Ginsenoside Ro, a natural product isolated and purified from the root of Panax ginseng C. A. Mey., is reported to have anti-infllammtory, anti-hepatitic activities, and showed inhibitory activity against 5αR with IC(50) value of 259.4 μm
Ginsenoside Ro/人参皂苷Ro 细胞实验
Cell Line
Concentration
Treated Time
Description
References
EC-9706 cells
50 μM
12 hours
Ro treatment resulted in a dramatic accumulation of LC3B-II, indicating inhibition of autophagic flux.
Autophagy. 2016 Sep;12(9):1593-613
TE-1 cells
50 μM
12 hours
Ro treatment resulted in a dramatic accumulation of LC3B-II, indicating inhibition of autophagic flux.
Autophagy. 2016 Sep;12(9):1593-613
ECA-109 cells
50 μM
12 hours
Ro blocked the autophagosome-lysosome fusion process by raising lysosomal pH and attenuating lysosomal cathepsin activity, resulting in the accumulation of the autophagosome marker MAP1LC3B/LC3B and SQSTM1/p62.
Autophagy. 2016 Sep;12(9):1593-613
RAW264.7 macrophages
10-400 μM
24 hours
To assess the effect of Ginsenoside Ro on cell viability. Results showed that Ginsenoside Ro was non-toxic to RAW264.7 cells at concentrations ranging from 10-400 μM.
J Ginseng Res. 2022 Jan;46(1):156-166
human platelets
100-300 μM
5 min
Inhibited fibrinogen binding to αIIb/β3 via cAMP-dependent phosphorylation of VASP (Ser157)
J Ginseng Res. 2016 Oct;40(4):359-365
human platelets
50-300 μM
3 min
Inhibited thrombin-induced platelet aggregation in a dose-dependent manner by reducing light transmission
J Ginseng Res. 2016 Oct;40(4):359-365
human platelets
50-300 μM
3 minutes
To investigate the effect of G-Ro on thrombin-induced platelet aggregation and TXA2 production. G-Ro dose-dependently inhibited platelet aggregation and TXB2 levels.
J Ginseng Res. 2019 Apr;43(2):236-241
Raw 264.7 mouse macrophage cells
50 μM, 100 μM, 200 μM
1 h pretreatment followed by 24 h LPS treatment
GRo significantly increased cell viability, reduced ROS and NO levels, decreased expression of inflammatory cytokines iNOS and COX-2, and exerted anti-inflammatory effects via upregulation of HO-1
J Ginseng Res. 2015 Oct;39(4):365-70
Ginsenoside Ro/人参皂苷Ro 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6 mice
LPS-induced acute lung injury model
Oral
20, 40, 80 mg/kg
Consecutive five days
To evaluate the protective effect of Ginsenoside Ro on LPS-induced acute lung injury. Results showed that Ginsenoside Ro significantly attenuated pathological damage in lung tissue and reduced the expression levels of TNF-α, IL-6, and IL-1β.
J Ginseng Res. 2022 Jan;46(1):156-166
BALB/c mice
Lethal HVJ infection model
Oral
1 mg/mouse
Administered 3, 2, and 1 day before infection
Ginsenoside Ro showed significant protective activity against HVJ infection with a survival rate of 42.8%.
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