Harmine is a beta-carboline alkaloid found in medicinal plant Peganum Harmala, which has served as a folk anticancer medicine. Harmine decreased the survival of HepG2 Cells and the cell survival rates were decreased with increasing the compounds concentrations (from 0 to 10 μM). In vivo,the mice receiving 100 mg/kg harmine exhibited neurotoxic behaviors immediately[3].In addition, harmine administration was associated with neuroprotective effects and also improved memory/learning in several animal models[4].
Harmine/去氢骆驼蓬碱 细胞实验
Cell Line
Concentration
Treated Time
Description
References
R7T1 mouse insulinoma cells
10 µM
24 hours
To evaluate the effect of Harmine and its analogs on NFAT2-GFP nuclear translocation, the results showed that some analogs significantly increased NFAT2-GFP nuclear translocation at 10 μM concentration.
J Med Chem. 2020 Mar 26;63(6):2986-3003.
B16-F10 melanoma cells
5 and 10 µM
24 hours
ACB1801 upregulates the mRNA expression of genes involved in MHC-I antigen presentation, such as TAP1, TAP2, Tapasin, and Lmp2.
Front Immunol. 2022 Nov 15;13:980704.
CT26 colorectal cancer cells
10 µM
24 hours
ACB1801 upregulates the mRNA expression of TAP1, TAP2, and Tapasin.
Front Immunol. 2022 Nov 15;13:980704.
A375 melanoma cells
5 and 10 µM
24 hours
ACB1801 upregulates the mRNA and protein expression of TAP1.
Front Immunol. 2022 Nov 15;13:980704.
HCT116 colorectal cancer cells
5 and 10 µM
24 hours
ACB1801 upregulates the mRNA and protein expression of TAP1.
Front Immunol. 2022 Nov 15;13:980704.
U87 and U251 glioblastoma cells
5 and 10 µM
24 hours
ACB1801 upregulates the mRNA and protein expression of TAP1.
Front Immunol. 2022 Nov 15;13:980704.
Spodoptera frugiperda Sf9 cells
5, 10, 25, 50, 100 μg/mL
24, 48 hours
Harmine and ZC-14 inhibited the proliferation of Sf9 cells in a dose-dependent manner, and ZC-14 exhibited higher cytotoxicity than Harmine.
Int J Mol Sci. 2018 Mar 11;19(3):811.
SH-SY5Y cells
0.1 to 1 µM
48 hours
To evaluate the toxicity of compounds 13 and 17d on SH-SY5Y cells. The results showed that compound 13 exhibited negligible cytotoxicity at concentrations ranging from 0.1 to 1 µM.
J Enzyme Inhib Med Chem. 2023 Dec;38(1):2281893.
SH-SY5Y cells
1, 5, and 10 µM
48 hours
To evaluate the neuroprotective effects of compounds 13 and 17d against Al 1−42-induced damage in SH-SY5Y cells. The results showed that compound 13 demonstrated exceptional cellular recovery even at the lowest concentration of 1 µM.
J Enzyme Inhib Med Chem. 2023 Dec;38(1):2281893.
Spodoptera frugiperda Sf9 cells
7.5 μg/mL
6, 12, 18, 24 hours
ZC-14 induced apoptosis in Sf9 cells, while Harmine did not show this effect at the same concentration.
Int J Mol Sci. 2018 Mar 11;19(3):811.
Human pancreatic islets
10 µM
96 hours
To evaluate the effect of Harmine and its analogs on the proliferation of human pancreatic islet β-cells, the results showed that some analogs significantly increased β-cell proliferation at 10 μM concentration.
J Med Chem. 2020 Mar 26;63(6):2986-3003.
Harmine/去氢骆驼蓬碱 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL6N mice
Diabetes model
Intraperitoneal injection
1 mg/kg, 3 mg/kg, 6 mg/kg, 10 mg/kg
Daily for 7 days
To evaluate the effect of Harmine and its analogs on the proliferation of mouse pancreatic islet β-cells, the results showed that some analogs significantly increased β-cell proliferation at 1 mg/kg and 3 mg/kg doses.
J Med Chem. 2020 Mar 26;63(6):2986-3003.
C57BL/6 mice
B16-F10 melanoma model
Oral
10 mg/kg
Once daily for 9 days
ACB1801 significantly inhibited the growth and weight of B16-F10 tumors and prolonged the survival of tumor-bearing mice. When combined with anti-PD-1, ACB1801 significantly enhanced the therapeutic efficacy of anti-PD-1.
Front Immunol. 2022 Nov 15;13:980704.
Mice
ΑDKRC::RLTG mice
Osmotic minipump
10 mg/kg/day
Two weeks
Harmine improved left ventricular ejection fraction (LVEF) and increased cardiomyocyte cycling
Austria
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Austria
... 展开 >> Department of Psychiatry and Psychotherapy, Medical University of Vienna
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Vienna, Austria, 1090
Contact: Rupert Lanzenberger, A/Prof. +43 40400 ext 3825 rupert.lanzenberger@meduniwien.ac.at
Principal Investigator: Rupert Lanzenberger, A/Prof. MD 收起 <<
Austria
... 展开 >> Department of Psychiatry and Psychotherapy
Recruiting
Vienna, Austria, 1090
Contact: Rupert Lanzenberger, Assoc. Prof. PD MD +43-1-40400-35760 rupert.lanzenberger@meduniwien.ac.at 收起 <<
Austria
... 展开 >> Department of Psychiatry and Psychotherapy, Medical University of Vienna
Recruiting
Vienna, Austria, 1090
Contact: Rupert Lanzenberger, A/Prof. +43 40400 ext 3825 rupert.lanzenberger@meduniwien.ac.at
Principal Investigator: Rupert Lanzenberger, A/Prof. MD 收起 <<
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