(+)-Praeruptorin A is a natural product isolated and purified from the roots of Peucedanum praeruptorum Dunn., which exerted distinct relaxant effects on isolated rat aorta rings, which might be mainly attributed to nitric oxide synthesis catalyzed by endothelial nitric oxide synthase.
Praeruptorin A/白花前胡甲素A 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Rat thoracic aorta rings
0.277-204 µM
10 minutes
PA at concentrations as low as 2.77 × 10−7 M had a vasorelaxant effect in intact rat thoracic aorta rings; however, this effect was significantly reduced after removal of the vascular endothelium.
Bioengineered. 2022 Apr;13(4):10038-10046
HEK293T cells
10 µM
2 days
To evaluate the effect of Praeruptorin A on NFATc1 transcriptional activity, results showed that Praeruptorin A significantly inhibited RANKL-induced NFATc1 transcriptional activity.
PLoS One. 2014 Feb 21;9(2):e88974
HeLa cells
20 µM
2 hours pretreatment, then TPA treatment for different times
PA inhibited TPA-induced ERK1/2 activation, MMP-2 expression, migration and invasion of HeLa cells
Int J Mol Sci. 2017 Dec 21;19(1):10
HeLa cells
20 µM
24 hours
PA suppressed ERK1/2 activation and enhanced the effect of PD98059 (a specific MEK1/2 inhibitor) in downregulating MMP-2 and upregulating TIMP-2
Int J Mol Sci. 2017 Dec 21;19(1):10
HeLa cells
20 and 30 µM
24 hours
PA significantly reduced MMP-2 expression and increased TIMP-2 expression
Int J Mol Sci. 2017 Dec 21;19(1):10
HeLa cells
0 to 30 µM
24 hours
PA induced cell cycle arrest at G0/G1 phase in HeLa cells, upregulated Rb, p16, p21 and p27 proteins, and downregulated cyclin D1 and Skp2 proteins
Int J Mol Sci. 2017 Dec 21;19(1):10
SiHa cells
0 to 50 µM
24 hours
PA significantly inhibited the proliferation and colony formation of SiHa cells
Int J Mol Sci. 2017 Dec 21;19(1):10
HeLa cells
0 to 50 µM
24 hours
PA significantly inhibited the proliferation and colony formation of HeLa cells
Int J Mol Sci. 2017 Dec 21;19(1):10
H1299 cells
0, 10, 20, 30, 40, 50 µM
24 hours
Evaluate the effect of Praeruptorin A on cell viability, results showed no significant effect on H1299 cells
Molecules. 2020 Apr 1;25(7):1625
A549 cells
0, 10, 20, 30, 40, 50 µM
24 hours
Evaluate the effect of Praeruptorin A on cell viability, results showed no significant effect on A549 cells
Molecules. 2020 Apr 1;25(7):1625
SGC7901 human gastric cancer cells
10, 50, 100 µM
24 hours
Evaluate the cytotoxicity of Praeruptorin A on SGC7901 cells. Results showed that Praeruptorin A at 10, 50, and 100 μM increased LDH release, with a maximum release rate of 29.7%.
Molecules. 2010 Nov 9;15(11):8060-71
SGC7901 human gastric cancer cells
10, 50, 100 µM
24 hours
Evaluate the inhibitory effect of Praeruptorin A on SGC7901 cell proliferation. Results showed that Praeruptorin A inhibited cell growth by 19.9%, 24.1%, and 33.7% at concentrations of 10, 50, and 100 μM, respectively.
Molecules. 2010 Nov 9;15(11):8060-71
Bone marrow-derived macrophages (BMMs)
10 µM
4 days
To evaluate the effect of Praeruptorin A on RANKL-induced osteoclast differentiation, results showed that Praeruptorin A significantly inhibited the formation of TRAP-positive multinucleated cells and TRAP activity.
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