Dehydrocorydaline, a natural product isolated and purified from the tubers of Corydalis ambigua, inhibits MCF-7 cell proliferation by inducing apoptosis mediated by regulating Bax/Bcl-2, activating caspases as well as cleaving PARP.
Dehydrocorydaline/脱氢紫堇碱 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Bone marrow-derived macrophages
50 µM, 100 µM
12 hours
Dehydrocorydaline significantly downregulated the mRNA levels of IL-1β and IL-18 in a time- and concentration-dependent manner.
Acta Pharmacol Sin. 2022 Jun;43(6):1408-1418.
Bone marrow-derived macrophages
100 µM
24 hours
Through RNA-seq analysis, Dehydrocorydaline significantly downregulated 825 genes, including 260 upregulated genes and 565 downregulated genes, indicating its inhibition of macrophage inflammatory responses.
Acta Pharmacol Sin. 2022 Jun;43(6):1408-1418.
Human chondrocytes
10 µM
48 hours
Dehydrocorydaline significantly promoted the cell activity of normal human chondrocytes without showing cytotoxicity. Moreover, 10 and 20 μM DHC clearly restored cell proliferation, inhibited mRNA and protein expression levels of type I collagen, MMP 1/13, and Cox2, and further increased those of aggrecan and type II collagen in the TNF-α-treated human chondrocytes.
Int J Mol Sci. 2022 Jun 30;23(13):7268.
Human chondrocytes
20 µM
48 hours
Dehydrocorydaline significantly promoted the cell activity of normal human chondrocytes without showing cytotoxicity. Moreover, 10 and 20 μM DHC clearly restored cell proliferation, inhibited mRNA and protein expression levels of type I collagen, MMP 1/13, and Cox2, and further increased those of aggrecan and type II collagen in the TNF-α-treated human chondrocytes.
Int J Mol Sci. 2022 Jun 30;23(13):7268.
Dehydrocorydaline/脱氢紫堇碱 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Sprague-Dawley rats
Anterior cruciate ligament transection (ACLT)-induced osteoarthritis model
Intra-articular injection
1 mM
Daily for the third week, then once every 3 days for the next 3 weeks
Dehydrocorydaline significantly attenuated OA development, inhibited the enzymatic hydrolysis of ECM, and reduced phosphorylated JAK1 and STAT3 protein expression in vivo after ACLT for 6 weeks.
Int J Mol Sci. 2022 Jun 30;23(13):7268.
ApoE−/− mice
Atherosclerosis model
Intraperitoneal injection
5 mg/kg
Daily for 12 weeks
Dehydrocorydaline significantly inhibited the development of atherosclerosis, improved aortic compliance, and increased plaque stability. Additionally, Dehydrocorydaline attenuated systemic and vascular inflammation in ApoE?/? mice.
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