Diphenhydramine (DPH) Hydrochloride is most commonly used via oral, topical, intramuscular (IM) and intravenous (IV) routes for the palliation of pruritus, treatment of extrapyramidal symptoms, management of parkinsonism and for allergic reactions. Subcutaneous diphenhydramine injection is a safe alternative to oral and other parenteral routes, and may be particularly valuable in terminally ill patients, who are often unable to swallow and are without IV access[3]. IV administration of diphenhydramine prior to MCT (mast cell tumor) excision had no clear clinical cardiorespiratory benefits over placebo in isoflurane-anesthetized dogs[4]. Intravenous diphenhydramine hydrochloride given before initiation of midazolam and pethidine offers a significant Improvement of quality of moderate sedation during colonoscopy without increasing the number of sedation related complications[5].
Diphenhydramine HCl/盐酸苯海拉明 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HEK293 cells expressing hTRPA1
1 mM
To test the effect of DPH on AITC-activated hTRPA1 currents. Results showed that 1 mM DPH completely suppressed outward currents while enhancing inward currents.
Cell Calcium. 2020 Sep;90:102245
HEK293 cells
100 μM and 1 mM
To test the effect of DPH on TRPA1 channels expressed in HEK293 cells. Results showed that 100 μM DPH had no effect on basal currents, while 1 mM DPH partially suppressed basal currents.
Cell Calcium. 2020 Sep;90:102245
Chinese hamster ovary (CHO) cells
1 mM
30 minutes
To investigate the inhibitory effect of diphenhydramine on histamine-induced JNK phosphorylation, results showed that diphenhydramine completely inhibited histamine-induced JNK phosphorylation.
Int J Mol Sci. 2024 Mar 17;25(6):3395
Diphenhydramine HCl/盐酸苯海拉明 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Female C57BL/6 mice
Experimental autoimmune encephalomyelitis (EAE) model
Intraperitoneal injection
10 mg/kg
Daily for 15 days
To investigate the effect of Diphenhydramine hydrochloride on EAE development, results showed that the drug significantly delayed the onset of EAE and reduced behavioral scores, while also reducing inflammatory cell infiltration and white matter demyelination in the spinal cord.
J Neurosci. 2016 Aug 31;36(35):9253-66
Mice
Wild-type and knockout mice
Carotid artery perfusion
1.4 μmol/L
60 seconds
To evaluate the transport mechanism of Diphenhydramine at the blood-retinal barrier, finding that its transport is mainly mediated by a proton antiporter with a lower passive diffusion rate.
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