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Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Narciclasine triggers activation of Rho and stress fibers within glioblastoma multiforme (GBM) cells, exhibiting a mean IC50 of approximately 50 nM across six human GBM cell lines (U373, Hs683, GL19, GL5, GL16, GL17), which also demonstrates a mean IC50 value of 47 nM against a diverse array of 60 cancer cell lines[1]. Further exploration through bioassay-guided fractionation of A. belladonna extract pinpointed lycorine as the active compound, with Narciclasine showing a significant IC50 of 0.1 μM for radicle growth inhibition[2].
体内研究
In vivo studies reveal that an intravenous (i.v.) administration of Narciclasine at 1 mg/kg significantly enhances the survival rates of mice bearing GL19 GBM (P=0.02). Similarly, oral administration of Narciclasine at the same dosage, five times weekly for five weeks, markedly prolongs survival in this model (P=0.008). Oral treatment at 1 mg/kg also significantly boosts survival (P=0.004) in mice with Hs683 GBM, with no added survival benefit from increasing the dosage frequency. Narciclasine showcases efficacy comparable to that of temozolomide in these models, but at significantly lower doses and through both oral and i.v. routes[1].
体外研究
Narciclasine triggers activation of Rho and stress fibers within glioblastoma multiforme (GBM) cells, exhibiting a mean IC50 of approximately 50 nM across six human GBM cell lines (U373, Hs683, GL19, GL5, GL16, GL17), which also demonstrates a mean IC50 value of 47 nM against a diverse array of 60 cancer cell lines[1].
Further exploration through bioassay-guided fractionation of A. belladonna extract pinpointed lycorine as the active compound, with Narciclasine showing a significant IC50 of 0.1 μM for radicle growth inhibition[2].
Narciclasine/水仙环素 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Yeast strain SAN006
0.1 mM
Assess cytotoxicity of sanguinarine
Genes Dis. 2023 Apr 13;11(5):100938.
MDA-MB-231 breast cancer cells
1 µM
24 hours
Induced DNA laddering
Neoplasia. 2007 Sep;9(9):766-76.
CoN
1.1 µM (IC50)
72 hours
Inhibited cell proliferation with IC50 of 1.1 μM
Biomolecules. 2022 Sep 9;12(9):1267.
DLD-1
0.030 µM (IC50)
72 hours
Inhibited cell proliferation with IC50 of 0.030 μM
Biomolecules. 2022 Sep 9;12(9):1267.
HCT-116
0.052 µM (IC50)
72 hours
Inhibited cell proliferation with IC50 of 0.052 μM
Biomolecules. 2022 Sep 9;12(9):1267.
HT-29
0.032 µM (IC50)
72 hours
Inhibited cell proliferation with IC50 of 0.032 μM
Biomolecules. 2022 Sep 9;12(9):1267.
LoVo
0.023 µM (IC50)
72 hours
Inhibited cell proliferation with IC50 of 0.023 μM
Biomolecules. 2022 Sep 9;12(9):1267.
Ccd-25-Lu normal lung fibroblasts
1 µM
18 hours
No marked apoptotic morphological changes induced
Neoplasia. 2007 Sep;9(9):766-76.
PC-3 prostate cancer cells
1 µM
18 hours
Induced marked apoptotic morphological changes and loss of mitochondrial membrane potential
Neoplasia. 2007 Sep;9(9):766-76.
MCF-7 breast cancer cells
1 µM
18 hours
Induced marked apoptotic morphological changes and loss of mitochondrial membrane potential
Neoplasia. 2007 Sep;9(9):766-76.
Tobacco BY-2 cells
0.5 and 5.0 µM
2 days
To study the effects of NCS on cell cycle progression, results showed that NCS inhibits cell division and affects the expression of cell cycle genes.
BMC Plant Biol. 2011 Dec 28;11:184.
HCC-1937
50 nM
24 hours
To evaluate the inhibitory effect of Narciclasine on HCC-1937 cell proliferation and its ability to induce autophagy-dependent apoptosis
Cell Prolif. 2018 Dec;51(6):e12518.
MCF-7/TR cells
100 nM
24 hours
Specifically degraded total STAT3 via the proteasome pathway
Mol Ther Oncolytics. 2022 Jan 3;24:340-354.
MCF-7 cells
100 nM
24 hours
Suppressed phosphorylation, activation, dimerization, and nuclear translocation of STAT3
Mol Ther Oncolytics. 2022 Jan 3;24:340-354.
IMG cells
50 nM
24 hours
To evaluate the effect of Narciclasine on LPS-induced inflammatory response. Results showed that Narciclasine alone did not induce inflammatory response but exacerbated inflammation when combined with LPS.
Cells. 2023 May 11;12(10):1367.
BSC-1 cells
40-320 nM
24 hours
To evaluate the inhibitory effect of Narciclasine on VACV viral protein expression in BSC-1 cells, showing a dose-dependent decrease in viral protein levels.
Biosaf Health. 2024 Nov 19;6(6):341-349.
Huh7.5.1 cells
40-320 nM
24 hours
To evaluate the cytotoxicity of Narciclasine on Huh7.5.1 cells, showing a slight reduction in cell viability starting from 80 nM.
Biosaf Health. 2024 Nov 19;6(6):341-349.
HeLa cells
40-320 nM
24 hours
To evaluate the cytotoxicity of Narciclasine on HeLa cells, showing no significant effect on cell viability at ≤320 nM.
To analyze the effects of NCS on the expression of core cell cycle genes, results showed that NCS disturbs the expression of G1/S and G2/M phase genes.
BMC Plant Biol. 2011 Dec 28;11:184.
Neonatal rat cardiomyocytes (NRCMs)
0, 30, 100, and 300 nM
To evaluate the effect of Narciclasine on LPS-induced inflammation in cardiomyocytes. Results showed that Narciclasine attenuated LPS-induced decrease in cell viability in a dose-dependent manner, suppressed inflammatory cytokine (TNF-α, IL-1β, and IL-6) release, and reduced apoptosis.
Aging (Albany NY). 2021 May 25;13(11):15151-15163.
Narciclasine/水仙环素 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6 mice
LPS-induced acute myocardial injury model
Gavage
0.1 mg/kg
Once daily for 7 days
To investigate the protective effect of Narciclasine on LPS-induced myocardial injury. Results showed that Narciclasine partly improved cardiac function parameters (e.g., LVEF and LVFS), increased survival rates, and reduced inflammatory indicators (e.g., MPO and cytokines IL-6, IL-1β, TNF-α, VEGF).
Aging (Albany NY). 2021 May 25;13(11):15151-15163.
Neonatal rats
E. coli-induced sepsis model
Intraperitoneal injection
0.1 mg/kg, 1 mg/kg, 3 mg/kg
Administered 1 hour after sepsis induction, observed for 30 hours
Narciclasine significantly reduced mortality in neonatal rats with sepsis, alleviated clinical sepsis symptoms, reduced bacterial load, decreased levels of inflammatory cytokines, inhibited the expression of S100A8/A9, alleviated inflammation and injury in liver and lungs, improved hematological parameters, and suppressed the activation of the NF-κB signaling pathway.
Sci Rep. 2020 Feb 19;10(1):2947
Arabidopsis
Arabidopsis seedlings
Medium treatment
0.1, 0.5, 1.0 and 5.0 μM
Continuous treatment for 7 days
To study the inhibitory effects of NCS on Arabidopsis root growth, results showed that NCS significantly inhibited root growth and lateral root density.
BMC Plant Biol. 2011 Dec 28;11:184.
Sprague-Dawley rats
LPS-induced neonatal acute lung injury model
Subcutaneous injection
0.7 mg/kg
Administered 12 hours prior to LPS exposure, lasting for 72 hours
Narciclasine attenuated LPS-induced pathological injury and pulmonary edema, suppressed the secretion of inflammatory cytokines, alleviated oxidative stress and apoptosis, and exerted protective effects by inhibiting the activation of the TLR4/NF-κB/Cox2 signaling pathway.
Bioengineered. 2020 Dec;11(1):801-810
Mice
High-fat diet-induced obesity model
Oral gavage
1 mg/kg
Weekly for seven consecutive weeks
Attenuates obesity, promotes energy expenditure, improves metabolic parameters
PLoS Biol. 2017 Feb 16;15(2):e1002597.
BALB/c-nu mice
MCF-7 xenograft model
Intraperitoneal injection
1 mg/kg
Every 2 days for 4 weeks
Significantly reduced tumor size and weight, decreased STAT3 phosphorylation levels
Mol Ther Oncolytics. 2022 Jan 3;24:340-354.
BALB/c nude mice
HCC1937 TNBC xenograft model
Oral
2 or 5 mg/kg
Three times per week for 21 days
To evaluate the inhibitory effect of Narciclasine on HCC1937 TNBC xenograft tumor growth and its mechanism
Cell Prolif. 2018 Dec;51(6):e12518.
BALB/C mice
IMQ-induced psoriasis-like dermatitis model
Intradermal injection
2mg/kg
Once daily for 6 days
To evaluate the therapeutic effect of Ncs on IMQ-induced psoriasis-like dermatitis, results showed that Ncs significantly improved skin lesions, reduced epidermal thickening and immune cell infiltration.
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