There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
10-Hydroxycamptothecin (HCPT) is an inhibitor of topoisomerase I and can affect phosphorylation of histone H1 and H3 with anticancer activity. It can be isolated from camptotheca acuminate. Gel electrophoresis found that HCPT had a selectively inhibitory effect on the phosphorylation of histone H1 and H3, but less effect on the other kinds of histones. In vivo, HCPT also exhibited a suppressive effect on histone H1 and H3 phosphorylation[1]. After treatment with HCPT at differentiation-inducing concentrations 5-20 mg/L for 6 d, Hep G2 cells were mainly arrested at G2/M phase and the PCNA expression rate was lower than that of control cells. When Hep G2 cells grew in a medium containing HCPT 5 mg/L for 6 d, the p53 expression level markedly increased in comparison with the control cells. The telomerase activity did not change in Hep G2 cells treated with HCPT 5-20 mg/L for 8 d[2]. HCPT exhibited adverse effects on mouse oocyte maturation and quality, and MT (Melatonin) administration alleviated the negative influence of HCPT[3].
(±)-10-Hydroxycamptothecin/10-羟基喜树碱 细胞实验
Cell Line
Concentration
Treated Time
Description
References
LO2 cells
100 μM
48 h
Evaluate the cytotoxicity of HpYss on normal LO2 cells, with an IC50 significantly lower than free HCPT.
Bioact Mater. 2021 Jul 24;9:120-133.
A549 cells
100 μM
48 h
Evaluate the cytotoxicity of HpYss on A549 cells, with an IC50 similar to free HCPT.
Bioact Mater. 2021 Jul 24;9:120-133.
PC-3 cells
100 μM
48 h
Evaluate the cytotoxicity of HpYss on PC-3 cells, with an IC50 similar to free HCPT.
Bioact Mater. 2021 Jul 24;9:120-133.
HeLa cells
100 μM
48 h
Evaluate the cytotoxicity of HpYss on HeLa cells, with an IC50 significantly lower than free HCPT.
Bioact Mater. 2021 Jul 24;9:120-133.
MCF-7 cells
100 μM
48 h
Evaluate the cytotoxicity of HpYss on MCF-7 cells, with an IC50 significantly lower than free HCPT.
Bioact Mater. 2021 Jul 24;9:120-133.
HepG2 cells
100 μM
48 h
Evaluate the cytotoxicity of HpYss on HepG2 cells, with an IC50 of 80 nM, significantly lower than other control groups.
Bioact Mater. 2021 Jul 24;9:120-133.
(±)-10-Hydroxycamptothecin/10-羟基喜树碱 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
BALB/c nude mice
HepG2 xenograft model
Intravenous injection
3 mg/kg HCPT-equivalent
Once every three days, total three times
Evaluate the antitumor efficacy of HpYss in HepG2 xenograft model, significantly inhibiting tumor growth.
Bioact Mater. 2021 Jul 24;9:120-133.
BALB/c nude mice
Orthotopic glioma model
Tail intravenous injection
5 mg/kg HCPT
Administered at Days 7, 9, 12, 15, 18 and 21
To evaluate the anti-glioma effect of HCPT-M-CA-NPs in the glioma model, the results showed that the median survival time of the HCPT-M-CA-NPs group (25.5 days) was significantly longer than that of the free HCPT group (17.0 days) and the HCPT-CA-NPs group (22.0 days).
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