EIPA significantly inhibited LCMV multiplication in 293T cells
J Virol. 2014 Jan;88(1):643-54.
TZM-bl cells
50 µM
30 minutes
Inhibition of HIV-1 infection
ACS Nano. 2023 Sep 12;17(17):17436-17450.
RAS3 cells
50 µM
1 hour
Inhibited EV uptake via macropinocytosis in RAS3 cells
J Extracell Vesicles. 2021 Jun;10(8):e12091.
HSC-4 cells
25 µM
24 hours
Inhibited the uptake of HSC-4-derived exosomes by HSC-4 cells
Cancer Sci. 2022 Feb;113(2):609-621.
IEC18 cells
50 µM
1 hour
No significant effect on EV uptake in non-transformed cells
J Extracell Vesicles. 2021 Jun;10(8):e12091.
HTcEpi cells
20 µM
2 hours
EIPA significantly reduced belantamab-specific cell killing from 34.5% to 15.5%.
Haematologica. 2024 Nov 1;109(11):3670-3680.
MDA-MB-231RR radioresistant cells
20 µM or 50 µM
24 hours
EIPA treatment reversed the transcriptomic changes in radioresistant cells, downregulating NF-κB, senescence, and extracellular matrix-related genes.
Neoplasia. 2023 Jan;35:100862.
A549 cells
10 µM
30 min pretreatment
EIPA strongly inhibited LCMV multiplication in A549 cells
J Virol. 2014 Jan;88(1):643-54.
BHK-21 cells
5-10 µM
30 min pretreatment
EIPA strongly inhibited LCMV multiplication in BHK-21 cells
J Virol. 2014 Jan;88(1):643-54.
MHCC97L cells
35 or 75 µM
30 minutes
To evaluate the effect of EIPA on macropinocytosis and sEV uptake. The results showed that EIPA significantly reduced the uptake of TMR-dextran and PKH67-sEVs.
Cancer Commun (Lond). 2024 Feb;44(2):251-272.
MHCCLM3 cells
35 or 75 µM
30 minutes
To evaluate the effect of EIPA on macropinocytosis and sEV uptake. The results showed that EIPA significantly reduced the uptake of TMR-dextran and PKH67-sEVs.
Cancer Commun (Lond). 2024 Feb;44(2):251-272.
Mouse skin fibroblasts (MSF)
10 µM
30 seconds
To detect the inhibitory effect of EIPA on NHE, results showed a significant response to EIPA in NHE3-reconstituted MSF cells, while NHE-deficient MSF cells showed no response.
Biosensors (Basel). 2016 Mar 30;6(2):11.
Chinese hamster ovary (CHO) cells
10 µM
30 seconds
To detect the inhibitory effect of EIPA on NHE, results showed a significant response to EIPA inhibition in CHO cells.
Biosensors (Basel). 2016 Mar 30;6(2):11.
AsPC-1 human pancreatic cancer cells
5 µM
48 hours
EIPA significantly decreased the clonogenic survival of AsPC-1 cells, indicating a radiosensitizing effect.
Neoplasia. 2023 Jan;35:100862.
MDA-MB-231 human breast cancer cells
5 µM
48 hours
EIPA significantly decreased the plating efficiency of MDA-MB-231 cells from 52% to 35%, indicating a radiosensitizing effect.
Neoplasia. 2023 Jan;35:100862.
U373-MG cells
25 µM
48 or 72 hours
EIPA significantly increased the autophagic response induced by trehalose, and the effect of combined molecules appeared to be synergistic at 48 and 72 hours of treatment.
Cancer Cell Int. 2022 Jul 21;22(1):232.
Human neutrophils
75 µM
5 hours
EIPA triggers NETotic cell death accompanied by an increase in intracellular ROS
Redox Biol. 2021 Jul;43:101983.
Rat glomerular mesangial cells (GMC)
10 µM
EIPA completely prevented aldosterone-induced alkalinization by inhibiting Na+/H+ exchange.
Biomedicines. 2021 Aug 30;9(9):1117.
EIPA 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
BALB/c nude mice
MDA-MB-231 xenograft tumor model
Intraperitoneal injection
12 mg/kg
Three times a week for the first two weeks, then twice a week for the next two weeks, lasting four weeks
Combination therapy of EIPA and radiation significantly inhibited the growth of MDA-MB-231 xenograft tumors, increased apoptotic cells in tumor tissues, and reduced the expression of NHE1 and phospho-NF-κB p65.
Neoplasia. 2023 Jan;35:100862.
BALB/cAnN-nu mice
Subcutaneous xenograft model
Subcutaneous injection
5 mg/kg
Single injection
To evaluate the effect of EIPA on sEV-induced tumor growth. The results showed that EIPA significantly inhibited sEV-induced tumor growth.
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