There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Guanabenz Acetate is an alpha2 adrenergic receptor agonist shown to interact with a regulatory subunit of the protein phosphatase, Pp1/Gadd34, and selectively disrupt the dephosphorylation of the alpha subunit of eukaryotic initiation factor 2.Guanabenz treatment accelerated ALS-like disease progression in a strain of mutant SOD1 transgenic ALS(Amyotrophic Lateral Sclerosis) mice[3]. Guanabenz Acetate is capable of markedly increasing the cellular internalization and target mRNA silencing of hydrophobically modified siRNAs (hsiRNAs), yielding a ∼100-fold decrease in hsiRNA IC50 (from 132 nM to 2.4 nM)[4].
Guanabenz Acetate/氯苄氨胍醋酸盐 细胞实验
Cell Line
Concentration
Treated Time
Description
References
DLD1 cells
50 µM
48 hours
GBZ treatment reduced the expression of β-catenin target genes
Nat Commun. 2022 Feb 3;13(1):674.
HeLa cells
0-200 µM
24 hours
To assess the impact of Guanabenz on HeLa cell viability, showing toxicity at concentrations of 125 µM and above.
Nucleic Acids Res. 2015 Oct 15;43(18):8664-72.
HCM-BROD-0106-C71 cells
50 µM
24 hours
To evaluate the cytotoxicity of Guanabenz on patient-derived glioblastoma cells and its combined effect with Sunitinib. Results showed that Guanabenz enhanced the cytotoxicity of Sunitinib on patient-derived glioblastoma cells.
Neurotherapeutics. 2021 Apr;18(2):1371-1392.
T98G-R cells
50 µM
24 hours
To evaluate the cytotoxicity of Guanabenz on TMZ-resistant glioblastoma cells and its combined effect with Sunitinib. Results showed that Guanabenz enhanced the cytotoxicity of Sunitinib on TMZ-resistant glioblastoma cells.
Neurotherapeutics. 2021 Apr;18(2):1371-1392.
A172 cells
50 µM
24 hours
To evaluate the cytotoxicity of Guanabenz on glioblastoma cells and its combined effect with Sunitinib. Results showed that Guanabenz enhanced the cytotoxicity of Sunitinib on glioblastoma cells and reduced Sunitinib-induced autophagy.
Neurotherapeutics. 2021 Apr;18(2):1371-1392.
U-87MG cells
50 µM
24 hours
To evaluate the cytotoxicity of Guanabenz on glioblastoma cells and its combined effect with Sunitinib. Results showed that Guanabenz enhanced the cytotoxicity of Sunitinib on glioblastoma cells and reduced Sunitinib-induced autophagy.
Neurotherapeutics. 2021 Apr;18(2):1371-1392.
Human placental BeWo cell line
5 µM
24 hours
To assess the effect of GADD34 inhibition on ER stress-induced reduction in mTORC1 activity, results showed that guanabenz attenuated the tunicamycin-mediated increase in CHOP protein levels and reduction in mTORC1 activity
Cell Commun Signal. 2023 Nov 13;21(1):326.
SW480 cells
50 µM
48 hours
GBZ treatment decreased β-catenin levels, which was markedly attenuated by conductin knockout
Guanabenz protected dOPCs from IFN-γ-mediated apoptotic death, restoring cell survival to control levels.
Nat Commun. 2015 Mar 13;6:6532.
J774.1 cells
50 µM
6 hours
Measure mRNA expression levels of inflammatory cytokines, results showed that Guanabenz blocked LPS-induced increases in IFN-γ, TNF-α, IL-6, IL-1β, and COX-2 mRNA expression
mBio. 2019 Apr 30;10(2):e00381-19.
Primary macrophages
10 µM
6 hours
Suppressed LPS-induced upregulation of IL1β, IL6, TNFα, and Cox2 mRNA expression
Int J Mol Sci. 2016 May 5;17(5):674.
RAW264.7 macrophages
10 µM
6 hours
Suppressed LPS-induced upregulation of IL1β, IL6, TNFα, and Cox2 mRNA expression
Int J Mol Sci. 2016 May 5;17(5):674.
Bone marrow-derived dendritic cells
50 µM
8 hours
To evaluate the effect of GBZ on IL-10 production in LPS-stimulated dendritic cells. Results showed that GBZ increased IL-10 production.
Front Immunol. 2017 Jun 13;8:679.
U2OS cells
50 µM
Overnight
GBZ treatment induced condensation of transiently expressed conductin
Nat Commun. 2022 Feb 3;13(1):674.
HMC-1.1 mast cells
10 µM
Suppressed PMA-induced upregulation of TNFα and IL13 mRNA expression
Int J Mol Sci. 2016 May 5;17(5):674.
Jurkat T lymphocytes
5 or 10 µM
Suppressed PMA-induced upregulation of IL2 and IFNγ mRNA expression
Int J Mol Sci. 2016 May 5;17(5):674.
Guanabenz Acetate/氯苄氨胍醋酸盐 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
MPZmutant mice and SOD1mutant mice
Oral
1 mg/kg or 5 mg/kg
Twice a day or once a day for several weeks to months
Sephin1 prevented the motor, morphological, and molecular defects of MPZmutant and SOD1mutant mice without adverse effects.
Science. 2015 Apr 10;348(6231):239-42
NOD-SCID mice
U-87MG glioblastoma xenograft model
Peritumoral injection
10 mg/kg
3 injections on days 14, 21, and 28
To evaluate the therapeutic effect of combined treatment with Guanabenz and Sunitinib on glioblastoma xenograft model. Results showed that combined treatment significantly inhibited tumor growth, reduced Ki67 expression, and decreased autophagy-related markers.
Neurotherapeutics. 2021 Apr;18(2):1371-1392.
BALB/cJ and C57BL/6J mice
Chronic Toxoplasma infection model
Oral gavage
10 mg/kg
Once daily for 3 to 6 weeks
Evaluate the effects of Guanabenz on brain cyst burden and neuroinflammation in chronically Toxoplasma-infected mice. Results showed that Guanabenz significantly reduced brain cyst burden (~75%) in BALB/cJ mice via i.p. injection and reversed Toxoplasma-induced hyperactivity, which correlated with reduced neuroinflammation. However, in C57BL/6J mice, Guanabenz increased cyst burden but still reversed hyperactivity.
mBio. 2019 Apr 30;10(2):e00381-19.
C57BL/6 mice
D-galactosamine/LPS-induced liver damage model
Intraperitoneal injection
2 mg/kg
Single injection, observed for 48 hours
To evaluate the effect of GBZ on d-galN/LPS-induced liver damage and mortality. Results showed that GBZ significantly improved Mice survival, reduced liver damage, increased IL-10 levels, and inhibited TNF-α production.
Front Immunol. 2017 Jun 13;8:679.
Drosophila
OPMD model
Oral
3 mM
Daily administration until adulthood
Alleviate OPMD phenotypes, including muscle degeneration and nuclear inclusion formation
EMBO Mol Med. 2011 Jan;3(1):35-49
Mice
GFAP/tTA; TRE/IFN-γ transgenic mice
Intraperitoneal injection
4 mg/kg
Daily from P7 to P18
Guanabenz protected oligodendrocytes and myelin from the detrimental effects of IFN-γ, restoring myelination to levels observed in wild type littermates.
Nat Commun. 2015 Mar 13;6:6532.
C57BL/6 mice
Tunicamycin-induced ER stress model
Intraperitoneal injection
4 mg/kg
For 3 consecutive days
Sephin1 significantly suppressed tunicamycin-induced renal tubular cell death, improved survival, and markedly suppressed nuclear translocation of CHOP.
Cell Death Dis. 2025 Feb 19;16(1):117.
C57BL/6 mice
OVX-induced osteoporosis model
Intraperitoneal injection
4, 8 mg/kg
Every two days for 6 weeks
Inhibition of OVX-induced osteoporosis
Acta Pharmacol Sin. 2024 Apr;45(4):790-802.
BALB/cJ and C57BL/6J mice
Chronic Toxoplasma infection model
Intraperitoneal injection
5 mg/kg
Once daily for 3 to 6 weeks
Evaluate the effects of Guanabenz on brain cyst burden and neuroinflammation in chronically Toxoplasma-infected mice. Results showed that Guanabenz significantly reduced brain cyst burden (~75%) in BALB/cJ mice via i.p. injection and reversed Toxoplasma-induced hyperactivity, which correlated with reduced neuroinflammation. However, in C57BL/6J mice, Guanabenz increased cyst burden but still reversed hyperactivity.
mBio. 2019 Apr 30;10(2):e00381-19.
BALB/c nude mice
SW480 xenograft model
Oral
50 µM
Daily for 20 days
GBZ treatment reduced tumor growth by about 60%
Nat Commun. 2022 Feb 3;13(1):674.
Mice
Tsc1 conditional knockout mice
Intraperitoneal injection
8 mg/kg
Once daily from P7 to P14
To evaluate the effect of Guanabenz on oligodendrocyte survival and myelination in Tsc1 mutant mice, results showed that Guanabenz treatment partially rescued myelination defects
Nat Commun. 2016 Jul 15;7:12185
Mice (C57BL/6J)
Experimental autoimmune encephalomyelitis (EAE) model
Intraperitoneal injection
8 mg/kg
Once daily, starting from PID 7 until the end of the study
To evaluate the effect of Sephin1 on the disease progression in EAE model mice. Results showed that Sephin1 significantly delayed disease onset, reduced oligodendrocyte and axon loss, and decreased T cell presence in the CNS.
Brain. 2019 Feb 1;142(2):344-361
C57BL/6 mice
G93A mtSOD1 transgenic mice
Intraperitoneal injection
8 mg/kg
Three times a week starting at 60 days of age
Guanabenz significantly delayed disease onset, prolonged the duration of the early phase of disease, and extended survival of G93A mice.
United States, Indiana
... 展开 >>
Indiana University Health Hospital
Indianapolis, Indiana, United States, 46202
Indiana University Melvin and Bren Simon Cancer Center
Indianapolis, Indiana, United States, 46202
Sidney & Lois Eskenazi Hospital
Indianapolis, Indiana, United States, 46202
Spring Mill Medical Center
Indianapolis, Indiana, United States, 46290 收起 <<
United States, Indiana
... 展开 >>
Indiana University Health Hospital
Indianapolis, Indiana, United States, 46202
Indiana University Melvin and Bren Simon Cancer Center
Indianapolis, Indiana, United States, 46202
Sidney & Lois Eskenazi Hospital
Indianapolis, Indiana, United States, 46202
Spring Mill Medical Center
Indianapolis, Indiana, United States, 46290 收起 <<
搜索
HazMat Fee +
