L-755507 is characterized as a potent and selective β3 adrenergic receptor partial agonist with EC50 of 0.43 nM. It is also recently identified to enhance CRISPR-mediated homology-directed repair (HDR) efficiency in human induced pluripotent stem cells (iPSCs) and other cell types.
L755507 细胞实验
Cell Line
Concentration
Treated Time
Description
References
D341 cells
4.64 ± 0.13 μM (IC50)
48 hours
To evaluate the cytotoxic effect of L755507 on D341 cells, results showed that L755507 effectively inhibited cell growth with an IC50 value of 4.64 ± 0.13 μM
J Biol Chem. 2021 Jul;297(1):100903.
HL-60 cells
2.87 ± 0.13 μM (IC50)
48 hours
To evaluate the cytotoxic effect of L755507 on HL-60 cells, results showed that L755507 effectively inhibited cell growth with an IC50 value of 2.87 ± 0.13 μM
J Biol Chem. 2021 Jul;297(1):100903.
HT-29 cells
1.79 ± 0.13 μM (IC50)
48 hours
To evaluate the cytotoxic effect of L755507 on HT-29 cells, results showed that L755507 effectively inhibited cell growth with an IC50 value of 1.79 ± 0.13 μM
J Biol Chem. 2021 Jul;297(1):100903.
Porcine fetal fibroblasts
40 μM
48 hours
To evaluate the effect of L755507 on HDR efficiency. Results showed that 40 μM L755507 improved HDR efficiency by approximately 2-fold.
Sci Rep. 2017 Aug 21;7(1):8943.
Porcine fetal fibroblasts
5 μM, 10 μM, 20 μM, 40 μM
48 hours
To evaluate the effect of L755507 on cell viability and cell cycle. Results showed that L755507 significantly decreased the proportion of cells in the G2/M phase at 10 μM or 40 μM and increased the S-phase cells at 10 μM compared with the DMSO-treated cells.
Sci Rep. 2017 Aug 21;7(1):8943.
Porcine fetal fibroblasts (PFFs)
5 µM
3 days
To improve the efficiency of CRISPR RNP-mediated precise gene editing, results showed that L755507 increased the precise gene editing efficiency by 1.91-fold.
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