Didymin, a natural product isolated and purified from the herbs of Clinopodium chinense with antioxidant property, may be a potential therapeutic molecule for the treatment of neurodegenerative disorders associated with oxidative stress.
Didymin/香蜂草苷 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Human umbilical vein endothelial cells (HUVECs)
20 μM
24 h
Pre-incubation with didymin prevented the release of various inflammatory cytokines and chemokines in HG-treated HUVECs.
Biochem Pharmacol. 2018 Jun;152:1-10
Human umbilical vein endothelial cells (HUVECs)
20 μM
6 h
Pre-incubation with didymin prevented HG-induced monocyte adhesion to endothelial cells and inhibited expressions of ICAM-1 and VCAM-1 and activation of NF-κB.
Biochem Pharmacol. 2018 Jun;152:1-10
Human umbilical vein endothelial cells (HUVECs)
20 μM
3 h
Pre-incubation with didymin prevented HG-induced generation of reactive oxygen species (ROS) and malondialdehyde (MDA).
Biochem Pharmacol. 2018 Jun;152:1-10
Human umbilical vein endothelial cells (HUVECs)
10 μM and 20 μM
24 h
Pre-incubation with didymin prevented high glucose (HG)-induced loss of cell viability.
Biochem Pharmacol. 2018 Jun;152:1-10
H9c2 cardiomyocytes
5, 10, 20 μg/mL
4 h
Did pretreatment significantly maintained cell viability and reduced LDH release, alleviating DOX-induced cell injury
Chin Herb Med. 2021 Jul 15;14(1):70-78
INS-1 cells
50 µM
24 h
To evaluate the effect of Didymin on insulin secretion function in INS-1 cells. Results showed that Didymin improved insulin secretion under high glucose stimulation, reduced excessive insulin secretion under low glucose stimulation, and increased intracellular insulin storage.
Diabetol Metab Syndr. 2024 Jan 3;16(1):7
AML12 cells
50 μM
24 h
To evaluate the effect of Didymin on PA-induced lipid deposition in AML12 cells. Results showed that Didymin significantly reduced PA-induced lipid accumulation.
J Transl Med. 2023 Dec 19;21(1):921
MCF-7/ADR cells
10μM-200μM
48 h
Evaluate the chemosensitization effect of Didymin and its inclusion complexes on drug-resistant tumor cells. Results showed that Didymin significantly increased intracellular accumulation of DOX in resistant cells and enhanced the cytotoxicity of DOX.
Drug Deliv. 2020 Dec;27(1):54-65
PC12 cells
10μM-200μM
48 h
Evaluate the toxicity of Didymin and its inclusion complexes on healthy cell lines. Results showed that cell viability remained above 90% at all tested concentrations, indicating low toxicity of Didymin and its inclusion complexes to healthy cells.
Drug Deliv. 2020 Dec;27(1):54-65
H9C2 cells
10μM-200μM
48 h
Evaluate the toxicity of Didymin and its inclusion complexes on healthy cell lines. Results showed that cell viability remained above 90% at all tested concentrations, indicating low toxicity of Didymin and its inclusion complexes to healthy cells.
Drug Deliv. 2020 Dec;27(1):54-65
HUVEC cells
10μM-200μM
48 h
Evaluate the toxicity of Didymin and its inclusion complexes on healthy cell lines. Results showed that cell viability remained above 90% at all tested concentrations, indicating low toxicity of Didymin and its inclusion complexes to healthy cells.
Drug Deliv. 2020 Dec;27(1):54-65
Didymin/香蜂草苷 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Male BALB/c mice
DOX-induced cardiotoxicity model
Intraperitoneal injection
2, 4, 8 mg/kg
Once daily for 7 days
Did pretreatment significantly restored body weight, inhibited DOX-induced increase of LDH, CK, AST, and alleviated myocardial tissue injury and oxidative stress
Chin Herb Med. 2021 Jul 15;14(1):70-78
C57BL/6J mice
CCl4-induced liver fibrosis model
Intragastric administration
2, 1, or 0.5 mg/kg
6 weeks
Didymin significantly attenuated CCl4-induced hepatic injury and fibrogenesis, as evidenced by the ameliorative pathological tissue, low transaminase activity, and decreased collagen accumulation.
Drug Des Devel Ther. 2022 Jun 7;16:1713-1729
C57BL/6J mice
High-fat diet-induced impaired glucose tolerance (IGT) mouse model
Intraperitoneal injection
0.8 mg/kg
Once daily for three weeks
To evaluate the effect of Didymin on pancreatic beta cell function in high-fat diet-induced IGT mice. Results showed that Didymin reduced postprandial glycemia and enhanced 30-minute postprandial insulin levels, improving pancreatic beta cell insulin secretion function.
Diabetol Metab Syndr. 2024 Jan 3;16(1):7
C57BL/6J mice
High-fat diet-induced MAFLD mouse model
Intraperitoneal injection
0.8 mg/kg
Once daily for three weeks
To evaluate the therapeutic effect of Didymin on high-fat diet-induced MAFLD mice. Results showed that Didymin significantly reduced hepatic lipid deposition and serum lipid levels.
J Transl Med. 2023 Dec 19;21(1):921
SD rats
Not used
Oral
25 mg/kg
Single dose
Evaluate the pharmacokinetic properties of Didymin and its inclusion complexes. Results showed that the bioavailability of Didymin/HP-β-CD inclusion complex was significantly higher than that of free Didymin and Didymin/β-CD inclusion complex.
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