;13(24):4008.
Avicularin/扁蓄苷 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6 mice
Ovariectomy (OVX)-induced osteoporosis model
Intraperitoneal injection
1.25 mg/kg, 5 mg/kg
Every 2 days for 8 weeks
To evaluate the protective effect of AL on OVX-induced osteoporosis in mice, results showed that AL significantly increased bone mineral density and reduced the expression of bone resorption marker CTX-1.
J Agric Food Chem. 2023 Jan 11;71(1):411-420.
ICR mice
Pb-induced nerve damage model
Intragastric administration
25 mg/kg and 50 mg/kg
Once daily for 3 months
To evaluate the protective effects of AVL against Pb-induced neurotoxicity, results showed that AVL significantly ameliorated Pb-induced memory impairment, neuroinflammation, ferroptosis, and oxidative stress.
Antioxidants (Basel). 2024 Aug 22;13(8):1024
ICR mice
Lead-induced liver injury model
Intragastric administration
25 mg/kg and 50 mg/kg
Once daily for 3 months
Evaluate the protective effects of Avi against Pb-induced hepatotoxicity, results showed that Avi significantly alleviated Pb-induced hepatic inflammation, endoplasmic reticulum stress (ERS), and glucose metabolism disorder
Nutrients. 2022 Nov 13;14(22):4806
Male C57BL/6 mice
LPS/D-GalN-induced acute liver failure model
Intragastric administration
50 mg/kg
Once daily for 3 days
To investigate the protective effect of AL on LPS/D-GalN-induced acute liver failure, results showed that AL significantly alleviated hepatic pathological injury, reduced ALT and AST levels, and inhibited inflammatory response and ferroptosis.
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