Neobavaisoflavone (NBIF), a flavonoid, is isolated from the seeds of Psoralea corylifolia. Neobavaisoflavone exhibits anti-inflammatory, anti-cancer and anti-oxidation activities. Neobavaisoflavone significantly inhibited the production of reactive oxygen species (ROS), reactive nitrogen species (RNS) and cytokines: IL-1β, IL-6, IL-12p40, IL-12p70, TNF-α in LPS+IFN-γ- or PMA- stimulated RAW264.7 macrophages[2]. NBIF is a natural inducer of UGT1A1(UDP-glucuronosyltransferase 1A1), while this agent induced UGT1A1 mainly via activating and up-regulating PPARα and PPARγ[3]. NBIF inhibited RANKL-mediated osteoclastogenesis by suppressing the recruitment of TRAF6 and c-Src to RANK, inactivating NF-κB, MAPKs, and Akt signalling pathways and inhibiting calcium oscillation and NFATc1 (nuclear factor of activated T cells cytoplasmic 1) translocation[4]. NBI (neobavaisoflavone) inhibited melanin production through the regulation of MEK/ERK and Akt/GSK-3β signaling pathways in α-MSH-stimulated B16F10 cells. NBI suppresses tyrosinase activity and melanogenesis through inhibition of α-glucosidase activity. Besides, NBI significantly reduced melanogenesis in a reconstructed human 3D skin model[5].
Neobavaisoflavone/新补骨脂异黄酮 细胞实验
Cell Line
Concentration
Treated Time
Description
References
BV-2 microglia cells
50 μM
24 h
Inhibition of LPS-induced NO production
Molecules. 2016 Aug 17;21(8):1076.
RAW264.7 macrophages
1–100 μM
20 h
Neobavaisoflavone significantly inhibited the production of ROS, RNS, and cytokines (IL-1β, IL-6, IL-12p40, IL-12p70, TNF-α) in LPS+IFN-γ or PMA-stimulated RAW264.7 macrophages
Molecules. 2011 May 3;16(5):3701-12.
MC3T3-E1 cells
0.01, 0.05, 0.25, 1 mM
1, 3, 7 days
To evaluate the cytocompatibility of hydrogels with MC3T3-E1 cells, results showed that the hydrogels were non-toxic to the cells and supported good cell growth.
Asian J Pharm Sci. 2025 Feb;20(1):100988.
rat liver microsome
0.1 mg/mL
5, 10, 15, 30, 45, 60, 120, and 240 min
To study the metabolites of Neobavaisoflavone in rat liver microsome
Molecules. 2022 Dec 1;27(23):8413.
U-87 MG glioblastoma cells
25 µM
48 h
To investigate the effect of NBIF in combination with doxorubicin or etoposide on apoptosis, results showed that NBIF enhanced the pro-apoptotic activity of both drugs.
Int J Mol Sci. 2022 May 17;23(10):5621.
Normal human astrocytes
25 µM
48 h
To investigate the effect of NBIF in combination with doxorubicin or etoposide on apoptosis, results showed that NBIF enhanced the pro-apoptotic activity of both drugs.
Int J Mol Sci. 2022 May 17;23(10):5621.
primary hepatocytes
0.2 μg/ml
24 h
Neobavaisoflavone ameliorates hepatocyte injury, relieves oxidative stress, and downregulates the PKC-α/NOX signaling pathway.
Attenuate Palmitic Acid-Induced Hepatocyte Injury via Inhibiting the Protein Kinase C-α/Nicotinamide-Adenine Dinucleotide Phosphate Oxidase Pathway. Front Pharmacol.
Neobavaisoflavone/新补骨脂异黄酮 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Rats
Normal rats
Oral
150 mg/kg
3 consecutive days
To study the metabolites of Neobavaisoflavone in rats
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