There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Usp14, a proteasome-associated deubiquitinating enzyme, can impede the degradation of ubiquitin-protein conjugates, both in vivo and in vitro. IU1 inhibits the catalytic activity of proteasome-associated Usp14 in vitro, with an IC50 of 4-5 μM. IU1 specifically binds to the activated form of Usp14 and may inhibit it by preventing its docking on the proteasome. However, direct tests of this scenario yielded negative results. Usp14 inhibition is rapidly established upon IU1 addition and rapidly reversed upon its removal. IU1 also promotes the degradation of Sic1, a CDK inhibitor from S. cerevisiae [1].
体外研究
Usp14, a proteasome-associated deubiquitinating enzyme, can impede the degradation of ubiquitin-protein conjugates, both in vivo and in vitro. IU1 inhibits the catalytic activity of proteasome-associated Usp14 in vitro, with an IC50 of 4-5 μM. IU1 specifically binds to the activated form of Usp14 and may inhibit it by preventing its docking on the proteasome. However, direct tests of this scenario yielded negative results. Usp14 inhibition is rapidly established upon IU1 addition and rapidly reversed upon its removal. IU1 also promotes the degradation of Sic1, a CDK inhibitor from S. cerevisiae [1].
IU1 细胞实验
Cell Line
Concentration
Treated Time
Description
References
USP14CAT
5 mM
1 hour
To evaluate the inhibitory effect of IU1 on USP14, results showed that IU1 inhibits USP14 activity by preventing substrate binding.
Cell Res. 2018 Dec;28(12):1186-1194.
CAD5 cells
50 µM
16 hours
To test whether IU1 could facilitate the clearance of polyubiquitinated substrates, results showed that IU1 treatment reduced the load of polyubiquitinated conjugates and PrPSc.
Acta Neuropathol. 2016 Mar;131(3):411-25.
GSC83 cells
25 µM
2 days
To evaluate the effect of IU1 on GSC83 cells, results showed that IU1 significantly downregulated ALDH1A3 expression and upregulated Tuj1 expression, leading to a reduction in GSC properties
Theranostics. 2025 Jan 13;15(6):2293-2314.
GSC83 cells
20 µM
24 hours
To evaluate the effect of IU1 on ALKBH5 protein levels, results showed that IU1 significantly reduced ALKBH5 protein levels
Theranostics. 2025 Jan 13;15(6):2293-2314.
HeLa cells
0.1, 0.2, 0.5, 1, 2, 5, 10, 20, 50, 100 µM
24 hours
IU1 significantly inhibited the proliferation of HeLa cells in a dose-dependent manner.
Int J Biol Sci. 2020 Sep 16;16(15):2951-2963.
SiHa cells
0.1, 0.5, 2, 5, 10, 20, 50, 100 µM
24 hours
IU1 significantly inhibited the proliferation of SiHa cells in a dose-dependent manner.
Int J Biol Sci. 2020 Sep 16;16(15):2951-2963.
KGN cells
50 µM
24 hours
Reduced ETO-induced DNA damage, promoted DDR function, and inhibited cell senescence
J Transl Med. 2024 Sep 11;22(1):834.
HCCLM3 cells
50 µM
24 or 48 hours
To assess the effect of IU1 on cell proliferation, results showed that 50 μM IU1 significantly reduced cell viability
Cell Death Dis. 2021 Aug 21;12(9):803.
MDA-MB453
75 µM
48 hours
To evaluate the effect of IU1 combined with enzalutamide on breast cancer cell proliferation and apoptosis, results showed that the combination significantly enhanced cell growth inhibition and apoptosis induction.
J Exp Clin Cancer Res. 2019 May 24;38(1):220.
MCF-7
75 µM
48 hours
To evaluate the effect of IU1 combined with enzalutamide on breast cancer cell proliferation and apoptosis, results showed that the combination significantly enhanced cell growth inhibition and apoptosis induction.
J Exp Clin Cancer Res. 2019 May 24;38(1):220.
Huh-7 cells
50 and 100 µM
To assess the effect of IU1 on cell migration and invasion, results showed that 50 and 100 μM IU1 significantly inhibited cell migration and invasion
Cell Death Dis. 2021 Aug 21;12(9):803.
IU1 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Athymic Nude mice
Brain tumor xenograft model
Intraperitoneal injection
25 μM
Once daily, continuous treatment
To evaluate the effect of IU1 on brain tumor xenograft models, results showed that IU1 significantly enhanced the cytotoxic effects of radiotherapy, significantly increasing survival rates
Theranostics. 2025 Jan 13;15(6):2293-2314.
BALB/c Nude mice
Subcutaneous xenograft and liver metastasis models
Intragastric administration
40 mg/kg
Every three days until the end of the experiment
IU1 administration inhibited tumour growth, reduced the expression of TAZ and Ki-67, decreased liver micrometastases, and improved survival rate.
Cell Death Differ. 2023 Jan;30(1):1-15.
Nude mice
HCCLM3 cell xenograft model
Oral
40 mg/kg
Every two days for 14 days
To assess the effect of IU1 on tumor growth, results showed that IU1 significantly inhibited tumor growth
Cell Death Dis. 2021 Aug 21;12(9):803.
Mice
D-galactose-induced POI model
Intraperitoneal injection
40 mg/kg
Once daily for 6 weeks
Improved D-gal-induced ovarian function decline, reduced DNA damage and cell senescence
J Transl Med. 2024 Sep 11;22(1):834.
BALB/c Nude mice
MCF-7 breast cancer xenograft model
Oral
40 mg/kg/d
Once daily for 17 days
To evaluate the effect of IU1 combined with enzalutamide on tumor growth in a breast cancer xenograft model, results showed that the combination significantly inhibited tumor growth.
J Exp Clin Cancer Res. 2019 May 24;38(1):220.
ICR mice
Ischemia-reperfusion injury model
Intraperitoneal injection
400 μg/kg
3 consecutive days
IU1 significantly downregulated the expression of USP14 in neurons and reduced neuronal apoptosis
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