There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Tolfenamic Acid is a nonsteroidal antiinflammatory agent, selectively inhibits COX-2, with an IC50 of 13.49 μM (3.53 μg/mL) in LPS-treated (COX-2) canine DH82 monocyte/macrophage cells, but shows no effect on COX-1[3]. Tolfenamic Acid also acts as a potent Sp protein inhibitor. Tolfenamic acid (TA) inhibits cancer cell proliferation and tumor growth through the degradation of Sp1, Sp3, and Sp4. Tolfenamic Acid (50 mg/kg 3 times/wk, p.o.) inhibits tumor formation and tumor incidence in N-nitrosomethylbenzylamine (NMBA)-induced esophageal tumor model. Tolfenamic Acid also causes decreases in tumor multiplicity and tumor volume in rats treated with NMBA[4]. Tolfenamic Acid (50 μM) significantly affects gene expression in L3.6pl cells, and downregulates CENPF, KIF20A, LMNB1, MYB, SKP2, CCNE2, and DDIT3[5]. TA has shown excellent in vitro antibacterial activity against certain ATCC strains of bacteria when complexed with bismuth(III) [6].
Tolfenamic Acid/托灭酸 细胞实验
Cell Line
Concentration
Treated Time
Description
References
FG cells
2.5, 5.0, 10.0, 20.0, 40.0 μM TA
24-48 hours
To evaluate the cytotoxicity of TA on FG cells, results showed dose-dependent inhibition of cell proliferation
Cancer Res. 2010 Feb 1;70(3):1111-9.
PANC-1 cells
0.05 μM MIT + 5 μM TA
12 hours
To evaluate the effect of combined MIT and TA on Sp1 protein expression, results showed significant downregulation of Sp1 protein expression
Cancer Res. 2010 Feb 1;70(3):1111-9.
PANC-1 cells
5, 10, 20 μM
24 hours
To evaluate the effect of TA on Sp1 protein expression, results showed dose-dependent downregulation of Sp1 protein expression
Cancer Res. 2010 Feb 1;70(3):1111-9.
SKBR3 cells
25-100 µM
6 days
Tolfenamic acid inhibited the proliferation of SKBR3 cells with an IC50 value of 52.5 µM.
Mol Cancer Ther. 2009 May;8(5):1207-17.
BT474 cells
25-100 µM
6 days
Tolfenamic acid inhibited the proliferation of BT474 cells with an IC50 value of 41.5 µM.
Mol Cancer Ther. 2009 May;8(5):1207-17.
RD cells
100 μM
24 or 48 hours
Inhibited cell proliferation and migration, induced apoptosis
Int J Cancer. 2013 Feb 15;132(4):795-806.
RH30 cells
75 μM
24 or 48 hours
Inhibited cell proliferation and migration, induced apoptosis
Int J Cancer. 2013 Feb 15;132(4):795-806.
Schistosoma mansoni adult worms
50 μM
72 hours
Evaluation of the schistosomicidal effect of Tolfenamic Acid on adult worms, LC50 of 20.6 μM.
EBioMedicine. 2019 May;43:370-379.
SH-SY5Y cells
25 µM
72 hours
To evaluate the inhibitory effects of TA and its analogs TN3 and TN7 on SP1-DNA binding. Results showed that TA, TN3, and TN7 all reduced SP1-DNA binding.
Int J Mol Sci. 2023 Oct 16;24(20):15216.
SH-SY5Y neuroblastoma cells
25 μM and 50 μM
48 hours and 96 hours
To investigate the effects of tolfenamic acid on Pb-induced SP1, APP, and Aβ levels. Results showed that tolfenamic acid significantly reduced Pb-induced SP1, APP, and Aβ levels.
Neuropharmacology. 2014 Apr;79:596-602.
H9C2 myoblasts
50 μM
6 hours
Inhibition of SP1 to mitigate Aβ-induced cell death and cardiomyocyte contractile dysfunction
Acta Pharmacol Sin. 2022 Jan;43(1):39-49.
Tolfenamic Acid/托灭酸 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Athymic nude mice
BT474 cell mammary fat pad xenograft model
Oral gavage
25 mg/kg/d
Once daily for 27 days
Tolfenamic acid significantly inhibited the growth of BT474 cell xenografts and reduced tumor weight.
Mol Cancer Ther. 2009 May;8(5):1207-17.
Athymic nude mice
RH30 cell xenograft model
Oral gavage
50 mg/kg/dose
Every second day for 20 days
Inhibited tumor growth, downregulated Sp1, Sp3, Sp4 and Sp-regulated genes
Int J Cancer. 2013 Feb 15;132(4):795-806.
APP YAC transgenic mice
Alzheimer's disease model
Oral gavage
5 mg/kg and 50 mg/kg
Once daily for 34 days
To investigate the effects of tolfenamic acid on BACE1 gene expression and enzyme activity. Results showed that tolfenamic acid significantly reduced BACE1 gene expression and enzyme activity.
Neuropharmacology. 2014 Apr;79:596-602.
Female Swiss mice
Schistosoma mansoni infection model
Oral
400 mg/kg single dose
Single dose
Evaluation of the schistosomicidal effect of Tolfenamic Acid on adult worms in mice, worm burden reduction of 62.4%.
EBioMedicine. 2019 May;43:370-379.
Transgenic mice
Alzheimer's disease model
Oral gavage
5 or 50 mg/kg
Once daily for 34 days
Tolfenamic acid lowers tau mRNA and protein, as well as the levels of its phosphorylated form and CDK5.
J Neurochem. 2015 Apr;133(2):266-72
Mice
AD transgenic mouse model
Oral gavage
5 and 50 mg/kg/day
Daily administration for 34 days
To evaluate the cognitive improvement effects of tolfenamic acid, results showed that 50 mg/kg/day dose significantly improved long-term and working memory deficits.
Neurobiol Aging. 2013 Oct;34(10):2421-30
Nude mice
PANC-1 xenograft model
Oral gavage
10 mg/kg
Twice a week for 45 days
To evaluate the effect of TA alone on tumor growth, results showed marginal antitumor activity at low doses
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