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HazMat fee for 500 gram (Estimated)
Excepted Quantity
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Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
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Accessible (Haz class 3, 4, 5 or 8), International
Chelerythrine, a natural product isolated and purified from the herbs of Chelidonium majus with potent antimanic, antiproliferatvie and antitumor effect, can inhibit telomerase activity, and is a well-known protein kinase C inhibitor.
Chelerythrine/白屈菜红碱 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Hepatic stellate cells
0-5 µM
1 hour
Enhanced leptin signaling, observed elevated tyrosine phosphorylation of JAK2
Nat Commun. 2018 Jan 18;9(1):283.
293T cells
2 µM
1 hour
Enhanced insulin signaling, observed enhanced phosphorylation of the insulin receptor β-subunit (IR-β) and activation of AKT
Nat Commun. 2018 Jan 18;9(1):283.
Aplysia sensory and motor neuron cocultures
10 µM
1 hour
Inhibition of PKM Apl III reversed 5HT-induced synaptic growth, returning varicosity numbers to pretraining levels
Elife. 2014 Nov 17;3:e03896.
Rat aortic vascular smooth muscle cells (VSMCs)
100 µM
1 hour
Chelerythrine attenuated the dexmedetomidine-induced PKC phosphorylation
Int J Mol Sci. 2016 Sep 30;17(10):1663.
Rat aortic vascular smooth muscle cells (VSMCs)
100 µM
1 hour
Chelerythrine attenuated the dexmedetomidine-induced phosphorylation of MLC20 at Ser19
Int J Mol Sci. 2016 Sep 30;17(10):1663.
Rat aortic vascular smooth muscle cells (VSMCs)
100 µM
1 hour
Chelerythrine attenuated the dexmedetomidine-induced phosphorylation of CPI-17 at Thr38
Int J Mol Sci. 2016 Sep 30;17(10):1663.
LO2 cells
10 µM
24 hours
CHE had minimal cytotoxic effects on normal LO2 cells.
J Cell Mol Med. 2020 Jan;24(1):50-60.
Jurkat WT cells
1 µg/mL
48 hours
After CHE treatment, 45% of Jurkat WT cells died.
Int J Mol Sci. 2023 Oct 20;24(20):15405.
RAW264.7 cells
0.5, 1, or 2 µM
1 hour
To evaluate the inhibitory effect of CH on LPS-induced inflammatory responses. Results showed that CH pretreatment significantly inhibited LPS-induced production of TNF-α, IL-6, and IL-1β.
Front Pharmacol. 2018 Sep 26;9:1047.
Human B-lymphocytes
10 µM
15 minutes
To study the effect of chelerythrine on ATP- and PMA-induced PLD activity, results showed chelerythrine inhibited ATP-induced PLD activity by 94.2±21.9% and PMA-induced PLD activity by 68.2±7.4%
Br J Pharmacol. 2004 Jul;142(6):1015-9.
A375 cells
1 µg/mL
2 and 3 hours
After CHE treatment, A375 cells showed an increase in cPARP (apoptosis marker), indicating apoptosis induction.
Int J Mol Sci. 2023 Oct 20;24(20):15405.
Human erythrocytes
1-10 µM
2 hours
Chelerythrine was able to partially inhibit costunolide-induced phosphatidylserine exposure and cell shrinkage
Apoptosis. 2020 Oct;25(9-10):674-685.
786-O cells
6, 9, 12 µM
20 hours
CHE induced G2/M cell cycle arrest and apoptosis in 786-O cells by increasing Cle-PARP expression and decreasing the Bcl-2/Bax ratio.
J Cell Mol Med. 2020 Jan;24(1):50-60.
Caki cells
6, 9, 12 µM
20 hours
CHE induced G2/M cell cycle arrest and apoptosis in Caki cells by increasing Cle-PARP expression and decreasing the Bcl-2/Bax ratio.
J Cell Mol Med. 2020 Jan;24(1):50-60.
HepG2 cells
1.25-10 µM
24 hours
CHE induces apoptotic cell death in HepG2 cells involving the inhibition of Akt pathway and the activation of oxidative stress and mitochondrial apoptotic pathway
Antioxidants (Basel). 2022 Sep 18;11(9):1837.
Non-small cell lung cancer NCI-H1299 cells
10, 15, 20 µM
24 hours
To study the effect of CHE on cell viability, results showed CHE significantly reduced the viability of NCI-H1299 cells in a concentration-dependent manner
Redox Biol. 2017 Aug;12:367-376.
Non-small cell lung cancer A549 cells
10, 15, 20 µM
24 hours
To study the effect of CHE on cell viability, results showed CHE significantly reduced the viability of A549 cells in a concentration-dependent manner
Redox Biol. 2017 Aug;12:367-376.
NCI-N87 cells
3.81 µM (IC50)
24 hours
To evaluate the cytotoxicity of Chelerythrine on NCI-N87 cells, results showed Chelerythrine significantly reduced cell viability.
Molecules. 2023 Sep 28;28(19):6842.
Mechanically skinned skeletal muscle fibres of the rat
12 µM
30 s
Chelerythrine (12 μM) was shown to restore ECC in these fibres. Restored force responses were similar in peak but significantly broadened compared to initial control responses. Early exposure to chelerythrine prevented run-down of DIFRs. Chelerythrine also induced spontaneous force responses in some fibres.
Br J Pharmacol. 2003 Feb;138(3):417-26.
MKN45 cells
5 µM
4 hours
Chelerythrine significantly inhibited TXNRD1 activity in MKN45 cells.
Molecules. 2023 Sep 28;28(19):6842.
Jurkat CASP3/7/6-/- cells
1 µg/mL
48 hours
After CHE treatment, 13% of Jurkat CASP3/7/6-/- cells died.
Int J Mol Sci. 2023 Oct 20;24(20):15405.
Jurkat FADD-/- cells
1 µg/mL
48 hours
After CHE treatment, 81% of Jurkat FADD-/- cells died.
Int J Mol Sci. 2023 Oct 20;24(20):15405.
Human B-lymphocytes
5 µM and 10 µM
5 minutes
To study the effect of chelerythrine on ATP concentration-effect curves, results showed chelerythrine inhibited P2X7 receptor in a noncompetitive manner
Br J Pharmacol. 2004 Jul;142(6):1015-9.
Human B-lymphocytes
10 µM
5 minutes
To examine the effect of chelerythrine on P2X7 receptor function, results showed chelerythrine inhibited ATP-induced 86Rb+ efflux by 73.4±3.5%
Br J Pharmacol. 2004 Jul;142(6):1015-9.
Chelerythrine/白屈菜红碱 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Rat
Isolated perfused heart model
Perfusion
10 mM
Started 5 min before ischemia, infused for 20 min together with 5–10-sP
Chelerythrine abrogated the improvement in mitochondrial function induced by 5–10-sP, including ADP-stimulated respiration, ATP production, and prevention of ROS formation.
Front Pharmacol. 2020 May 5;11:545
Aplysia californica
Long-term sensitization model of siphon-withdrawal reflex
Intrahemocoel injection
200 µl/100 g
Single injection
PKM Apl III inhibition erased behavioral expression of long-term sensitization memory, but memory could be reinstated with additional training
Elife. 2014 Nov 17;3:e03896.
C57Bl6/J mice
High fat diet-induced obesity model
Intraperitoneal injection
3 mg/kg
Once daily for 30 days
Improved metabolism, promoted insulin and leptin signaling, and reduced body weight
Nat Commun. 2018 Jan 18;9(1):283.
Sprague-Dawley rats
Subarachnoid hemorrhage (SAH) model
Cisterna magna injection
5 µM
Injected once 2 days before SAH induction and on day 3 and day 5 after SAH induction
To investigate the effect of PKC inhibitors on SAH-induced increase in Cx43 expression. Results showed that Cx43 expression in basilar arteries significantly increased at day 7 post-SAH, and pretreatment with PKC inhibitors (Chelerythrine or GF 109203X) reversed this increase.
J Transl Med. 2019 Dec 30;17(1):433
BALB/c mice
LPS-induced acute lung injury model
Gavage
5 or 10 mg/kg
7 consecutive days
To evaluate the protective effect of CH on LPS-induced acute lung injury. Results showed that CH significantly ameliorated LPS-induced pathological changes in the lung, reduced inflammatory cell infiltration, and inhibited the production of TNF-α, IL-6, and IL-1β.
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