Benzocaine is the ethyl ester of p-aminobenzoic acid (PABA), it is a local anesthetic commonly used as a topical pain reliever or in cough drops.
Benzocaine/苯佐卡因 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Zebrafish (Danio rerio)
Zebrafish embryos and larvae
Immersion
1 g/L
1 hour
To evaluate the efficacy of benzocaine as a method of euthanasia for zebrafish embryos and larvae. Results showed benzocaine was almost 100% effective in 4 dpf larvae but failed to induce irreversible death in 24 hpf embryos.
Biology (Basel). 2022 Apr 1;11(4):546
Nile Tilapia
Anesthesia and stunning experiments
Water immersion
40 mg/L and 80 mg/L
Single treatment until loss and recovery of visual sensibility indicators
To verify whether the presence/absence of a dorsal fin erection (DFE) response can be used as a painless VSI in Nile tilapia. All fish stunned using benzocaine and ice water immersion lose both VSIs, while 95% of fish stunned using CO2 lose these VSIs. In all treatments, DFEs return quicker than VOR.
Animals (Basel). 2021 Oct 19;11(10):3007
Arabidopsis
Arabidopsis wild type
Leaf infiltration
100 μM
Single treatment, pathogen inoculation five hours later
To investigate the mechanisms underlying BEN’s activation of local plant defense in Arabidopsis. Results showed that BEN significantly enhanced local resistance in wild-type plants, but its effect was notably subdued in SA or JA mutants.
Plants (Basel). 2024 Feb 28;13(5):672
Atlantic salmon
Atlantic salmon
Water exposure
200 mg/L
Single dose
To study the effect of benzocaine on the metabolome of salmon skin and gill mucus, finding that benzocaine treatment led to disturbances in amino acid metabolic pathways.
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