Isovitexin, a natural product isolated and purified from the seeds of Vitex trifolia L., exhibits a potential antioxidant activity, shows a strong antihyperglycemic action, and inhibits α-glucosidase in vivo.
Isovitexin/异牡荆黄素 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Rat bone marrow mesenchymal stem cells (BMSCs)
150μmol/L
To validate the restorative effects of Isovitexin on osteogenic differentiation, results showed that Isovitexin restored mitochondrial function and inhibited ferroptosis by upregulating SIRT3 expression.
Bone Res. 2025 Jan 26;13(1):18
A549 cells
256 μg/ml
2 hours
To determine whether isovitexin could block S. aureus internalization into the lung epithelial cells. Results showed that isovitexin significantly decreased the amount of intracellular S. aureus.
J Microbiol Biotechnol. 2022 Oct 28;32(10):1284-1291
porcine ovarian granulosa cells (GCs)
40 mmol/L
12 hours
Promoted the proliferation of porcine GCs and enhanced the enzyme activity of UCHL1.
J Anim Sci Biotechnol. 2024 Jun 11;15(1):85
HuH7 cells
30 µM
72 hours
Evaluate the effect on cholesterol biosynthesis, showing a reduction in cholesterol biosynthesis (-5.7%, not statistically significant)
Antioxidants (Basel). 2021 Jun 29;10(7):1042
MC3T3-E1 cells
150 μmol/L
24 hours
To investigate the effects of Isovitexin on glucocorticoid-induced oxidative stress and osteonecrosis, results showed that Isovitexin effectively regulated mitophagy, preserved mitochondrial homeostasis, suppressed ferroptosis, and restored osteogenic capacity by promoting SIRT3 expression.
Bone Res. 2025 Jan 26;13(1):18
HPAECs (Human Pulmonary Artery Endothelial Cells)
25 or 50 μg/ml
24 hours
Isovitexin inhibited LPS-induced IL-6 production in HPAECs.
Int J Biol Sci. 2016 Jan 1;12(1):72-86
RAW 264.7 cells
25 or 50 μg/ml
24 hours
Isovitexin significantly reduced LPS-induced pro-inflammatory cytokine secretion, iNOS and COX-2 expression, and decreased ROS generation.
Int J Biol Sci. 2016 Jan 1;12(1):72-86
Isovitexin/异牡荆黄素 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
SD rats
Glucocorticoid-induced osteonecrosis of the femoral head model
Intraperitoneal injection
15 mg/kg
Every two days for 4 weeks
To validate the preventive effects of Isovitexin on femoral head necrosis, results showed that Isovitexin significantly improved femoral head necrosis by upregulating SIRT3 expression and modulating mitophagy-mediated ferroptosis.
Bone Res. 2025 Jan 26;13(1):18
Mice
S. aureus pneumonia model
Intraperitoneal injection
100 mg/kg
Every 12 hours
To evaluate the protective effect of isovitexin against S. aureus pneumonia. Results showed that isovitexin significantly improved the survival rate of infected mice, reduced the bacterial load in lung tissues, and alleviated pathological injury and inflammation in lung tissues.
J Microbiol Biotechnol. 2022 Oct 28;32(10):1284-1291
Sprague-Dawley rats
Acute gouty arthritis model
Intraperitoneal injection
100 mg/kg
Once daily for 1 week
Isovitexin significantly reduced the ankle joint swelling index, alleviated inflammatory cell infiltration, ameliorated synovial cell proliferation, decreased levels of TNF-α, IL-1β, and IL-6, and remarkably decreased the expression of TLR4, MyD88, and p-NF-κB-p65
Pharm Biol. 2021 Dec;59(1):1326-1333
BALB/c mice
LPS-induced acute lung injury model
Intraperitoneal injection
50 or 100 mg/kg
Single dose, lasting 12 hours
Isovitexin pretreatment attenuated histopathological changes, infiltration of polymorphonuclear granulocytes and endothelial activation, decreased the expression of ICAM-1 and VCAM-1, reduced the levels of MPO and MDA, and increased the content of GSH and SOD in ALI.
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