Neochlorogenic Acid is a naturally occuring phenolic acid commonly found in apricots with effect on inhibiting the oxidation of low-density lipoprotein. Neochlorogenic acid (NCA) shows a reduction of lipopolysaccharide (LPS)-induced NO production by suppressing iNOS and COX-2 protein expression and production of pro-inflammatory cytokines, such as TNF-α and IL-1β, in BV2 microglia cells. In addition, phosphorylated p38 MAPK and NF-κB p65 are also inhibited by Neochlorogenic acid in activated microglia. iNOS and COX-2 levels are increased in LPS-induced BV2 cells, but this increase is significantly inhibited after treatment with 50 and 100 μM Neochlorogenic acid[3]. Neochlorogenic acid exhibited an IC50 of 20 µM in human gastric carcinoma cells. After 6 weeks of neochlorogenic acid administration to mice, the average tumor volumes and growth for the untreated control group were significantly higher than the treated groups[4]. Neochlorogenic acid showed the anti-photoaging effect through ameliorating UVB-induced collagen degradation, reinforcing the skin barrier[5].
Neochlorogenic acid/新绿原酸 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Rat bone marrow mesenchymal stem cells (BMSCs)
0, 0.4, 0.8, 1.6, 3.2, 6.4 µM
24 hours
Evaluated the cytotoxicity of Ncg on BMSCs, found that 1.6 μM concentration had the strongest proliferative effect.
Biomacromolecules. 2024 Feb 12;25(2):729-740
HepG2 cells
40-120 µM
24 hours
5-CQA alleviates lipid accumulation by downregulating miR-34a, leading to activation of the SIRT1/AMPK pathway
Int J Mol Sci. 2021 Dec 6;22(23):13163
Rat aortic vascular smooth muscle cells (A7r5 cell line)
40, 80, 120 µM
24 hours
Inhibited OH-induced proliferation and migration of A7r5 cells by suppressing FAK/Src, PI3K/Akt, and Ras-related signaling pathways
Nutrients. 2022 Jul 22;14(15):3006
A549 cells
0, 25, 50, 100 µM
24 hours
To investigate the anti-inflammatory effects of nCGA on LPS-stimulated inflammatory response in A549 cells. Results showed that nCGA significantly reduced the production of TNF-α, IL-6, and NO, and suppressed the protein expression of iNOS, COX2, TNF-α, and IL-6. Additionally, nCGA inhibited NF-κB activation and blocked MAPKs signaling pathway phosphorylation.
Molecules. 2020 Mar 18;25(6):1385
Rat bone marrow mesenchymal stem cells (BMSCs)
1.6 µM
7 days
Evaluated the effect of Ncg on chondrogenic differentiation of BMSCs, found that Ncg promoted BMSCs differentiation toward cartilage and secretion of more Aggrecan.
Biomacromolecules. 2024 Feb 12;25(2):729-740
Neochlorogenic acid/新绿原酸 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Db/db mice
High-fat diet-induced diabetic fatty liver model
Gavage
250 mg/kg
Once daily for 12 weeks
To investigate the protective effects of neochlorogenic acid on high-fat diet-induced diabetic fatty liver. Results showed that neochlorogenic acid significantly improved serum lipid profiles, liver fat deposition conditions, steatohepatitis levels, and liver antioxidant activity.
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