There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Jatrorrhizine is an alkaloid isolated from Coptis chinensis with neuroprotective. In vitro uptake tests showed that jatrorrhizine strongly inhibited PMAT-mediated MPP+ uptake with an IC50 value of 1.05 μM and reduced 5-HT and NE uptake mediated by hOCT2, hOCT3 and hPMAT with IC50 values of 0.1-1 μM (for OCT2 and OCT3) and 1-10 μM (for PMAT)[3]. Moreover, jatrorrhizine distinctly inhibited the proliferation of MDA-MB-231, MCF-7 and 4T1 cells with IC50 values of 11.08 ± 1.19 μM, 17.11 ± 4.54 μM and 22.14 ± 2.87 μM, induced mitochondrial dysfunction and early apoptosis involving mitochondrial apoptotic pathway[4]. JAT (Jatrorrhizine) inhibited the proliferation of HCT-116 and HT-29 cells with IC50 values of 6.75±0.29 μM and 5.29±0.13 μM, respectively, for 72 hrs. In addition, JAT inhibited Wnt signaling pathway by reducing β-catenin and increasing GSK-3β expressions. In HCT-116 nude mice xenograft model, JAT inhibited tumor growth and metastasis, and induced apoptosis of tumor cells[5]. Jatrorrhizine dose-dependently (0.1, 0.3 and 1 mg/kg) offset delayed gastric emptying and intestinal transit (geometric centre and the migration of Evans blue) in postoperative ileus[6]. JAT administration could alleviate the learning and memory deficits in AD. Furthermore, JAT treatment reduced the levels of Aβ plaques in the cortex and hippocampus of APP/PS1 double-transgenic mice[7].
Jatrorrhizine/药根碱 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Caco-2 cells
10 μM
120 minutes
To evaluate the permeability and efflux capacity of Jatrorrhizine in Caco-2 cell monolayers. Results showed a net efflux value of 1.73 ± 0.42 × 10−6 cm/s for Jatrorrhizine.
Acta Pharmacol Sin. 2019 Jan;40(1):133-142
HepG2 cells
20 μM
24 hours
To examine the effect of Jatrorrhizine on AMPK activity, results showed that Jatrorrhizine had no significant effect on AMPK activity.
J Transl Med. 2011 May 15;9:62
HepG2 cells
40 μM
8 hours
To examine the effect of Jatrorrhizine on LDLR mRNA expression, results showed that Jatrorrhizine had no significant effect on LDLR mRNA expression.
J Transl Med. 2011 May 15;9:62
HepG2 cells
20 μM
8 hours
To examine the effect of Jatrorrhizine on InsR mRNA expression, results showed that Jatrorrhizine increased InsR mRNA level by around 1.5 folds.
J Transl Med. 2011 May 15;9:62
MNV-infected RAW264.7 macrophages
10, 20, 40 μM
48 hours
To evaluate the effect of Jatrorrhizine on cytotoxicity caused by MNV infection, results showed that Jatrorrhizine significantly inhibited the reduction in cell viability caused by MNV infection
Vaccines (Basel). 2023 Jan 12;11(1):164
RAW264.7 macrophages
5, 10, 20, 40, 80, 160 μM
48 hours
To evaluate the effect of Jatrorrhizine on the viability of RAW264.7 macrophages, results showed that Jatrorrhizine had no significant effect on cell viability at concentrations of 5-160 μM
Vaccines (Basel). 2023 Jan 12;11(1):164
human umbilical cord vein endothelial cells (HUVECs)
1 μM
48 hours
To evaluate the protective effect of Jatrorrhizine on HUVECs under high glucose conditions, results showed that JAT reversed high glucose-induced downregulation of Akt/eNOS phosphorylation, alleviated ER stress and oxidative stress, and increased NO release.
To investigate the effect of Jatrorrhizine on LPS-induced inflammatory response in HUVECs, results showed that Jatrorrhizine inhibited the HMGB1/NF-κB/NLRP3 signaling pathway.
Phytomedicine. 2022 Jun;100:154083
Jatrorrhizine/药根碱 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6J mice
High-fat diet-induced obese and diabetic mouse model
Oral
50 mg/kg/day
Once daily for 5 weeks
To evaluate the protective effect of Jatrorrhizine on vascular function in diabetic and obese mice, results showed that JAT improved endothelium-dependent relaxations, reduced blood pressure, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, and improved plasma lipid profile.
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