Anwuligan, a natural product isolated and purified from the fruits of Schisandra chinensis, possesses therapeutic potentials against neurodegenerative diseases with oxidative stress and neuroinflammation.
Macelignan/安五脂素 细胞实验
Cell Line
Concentration
Treated Time
Description
References
3×Transgene-AD mouse primary neuron cells
20 µM
24 hours
To investigate the effect of Macelignan on Tau phosphorylation, results showed that Macelignan significantly reduced the phosphorylation levels of Tau at Ser 404 site
Front Nutr. 2022 May 18;9:892558
N2a/SweAPP cells
0, 5, 10, 15, 20 µM
24 hours
To investigate the effect of Macelignan on Aβ aggregation, results showed that Macelignan significantly reduced the expression levels of APP and Aβ
Front Nutr. 2022 May 18;9:892558
HEK293/Tau cells
0, 5, 10, 15, 20 µM
24 hours
To investigate the effect of Macelignan on Tau phosphorylation, results showed that Macelignan significantly reduced the phosphorylation levels of Tau at Ser 396, Ser 404, and Thr 231 sites
Front Nutr. 2022 May 18;9:892558
SH-SY5Y/Tau cells
0, 5, 10, 15, 20 µM
24 hours
To investigate the effect of Macelignan on Tau phosphorylation, results showed that Macelignan significantly reduced the phosphorylation levels of Tau at Ser 396, Ser 404, and Thr 231 sites
Front Nutr. 2022 May 18;9:892558
MCF-7 cells
100 µM
144 hours
To evaluate the effect of Macelignan on the proliferation of MCF-7 cells, results showed that 100 µM Macelignan increased cell proliferation to 201.07 ± 3.68%
Pharmaceutics. 2021 Jul 15;13(7):1082
H9c2 cardiomyoblast cells
50 μM
22 hours
To evaluate the protective effect of Macelignan against H2O2-induced oxidative stress, results showed that Macelignan significantly improved cell viability and reduced apoptosis
Sci Rep. 2021 Jan 12;11(1):664
HepG2 cells
0–3 mM
48h
Assess the cytotoxicity of Macelignan on HepG2 cells, showing low cytotoxicity.
Braz J Med Biol Res. 2016 Jul 4;49(7):e5313
Ascosphaera apis
1.56 mg/mL (24h), 3.125 mg/mL (48h)
24h, 48h
Evaluate the antifungal activity of Macelignan against Ascosphaera apis, showing significant growth inhibition.
Braz J Med Biol Res. 2016 Jul 4;49(7):e5313
Streptococcus sanguinis
200 µg/ml
24 hours
To evaluate the growth inhibitory effect of Macelignan on Streptococcus sanguinis, showing an inhibition zone diameter of 12.2±1.13 mm.
Oral Health Prev Dent. 2020 Jul 4;18(3):537-542
Streptococcus mutans
200 µg/ml
24 hours
To evaluate the growth inhibitory effect of Macelignan on Streptococcus mutans, showing an inhibition zone diameter of 11.8±0.67 mm.
Oral Health Prev Dent. 2020 Jul 4;18(3):537-542
Macelignan/安五脂素 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6 mice
Chronic unpredictable mild stress (CUMS) model
Intragastric administration
5 mg/kg
Daily administration for 30 days
Macelignan significantly improved CUMS-induced depression-like behaviors, including reduced immobility time in forced swimming and tail suspension tests. Additionally, Macelignan restored oxidative balance, suppressed neuroinflammation, and enhanced synaptic plasticity.
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