Otilonium bromide (OB) is a smooth muscle relaxant used in the treatment of irritable bowel syndrome. Otilonium bromide inhibited the generation of ACh-induced calcium signals in a dose dependent manner (IC50=880 nM)[3]. In experiments with recordings in the same cells of whole-cell IDMPP, Na+ currents (INa) and Ca2+ currents (ICa), 1 mM otilonium blocked 87% IDMPP, 7% INa and 13% ICa. Otilonium inhibited the K+-evoked catecholamine secretory response of superfused bovine chromaffin cells with an IC50 of 10 mM and inhibited the secretory responses induced by 10 s pulses of 50 mM DMPP with an IC50 of 7.4 nM[4].
Otilonium bromide/奥替溴铵 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Candida albicans
1 μg/mL
4 hours
To observe the distribution of ergosterol on the plasma membrane using filipin staining. Results showed that after OTB treatment, the fluorescence of ergosterol on the plasma membrane weakened, while intracellular fluorescence increased, indicating a decrease in ergosterol distribution on the plasma membrane.
Adv Sci (Weinh). 2024 Sep;11(35):e2406473
Candida albicans
1 μg/mL
4 hours
To investigate the inhibitory effect of OTB on C. albicans and its impact on the expression of ergosterol biosynthesis genes. Results showed that OTB significantly upregulated the expression of ERG1, ERG2, ERG4, ERG5, ERG6, ERG7, ERG9, ERG10, ERG11, ERG12, ERG13, ERG24, ERG25, ERG26, and ERG27 genes.
Adv Sci (Weinh). 2024 Sep;11(35):e2406473
Otilonium bromide/奥替溴铵 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Gastrointestinal C. albicans infection model
Intragastric administration
50 mg/kg
Administered daily until the end of the observation period
To evaluate the therapeutic effect of OTB on invasive candidiasis. Results showed that OTB significantly extended the median survival time of mice (from 5 days to 30 days) and improved the survival rate from 0% to 62.5%.
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