There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Blasticidin S inhibits protein synthesis by acting on the step of peptide transfer from peptidyl-sRNA to incoming aminoacyl-sRNA. Blasticidin S also significantly inhibits DNA synthesis independent of their effect on protein synthesis. Blasticidin S kills the cells more rapidly than G418, which makes blasticidin S-resistance gene (bsr) a selectable marker for mammalian cells. Besides, Blasticidin S also efficiently binds Cu(II) ions and prevents DNA from metal-induced damage.
Blasticidin S·HCl/杀稻瘟菌素盐酸盐 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HeLa cells
5 µg/ml to 100 µg/ml
3 hours
At sub-inhibitory concentrations (5 µg/ml and 10 µg/ml), BlaS increased protein expression from nonsense-mutated mRNA, while at higher concentrations (50 µg/ml and 100 µg/ml), BlaS significantly inhibited protein expression.
Nucleic Acids Res. 2021 Jul 21;49(13):7665-7679.
Rabbit Reticulocyte Lysate (RRL)
5 nM to 175 nM
30 minutes
BlaS inhibited translation at concentrations as low as 5 nM and completely inhibited translation at concentrations above 175 nM. The half-maximal inhibitory concentration (IC50) was determined to be 21 nM.
Nucleic Acids Res. 2021 Jul 21;49(13):7665-7679.
Plasmodium falciparum (FCB)
2.5 µg/ml
4 weeks
To select for blasticidin S-resistant Plasmodium falciparum mutants and study the functional changes in the PSAC channel.
Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1063-8.
Bacteria and eukaryotic cells
1 mM
To investigate the effect of BlaS on tRNA affinity to the 50S P site, it was found that BlaS significantly increased the fraction of nonrotated ribosomes and reduced the rate of tRNA transition from the classical P/P state to the hybrid P/E state.
Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12283-8.
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