Betulinic Acid is a natural pentacyclic triterpenoid compound and a eukaryotic topoisomerase I inhibitor with IC50 value of 0.5 mM. It has anti-inflammatory, anti malaria, anti AIDS and anti-tumor activities[3]. Betulinic Acid is also a new activator of NF κB[4]. Betulinic Acid (20, 40 µM) causes decrease in Bcl-2 expression of MDA-MB-231 cells[5]. Betulinic Acid has anti HIV activity. EC50 of H9 lymphocytes infected with Betulinic Acid is 1.4 μ M[6]. Moreover, oral administration of Betulinic Acid reduced the bleeding score at 10 and 30 mg/kg and improved the stool consistency at 30 mg/kg[7].
Betulinic acid/白桦脂酸 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HT22 cells
12 µg/mL
BA showed no significant toxicity to HT22 cells
J Nanobiotechnology. 2022 Jan 21;20(1):39.
PANC-1 cells
5, 10, 20 μM
12, 24, 48 hours
To assess the direct cytotoxicity of BA, which showed concentration- and time-dependent inhibition of cell proliferation.
Cancer Res. 2011 Aug 1;71(15):5182-93.
FG cells
2.5, 5, 10 μM
48 hours
To evaluate the cytotoxic effects of BA and MIT alone or in combination, showing inhibition of cell proliferation.
Cancer Res. 2011 Aug 1;71(15):5182-93.
BxPC-3 cells
5, 10, 20 μM
12, 24, 48 hours
To assess the direct cytotoxicity of BA, which showed concentration- and time-dependent inhibition of cell proliferation.
Cancer Res. 2011 Aug 1;71(15):5182-93.
A172 cells
0, 3, 6, 12, 24, 48 µg/mL
24 hours
BA NPs significantly inhibited A172 cell proliferation with IC50 of 8.5 µg/mL
J Nanobiotechnology. 2022 Jan 21;20(1):39.
U87 cells
0, 3, 6, 12, 24, 48 µg/mL
24 hours
BA NPs significantly inhibited U87 cell proliferation with IC50 of 12.1 µg/mL
J Nanobiotechnology. 2022 Jan 21;20(1):39.
PANC-1 cells
50 μM
48 hours
Gene expression profiles were analyzed by cDNA microarray after BA treatment, revealing significant downregulation of lamin B1.
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