GlucoAurantio-obtusin, a natural product isolated and purified from the seeds of Cassia obtusifolia L., possesses hypotensive and hypolipidemic effects.
Aurantio-obtusin/橙黄决明素 细胞实验
Cell Line
Concentration
Treated Time
Description
References
primary mature BAT adipocytes
15 μM
5 days
AO significantly increased the activities of mitochondrial complexes I and IV and decreased ATP levels, suggesting that AO promotes energy expenditure by activating UCP1-mediated mitochondrial respiration uncoupling.
Acta Pharmacol Sin. 2023 Sep;44(9):1826-1840
Primary BAT adipocytes
15 μM
24 h
To study the effect of AO on oxidative stress and mitochondrial function in primary BAT adipocytes, AO reduced oxidative stress and inhibited mPTP opening.
Chin Med. 2025 Mar 25;20(1):41
AML12 cells
15 μM
24 h
To investigate the effect of AO on inflammation in AML12 cells, AO reduced inflammation in AML12 cells by inhibiting mtDNA release from BAT cells.
Chin Med. 2025 Mar 25;20(1):41
HepG2 cells
10-600 µM
48 h
To evaluate the inhibitory effect of AO on liver cancer cell activity, the results showed that AO significantly inhibited the proliferation of SK-Hep1 and HepG2 cells.
Int J Oncol. 2024 Oct;65(4):92
HepG2 cells
25 μM
3 and 5 h
AO effectively promoted autophagy flux in HepG2 cells, as evidenced by a sustained increase in the number of red fluorescent puncta (autolysosomes).
Front Pharmacol. 2022 Jan 11;12:826628
Mouse primary hepatocytes (MPHs)
12.5, 25, 50 μM
24 h
AO significantly alleviated OAPA-induced lipid accumulation in hepatocytes, dose-dependently reduced the number and size of lipid droplets, and decreased intracellular TG content.
Front Pharmacol. 2022 Jan 11;12:826628
RAW264.7 cells
6.25-50 μM
24 h
To investigate the anti-inflammatory effects of Aurantio-obtusin on LPS-induced RAW264.7 cells. Results showed that Aurantio-obtusin significantly inhibited the production of NO, PGE2, and reduced the protein expression of COX-2, TNF-α, and IL-6.
Molecules. 2018 Nov 27;23(12):3093
Human renal glomerular endothelial cells (HRGECs)
50, 100, 200 µM
48 h
To assess the cytotoxic effects of AO on HRGECs. AO caused decreased cell viability, increased LDH leakage, increased secretion of inflammatory cytokines (IL-6, TNF-α, TGF-β1, MCP-1), and decreased ZO-1 expression.
Nutrients. 2022 Nov 2;14(21):4615
Aurantio-obtusin/橙黄决明素 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6J mice
HFHS diet-induced obese mouse model
Oral gavage
10 mg/kg
Once daily for 4 weeks
AO significantly increased the weight of BAT and accelerated energy expenditure to protect the weight increase in the obese mice.
Acta Pharmacol Sin. 2023 Sep;44(9):1826-1840
C57BL/6J mice
High-fat diet and glucose-fructose water (HFSW)-induced NAFLD mouse model
Oral gavage
5, 10, 15 mg/kg
Once daily for 4 weeks
AO significantly alleviated HFSW-induced hepatic steatosis, reduced serum ALT and AST levels, decreased hepatic TG and TC content, and improved liver histology.
Front Pharmacol. 2022 Jan 11;12:826628
Sprague-Dawley (SD) rats
Hepatotoxicity model
Oral
4, 40, and 200 mg/kg
Once daily for 28 days
To study the hepatotoxic effects of Aurantio-obtusin in rats and identify potential biomarkers in urine. Results showed that 23 metabolites were identified as potential biomarkers, with 10 up-regulated and 13 down-regulated.
Front Pharmacol. 2020 Aug 12;11:1237
C57BL/6J mice
HFHS diet-induced obesity model
Oral
10 mg/kg
Once daily for 4 weeks
To investigate the effect of AO on BAT function and hepatic inflammation in obese mice, AO improved mitochondrial function in BAT and reduced hepatic inflammation.
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