c-Jun N-terminal kinase (JNK) is a member of the mitogen-activated protein kinases (MAPK) family. Anisomycin is an activator of both JNK and p38[3]. The incubation of HL-1 myocytes with 50ng/mL anisomycin for 24 h markedly increased the protein level of CaMKIIδ by ∼60% relative to the control group. The c-jun phosphorylation in total cell lysates and nuclear extracts was also increased by the treatment of 50ng/mL HL-1 myocytes with anisomycin for 24 h[4]. The intraperitoneal injection of wild-type, TNFR p55/p75-/-, and Nox2-/- mice with 0.1mg/kg anisomycin resulted in the activation of p38, an increase in the production of TNF-α and Nox2, and the phosphorylation of Nox2 compared with the control group[5].
Anisomycin/茴香霉素 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HeLa cells
50μM
20 minutes
To investigate the role of p38 in EphA2 Ser-897 phosphorylation, results showed anisomycin induced EphA2 phosphorylation via the p38 pathway.
J Biol Chem. 2023 May;299(5):104699
AML12 cells
10 μM
2 h
Activated JNK signaling pathway, increased TG accumulation, and partially deprived the therapeutic role of Tamoxifen
Signal Transduct Target Ther. 2023 Mar 3;8(1):94.
Cell monolayers derived from pig jejunum organoids
1 µM
24 h
Anisomycin disrupted the epithelial barrier integrity but failed to induce a significant increase in paracellular permeability. Butyrate (BUT) prevented the disruption of epithelial barrier integrity induced by ANI.
Gut Microbes. 2024 Jan-Dec;16(1):2430424.
SK-MES-1
0.5 µM
24 h
Anisomycin pre-treatment activated p38 and HSP27 and induced apoptosis.
Int J Biol Sci. 2020 Sep 2;16(15):2883-2894.
NCI-H520
0.5 µM
24 h
Anisomycin pre-treatment activated p38 and HSP27 and induced apoptosis.
Int J Biol Sci. 2020 Sep 2;16(15):2883-2894.
murine neonatal cardiomyocytes
25 ng/mL
1 hour
Pretreatment with anisomycin partially abolished the protective effects of NG-R1 against H/R-induced cardiomyocyte apoptosis.
Acta Pharmacol Sin. 2023 Jul;44(7):1366-1379.
Anisomycin/茴香霉素 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
MCD diet-induced NASH model
Intraperitoneal injection
50 mg/kg
5 consecutive days
Activated JNK signaling pathway and partially abolished the therapeutic effect of Tamoxifen on hepatic steatosis
Signal Transduct Target Ther. 2023 Mar 3;8(1):94.
Rats
Fear memory model
Bilateral infusion into the basolateral amygdala
125 mg/ml
Single administration
To evaluate the effect of Anisomycin on fear memory reconsolidation blockade, results showed that Anisomycin effectively blocked memory reconsolidation in rats trained with 1P, but had no effect in rats trained with 10P.
Elife. 2020 May 18;9:e57010
Mice
Depressive-like behavior model
Intraperitoneal injection
100 mg/kg
Single injection, behavioral tests performed 3 hours later
To evaluate the effect of Anisomycin on depressive-like behavior in mice, results showed that Anisomycin increased immobility time in TST and latency to feed in NSF.
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