Hypericin, a powerful naturally occurring photosensitizer, is found in Hypericum perforatum plants, commonly known as St. John's wort. Hypericin can induce both apoptosis and necrosis in a concentration and light dose-dependent fashion[3]. Among its antidepressant and light-dependent antiviral actions, hypericin is a powerful natural photosensitizer that is applicable in the photodynamic therapy (PDT) of various oncological diseases. In addition, light-activated hypericin acts as a strong pro-oxidant agent with antineoplastic and antiangiogenic properties, since it effectively induces the apoptosis, necrosis or autophagy of cancer cells. Moreover, a strong affinity of hypericin for necrotic tissue was discovered[4]. Hypericin significantly decreased the minimum inhibitory concentrations of β-lactam antibiotics (e.g., oxacillin, cefazolin and nafcillin), biofilm formation and fibronectin binding in MRSA strain JE2[5]. In vivo, prophylactic or therapeutic use of hypericin inhibited islet β-cell apoptosis and enhanced the anti-oxidative ability of pancreatic tissue in high-fat/high-sucrose (HFHS)-fed mice, thus alleviating β-cell loss and maintaining or improving β-cell mass and islet size. More importantly, hypericin treatment decreased fasting blood glucose, improved glucose intolerance and insulin intolerance, and alleviated HFHS-fed mice[6].
To investigate the effect of hypericin on the minimum inhibitory concentrations (MICs) of β-lactam antibiotics (e.g., oxacillin, cefazolin, and nafcillin), showing that hypericin significantly decreased the MICs of these antibiotics.
Acta Pharm Sin B. 2019 Nov;9(6):1174-1182
Vero E6 cells
3, 30, 300 nM
15 minutes under light or dark conditions
To evaluate the antiviral efficacy of Hypericin against SARS-CoV-2. Results showed that under light conditions, 30 and 300 nM Hypericin significantly reduced viral titer (TCID50/mL from 105.5 to <101); under dark conditions, 300 nM Hypericin also significantly reduced viral titer (from 105.5 to 102.5).
ACS Appl Mater Interfaces. 2022 Mar 30;14(12):14025-14032
INS-1 cells
20 nM, 200 nM, 2000 nM
72 hours
To investigate the protective effect of Hypericin on INS-1 cell viability under high-glucose conditions. Results showed that Hypericin significantly improved INS-1 cell viability under high-glucose conditions, inhibited high-glucose-induced apoptosis and NO production.
Int J Biol Sci. 2019 Jun 2;15(7):1472-1487
HepG2 cells
50-500 nM
2 hours
To evaluate the transfection efficiency of photochemical internalization (PCI) technology in HepG2 cells. Results showed that transfection efficiency was significantly enhanced at 600 mJ/cm² radiant exposure, with a 60-75 fold increase compared to nonirradiated cells.
ACS Appl Mater Interfaces. 2024 Aug 21;16(33):43416-43429
Hypericin/金丝桃素 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Alcoholic liver injury model
Gavage
168.50 mg/g
Once daily for 8 weeks
Hyperoside prevents alcoholic liver injury through antioxidation, with effects equivalent to silymarin
Once daily (hypericin), Three times a day (oxacillin), for 3 days
To evaluate the therapeutic effect of hypericin in combination with oxacillin in the MRSA bacteremia model, showing that the combination significantly reduced MRSA densities in target tissues.
Acta Pharm Sin B. 2019 Nov;9(6):1174-1182
C57BL/6J mice
HFHS-induced type II diabetes model
Intraperitoneal injection
0.5 mg/kg, 1 mg/kg, 2 mg/kg
Administered every other day for four months (prevention group) or 30 days (treatment group)
To investigate the preventive and therapeutic effects of Hypericin on HFHS-induced type II diabetes in mice. Results showed that Hypericin significantly reduced fasting blood glucose levels, improved glucose intolerance and insulin intolerance, decreased islet β-cell apoptosis, and enhanced the anti-oxidative ability of pancreatic tissue.
United States, Maryland
... 展开 >>
Johns Hopkins Adult AIDS CRS
Baltimore, Maryland, United States, 21287
United States, Massachusetts
Beth Israel Deaconess - East Campus A0102 CRS
Boston, Massachusetts, United States, 02215
United States, New York
NY Univ. HIV/AIDS CRS
New York, New York, United States, 10016 收起 <<
United States, Maryland
... 展开 >>
Johns Hopkins Adult AIDS CRS
Baltimore, Maryland, United States, 21287
United States, Massachusetts
Beth Israel Deaconess - East Campus A0102 CRS
Boston, Massachusetts, United States, 02215
United States, New York
NY Univ. HIV/AIDS CRS
New York, New York, United States, 10016 收起 <<
Germany
... 展开 >> University Department of General, Visceral and Transplant Surgery
Recruiting
Tübingen, Baden.Württemberg, Germany, 72076
Contact: Stefan Beckert, Prof. Dr. +49-(0)7071-29-86619 stefan.beckert@med.uni-tuebingen.de
Contact: Alfred Königsrainer, Prof. Dr. +49-(0)7071-29-86619 alfred.koenigsrainer@med.uni-tuebingen.de 收起 <<
United States, Massachusetts
... 展开 >>
Beth Israel Deaconess - East Campus A0102 CRS
Boston, Massachusetts, United States, 02215
United States, Minnesota
University of Minnesota, ACTU
Minneapolis, Minnesota, United States
United States, New York
NY Univ. HIV/AIDS CRS
New York, New York, United States, 10016 收起 <<
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