There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
ZCL278 is a potent, cell-permeable inhibitor specific to Cdc42, suppressing actin-based cellular functions such as Golgi organization and cell motility. In Swiss 3T3 fibroblast cultures, ZCL278 disrupts microspike formation and GM130-docked Golgi structures, prominent Cdc42-mediated subcellular events. It reduces perinuclear accumulation of active Cdc42 compared to NSC23766, a selective Rac inhibitor. ZCL278 also inhibits Cdc42-mediated neuronal branching, growth cone dynamics, actin-based motility, and migration in PC-3 metastatic prostate cancer cells without impacting cell viability [1]. ZCL278 acts as an entry inhibitor for Junin virus (JUNV), vesicular stomatitis virus, lymphocytic choriomeningitis virus, and dengue virus, but not for nonenveloped poliovirus. It efficiently inhibits chemically induced filopodium formation, a process dependent on Cdc42 activity. Dose-response experiments in Vero cells show that ZCL278, up to 200 μM, is not toxic but inhibits JUNV with an IC50 of ~14 μM, measured by flow cytometry [2].
体内研究
ZCL278 decreases JUNV RNA load in the spleen over 33-fold, with JUNV RNA undetectable in 5 out of 8 mice, similar to Gabapentin-treated mice, indicating ZCL278's ability to halt JUNV replication [2].
体外研究
ZCL278 is a potent, cell-permeable inhibitor specific to Cdc42, suppressing actin-based cellular functions such as Golgi organization and cell motility. In Swiss 3T3 fibroblast cultures, ZCL278 disrupts microspike formation and GM130-docked Golgi structures, prominent Cdc42-mediated subcellular events. It reduces perinuclear accumulation of active Cdc42 compared to NSC23766, a selective Rac inhibitor. ZCL278 also inhibits Cdc42-mediated neuronal branching, growth cone dynamics, actin-based motility, and migration in PC-3 metastatic prostate cancer cells without impacting cell viability [1].
ZCL278 acts as an entry inhibitor for Junin virus (JUNV), vesicular stomatitis virus, lymphocytic choriomeningitis virus, and dengue virus, but not for nonenveloped poliovirus. It efficiently inhibits chemically induced filopodium formation, a process dependent on Cdc42 activity. Dose-response experiments in Vero cells show that ZCL278, up to 200 μM, is not toxic but inhibits JUNV with an IC50 of ~14 μM, measured by flow cytometry [2].
作用机制
ZCL278 is a small molecule that binds to Cdc42 residues to target the interaction between Cdc42 and intersectin and inhibits Cdc42-mediated cellular processes.
ZCL278 细胞实验
Cell Line
Concentration
Treated Time
Description
References
PC3 prostate cancer cells
20 µM
48, 96, 144 hours
Inhibited cell proliferation
Cancer Biol Ther. 2019;20(6):740-749.
A549 lung cancer cells
50 µM
48, 96, 144 hours
Inhibited cell proliferation
Cancer Biol Ther. 2019;20(6):740-749.
Swiss 3T3 cells
0.01, 0.1, 1, 10, 50, 100 µM
15 minutes
ZCL278 promoted Cdc42 activation
Commun Chem. 2024 Nov 19;7(1):271.
Swiss 3T3 cells
50 µM
2 hours
Activated Cdc42 and promoted microspike formation
Cancer Biol Ther. 2019;20(6):740-749.
LNCaP prostate cancer cells
20 µM
48, 96, 144 hours
Inhibited cell proliferation
Cancer Biol Ther. 2019;20(6):740-749.
Capan-1
1, 5, and 20 µM
10 minutes
To assess the inhibitory effect of ZCL278 on Cdc42 function, results showed that ZCL278 decreased Cdc42 activity in a dose-dependent manner and inhibited microspike formation and stress fibers
Cell Prolif. 2020 Apr;53(4):e12799.
PANC-1
1, 5, and 20 µM
10 minutes
To assess the inhibitory effect of ZCL278 on Cdc42 function, results showed that ZCL278 decreased Cdc42 activity in a dose-dependent manner and inhibited microspike formation and stress fibers
Cell Prolif. 2020 Apr;53(4):e12799.
Swiss 3T3 cells
50 µM
10, 20, 30, 60, 120, 360 minutes
ZCL278 at 50 µM formed an initial peak at 10 minutes. ZCL278 then displayed an antagonistic effect at 30 minutes followed by resuming a strong agonistic effect up to 6 hours without showing the plateau
Commun Chem. 2024 Nov 19;7(1):271.
Swiss 3T3 fibroblasts
50 µM
15 min or 1 hour
Inhibited Cdc42-mediated microspike formation without affecting RhoA-mediated stress fibers
Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1261-6.
SVG-A cells
50 µM
30 minutes
ZCL278 inhibited JUNV infection without affecting cell viability.
J Virol. 2016 Apr 14;90(9):4494-4510.
Vero cells
50 µM
30 minutes
ZCL278 inhibited JUNV infection with an IC50 of ∼14 μM and showed no cytotoxicity.
J Virol. 2016 Apr 14;90(9):4494-4510.
Primary neonatal cortical neurons
50 µM
5 and 10 min
Inhibited neuronal branching and growth cone dynamics
Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1261-6.
PC-3 prostate cancer cells
50 µM
5, 10, and 15 min
Inhibited Cdc42 activity and increased Rac/Cdc42 phosphorylation
Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1261-6.
Human Umbilical Cord Sca-1+ Progenitor Cells (HUCSCs)
搜索
HazMat Fee +
