Sodium 2-Hydroxybenzoate (Sodium Salicylate) inhibits cyclo-oxygenase-2 (COX-2) activity independently of transcription factor (NF-κB) activation[3]. Sodium salicylate and aspirin also inhibited NF-kappa B-dependent transcription from the Ig kappa enhancer and the human immunodeficiency virus (HIV) long terminal repeat (LTR) in transfected T cells[4]. Sodium salicylate effectively inhibited TNF-alpha-induced upregulation of NF-kappaB, ICAM-1 expression, in-vitro migration and invasion in human melanoma cells, indicating that non-steroidal anti-inflammatory drugs may be a useful therapeutic approach to oppose inflammation-induced melanoma invasion and metastasis in vivo[5]. PC12 cells were treated with different concentrations of sodium salicylate (1-20 mM). Higher concentrations (10-20 mM) killed PC12 cells in a dose-dependent manner. A decreased NF-kappaB activity after sodium salicylate treatment by electrophoretic mobility shift assay (EMSA)[6]. In the RAW264.7 macrophage cell line, suprapharmacological concentrations of sodium salicylate exert a potent inhibitory effect on LPS-induced cytokine gene induction but appear to accomplish this by interfering with NF-kappaB-independent pathways of activation[7]. Moreover, salicylate significantly improved retinal function, as well as reduced TNFα and SOCS3-induced insulin resistance in all samples[8].
Sodium Salicylate/水杨酸钠 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Mouse diaphragm muscle cells
5 mM
10 minutes
To study the effect of sodium salicylate on the quantal content of endplate potentials (e.p.ps). Results showed that sodium salicylate increased the quantal content of e.p.ps, and this effect was dependent on the concentration of [Ca2+].
Br J Pharmacol. 1989 Aug;97(4):1239-45
Mouse diaphragm muscle cells
10 mM
10-15 minutes
To study the effect of sodium salicylate on spontaneous transmitter release at the mouse neuromuscular junction. Results showed that at 36°C, sodium salicylate increased the frequency of miniature endplate potentials (m.e.p.ps), and this effect was insensitive to [Mg2+] and independent of the presence of [Ca2+].
Br J Pharmacol. 1989 Aug;97(4):1239-45
Inferior colliculus neurons
350 mg/L
2-4 hours
To investigate the effect of sodium salicylate on spontaneous firing of inferior colliculus neurons, results showed that sodium salicylate significantly reduced the spontaneous firing rate in most neurons
iScience. 2024 Apr 9;27(5):109691
Mouse cochlear inner hair cells (IHCs)
10-20 mM
48 hours
To study the damaging effects of high concentrations of sodium salicylate on inner hair cells, found that 10-20 mmol/L salicylate caused significant damage to IHCs.
Neurosci Bull. 2022 Jan;38(1):69-80
Mouse cochlear inner hair cells (IHCs)
1-5 mM
48 hours
To investigate the effects of sodium salicylate on calcium currents and exocytosis in inner hair cells, found that 1-5 mmol/L salicylate did not affect the exocytosis of IHCs but increased the peak amplitude of calcium currents.
Neurosci Bull. 2022 Jan;38(1):69-80
Pseudomonas aeruginosa PAO1 ΔlasR ΔrhlR
10 mM
48 hours
To evaluate the effect of NaSa on biofilm formation of QS-deficient strain, results showed that NaSa had no significant effect on biofilm formation of PAO1 ΔlasR ΔrhlR.
Int J Mol Sci. 2021 Jan 21;22(3):1060
Pseudomonas aeruginosa PAO1 wt
10 mM
48 hours
To evaluate the effect of NaSa on biofilm formation of PAO1 wt, results showed that NaSa significantly reduced biofilm biomass.
Int J Mol Sci. 2021 Jan 21;22(3):1060
Sodium Salicylate/水杨酸钠 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Anesthetized and awake mice
Intraperitoneal injection
250 mg/kg
Single injection
To investigate the effect of sodium salicylate on spontaneous and sound-evoked activity in the inferior colliculus of mice, results showed that sodium salicylate significantly reduced spontaneous firing and improved the detection of amplitude-modulated sounds
iScience. 2024 Apr 9;27(5):109691
Mice
NaSal-induced hyperacusis and anxiety-like behaviors model
Intraperitoneal injection
300 mg/kg
Single injection
To investigate the neural mechanisms underlying NaSal-induced hyperacusis and anxiety-like behaviors
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