Loganin, a major iridoid glycoside obtained from Corni fructus, has been shown to have anti-inflammatory and anti-shock effects.
Loganin/马钱子苷 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Transgenic cell line S10
200 μM
14 days
To investigate the effect of continuous loganin supply on TIA accumulation, results showed that continuous loganin supply significantly increased TIA content but had no significant effect on TDC activity.
Plant Physiol. 1998 Feb 1;116(2):853-7
Transgenic cell line S10
500 μM
3 days or 3 days
To study the effect of loganin on TIA content, results showed that exogenous loganin significantly increased TIA content while depleting tryptamine content.
Plant Physiol. 1998 Feb 1;116(2):853-7
BEnd.3 cells
6.25 µM
24 hours
Evaluate the cytotoxicity of LA-1 and loganin on BEnd.3 cells, results showed LA-1 did not exhibit significant cytotoxicity under the given conditions
Antioxidants (Basel). 2024 Nov 1;13(11):1339
Rat Schwann cell line RSC96
0.1, 1, 10, 25, 50 μM
48 hours
Loganin pretreatment attenuated high glucose-induced RSC96 cell viability loss, ROS generation, NF-κB nuclear translocation, P2X7 purinergic receptor and TXNIP expression, NLRP3 inflammasome activation, IL-1β and IL-18 maturation, and gasdermin D cleavage.
Cells. 2020 Aug 23;9(9):1948
SH-SY5Y cells
10 μM
24 hours
Loganin attenuates high glucose-induced SH-SY5Y cell pyroptosis by inhibiting ROS generation and NLRP3 inflammasome activation, improving cell viability.
Cells. 2021 Oct 8;10(10):2688
Mouse bone marrow monocytes
1, 5, 10 μM
5 days
To investigate the effect of Loganin on the differentiation of mouse bone marrow monocytes. Results showed that Loganin treatment significantly decreased TRAP activity and the number of TRAP-positive multinucleated cells, indicating that Loganin inhibited osteoclast differentiation.
Int J Mol Sci. 2022 Nov 16;23(22):14128
Mouse preosteoblast MC3T3-E1 cells
1, 5, 10 μM
3 days
To investigate the effect of Loganin on the differentiation of mouse preosteoblasts. Results showed that Loganin treatment increased ALP activity and the number of ALP-positive cells, indicating that Loganin promoted osteoblast differentiation.
Int J Mol Sci. 2022 Nov 16;23(22):14128
Adipose-derived stem cells (ADSCs)
2, 5, 10 μM
8 days
Loganin reduced mRNA expression levels of Pparγ, Cebpa, Plin2, Fasn, and Srebp1 and decreased the number of oil Red O-positive cells.
Int J Mol Sci. 2023 Mar 1;24(5):4752
3T3-L1 mouse preadipocytes
2, 5, 10 μM
8 days
Loganin significantly decreased the mRNA expression levels of Pparg, Cebpa, Plin2, Fasn, and Srebp1 in a dose-dependent manner and reduced the number of oil Red O-positive cells.
Int J Mol Sci. 2023 Mar 1;24(5):4752
Loganin/马钱子苷 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Sprague Dawley (SD) rats
STZ-NA-induced diabetic neuropathy model
Intraperitoneal injection
5 mg/kg
Once daily for 4 weeks
Loganin improved pain behaviors (allodynia and hyperalgesia), insulin resistance index (HOMA-IR), and serum levels of superoxide dismutase (SOD), catalase, and glutathione in diabetic neuropathic pain rats. Loganin also reduced the expression of pain-associated channel protein CaV3.2 and calcitonin gene-related peptide (CGRP) in the superficial spinal dorsal horn of PDN rats.
Cells. 2021 Oct 8;10(10):2688
DdY mice
Ovariectomy-induced osteoporosis model
Oral
2, 10, 50 mg/kg/day
Once daily for 12 weeks
To investigate the effect of Loganin on ovariectomy-induced osteoporosis. Results showed that Loganin treatment prevented BMD loss, improved trabecular microstructure, and increased the serum OPG/RANKL ratio, indicating that Loganin has anti-osteoporotic effects.
Int J Mol Sci. 2022 Nov 16;23(22):14128
Sprague-Dawley rats
Chronic constriction injury (CCI) model
Intraperitoneal injection
5 mg/kg/day
Once daily for 7 days
Loganin alleviates CCI-induced neuropathic pain by modulating autophagic flux and reducing neuroinflammation and apoptosis
Int J Mol Sci. 2022 Dec 14;23(24):15873
C57BL/6 mice
Cisplatin-induced acute kidney injury model
Oral
1, 10, and 20 mg/kg
Administered 1 h before cisplatin injection and lasted for 72 hours
Loganin attenuates the severity of cisplatin-induced acute kidney injury by inhibiting ERK1/2 activation
Int J Mol Sci. 2021 Jan 31;22(3):1421
Mice
OVX- and high-fat diet (HFD)-induced obesity models
Oral
2 and 10 mg/kg/day
12 weeks
Loganin prevented weight gain induced by OVX and HFD, reduced hepatic steatosis and adipocyte enlargement, and decreased plasma leptin and insulin levels.
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