The dual-specificity tyrosine-phosphorylation-regulated (DYRK) kinases belong to a family collectively referred to as CMGC kinases that includes cyclin dependent kinases, mitogen associated protein kinases, glycogen synthase kinases, and cyclin dependent-like kinases. ID-8, a DYRK inhibitor, shows high specificity for DYRK1A and DYRK1B with IC50s of 78 and 54 nM, respectively[3]. ID-8 increased hESC (human embryonic stem cells) survival (∼1.1% at 0.5 μM). The combination of Wnt3a and ID-8 further increased survival (∼1.7%) and completely prevented Wnt-induced differentiation morphologically without disrupting proliferation. Wnt-induced differentiation marker gene expression was dramatically reduced by ID-8 at a dose that maintained the undifferentiated state[4]. Treatment with ID-8 (10 µM) promoted significant increases in cell number after 24 hours of damage in at least two different damage models[5].
ID-8 细胞实验
Cell Line
Concentration
Treated Time
Description
References
ID-8 cells
0, 1.2, 2.4, 4.8, 9.6 μg/mL
24 hours
To evaluate the effect of Tanshinone IIA on VEGF and COX2 mRNA expression in ID-8 cells, results showed that Tanshinone IIA significantly inhibited VEGF and COX2 mRNA expression.
Ann Transl Med. 2020 Oct;8(20):1295
ID-8 cells
0, 1.2, 2.4, 4.8, 9.6 μg/mL
24 hours
To evaluate the effect of Tanshinone IIA on the migration of ID-8 cells, results showed that Tanshinone IIA significantly inhibited cell migration.
Ann Transl Med. 2020 Oct;8(20):1295
ID-8 cells
0, 1.2, 2.4, 4.8, 9.6 μg/mL
24 hours
To evaluate the effect of Tanshinone IIA on the apoptosis of ID-8 cells, results showed that Tanshinone IIA significantly upregulated Bax mRNA expression and downregulated Bcl-2 mRNA expression, promoting apoptosis.
Ann Transl Med. 2020 Oct;8(20):1295
ID-8 cells
0, 1.2, 2.4, 4.8, 9.6 μg/mL
24 hours
To evaluate the effect of Tanshinone IIA on the proliferation of ID-8 cells, results showed that Tanshinone IIA significantly inhibited the proliferation of ID-8 cells in a dose-dependent manner.
Ann Transl Med. 2020 Oct;8(20):1295
ID-8 cells
1000 nM
6 hours (migration) and 16 hours (invasion)
To evaluate the effect of DEX on cell migration and invasion abilities, results showed that DEX significantly reduced the migration and invasion abilities of ID-8 cells, and this inhibition could be abrogated by depletion of endogenous miR-708
PLoS One. 2017 Jun 7;12(6):e0178937
ID-8 cells
100-1000 nM
48 hours
To evaluate the effect of DEX on Rap1B expression, results showed that DEX reduced Rap1B expression
PLoS One. 2017 Jun 7;12(6):e0178937
ID-8 cells
100-1000 nM
72 hours
To evaluate the effect of DEX on miR-708 expression, results showed that DEX significantly induced miR-708 expression
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