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Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Protein kinase C (PKC) is a key molecule involved in cell growth, apoptosis, and differentiation. Phorbol 12-Myristate 13-Acetate (PMA) is an activator of certain types of PKC, including group A and group B isoforms[3]. Stimulation with 1 μM PMA for 3 min resulted in promoted nucleotide exchange activity in 293T cells[4]. In differentiated Caco-2 cells, pretreatment with 100 nM PMA for 5 min followed by 2h incubation with 10 nM 1,25(OH)2D3 resulted in a 2.6-fold increase in hCYP24A1 mRNA level compared to cells treated by 1,25(OH)2D3 alone. Incubation with 100 nM PMA for 30 min resulted in the activation of ERK1/2 and p38 kinase in Caco-2 cells, whereas pretreatment with the MEK inhibitor U0126 at 10, 20 or 100 μM completely inhibited ERK1/2 activation by PMA and partially suppressed PMA-induced p38 kinase activation. Cells pre-treated with 10 μM U0126 or 8 μM p38 kinase inhibitor SB202190 showed reduced enhancing effect of PMA on 1,25(OH)2D3-induced hCYP24A1 promoter activity[5]. In both W/Wv genetically mast cell-deficient mice and congenic normal mice, repeated administration of PMA (5 μg, 3 times per week for 6 weeks) to the ear skin remarkably increased the number of dermal mast cells[6].
Phorbol 12-myristate 13-acetate/佛波酯 细胞实验
Cell Line
Concentration
Treated Time
Description
References
NK cells
50 ng/ml
4 h
To evaluate NK cell activation by measuring granzyme B production.
Front Immunol. 2019 Jun 27;10:1467.
THP-1 cells
100 ng/mL
24 hours
Induced THP-1 cells to differentiate into macrophages for subsequent experiments
Acta Pharm Sin B. 2021 Nov;11(11):3493-3507.
THP-1 cells
100 ng/ml PMA
48 hours
Differentiate THP-1 cells into macrophages
Nat Microbiol. 2020 Sep;5(9):1088-1095
U937 cells
1 ng/mL
48 hours
To investigate the effect of monocyte differentiation into macrophages on KYN pathway activation. Results showed significant induction of IDO1 mRNA expression, suggesting that differentiation of monocytes into macrophages may promote KYN pathway activation.
Respir Res. 2024 Jan 14;25(1):31
THP1 cells
10 ng/mL
48 hours
To investigate the effect of monocyte differentiation into macrophages on KYN pathway activation. Results showed significant induction of IDO1 mRNA expression, suggesting that differentiation of monocytes into macrophages may promote KYN pathway activation.
Respir Res. 2024 Jan 14;25(1):31
THP-1 cells
100 ng/mL
48 hours
THP-1 cells were stimulated with PMA to differentiate into macrophages for the preparation of conditioned media.
Neoplasia. 2021 Jun;23(6):561-573
THP-1 cells
160 nM PMA and 100 ng/ml LPS
48 hours
Assess the anti-inflammatory capacity of the coating, results showed PEI/TIR/GS/OxHA significantly inhibited monocyte activation and intracellular ROS generation.
Bioact Mater. 2025 Feb 26;48:443-457
THP-1 cells
100 ng/mL PMA
48 hours
Differentiate THP-1 cells into macrophages for subsequent experiments.
J Exp Clin Cancer Res. 2024 Dec 26;43(1):332
Raw264.7 cells
200 nM
72 hours
To study the effect of VUF11207 on macrophage-like cells, results showed that VUF11207 reversed GAM-induced immunosuppression.
Cell Death Dis. 2024 Jun 19;15(6):434
THP-1 cells
200 nM
72 hours
To study the effect of VUF11207 on macrophage-like cells, results showed that VUF11207 reversed GAM-induced immunosuppression.
Cell Death Dis. 2024 Jun 19;15(6):434
UACC903 cells
10 nM
6 hours
TPA increased the expression and kinase activity of CK1ε and CK1δ.
Proc Natl Acad Sci U S A. 2018 Aug 7;115(32):E7522-E7531.
Proc Natl Acad Sci U S A. 2018 Aug 7;115(32):E7522-E7531.
MEG-01 cells
20 nM PMA
2 days
To study the role of DUSP4 in MEG-01 cell differentiation, it was found that DUSP4 mRNA and protein levels positively correlated with megakaryocyte differentiation.
Cell Rep. 2021 Jul 27;36(4):109421.
THP-1 cells
100 nM
48-72 hours
To induce THP-1 cell differentiation into M0 macrophages and further into TAMs to mimic the HCC tumor microenvironment.
J Immunother Cancer. 2022 Dec;10(12):e005655.
U937 cells
10 ng/mL
Induction of U937 cells into tumor-associated macrophages (TAMs)
Theranostics. 2018 Apr 30;8(11):3074-3086.
T-cells
5 ng/mL
16 h
Activate T-cells for studying their interaction with dendritic cells
Front Immunol. 2019 Mar 12;10:448.
CD4+Foxp3+Treg cells
50 ng/mL
4 h
stimulation for intracellular cytokine staining
Immunity. 2012 Sep 21;37(3):501-10.
B cells
50 ng/ml
4 h
To detect IL-6 and IL-10 expression in B cells, results showed that JDM patient B cells produced less IL-10 after TLR7 activation, while IL-6 expression was similar to healthy controls.
Front Immunol. 2018 Jun 22;9:1372.
Mouse skin cells
50 ng/mL
48 h
To evaluate the effect of PMA on IL-1β production in mouse skin cells. Results showed that PMA treatment significantly increased IL-1β production.
PLoS Pathog. 2017 Feb 13;13(2):e1006196.
3A9 T cells
100 nM
6 h
Used for T cell polarization experiments to observe T cell differentiation and cytokine production
J Exp Med. 2003 Apr 7;197(7):899-906.
NK cells
50 ng/ml
2 h
To evaluate NK cell activation by measuring IFN-γ and granzyme B production.
Front Immunol. 2019 Jun 27;10:1467.
Phorbol 12-myristate 13-acetate/佛波酯 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Irritant contact dermatitis
Topical administration
0.08 μM
Single dose, imaging after 6 hours
To validate the specificity of PMA in inducing inflammation, results showed increased fluorescence signal in the PMA-treated hindpaw of wildtype mice, while no fluorescence signal was detected in MPO-KO mice.
Germany
... 展开 >> Technical University of Dortmund, Leibniz Research Centre for Working Environment and Human Factors
Recruiting
Dortmund, Germany, 44139
Contact: Carsten Watzl, PhD +492311084233 watzl@ifado.de
University Medical Center Leipzig, Department of Medical Psychology and Medical Sociology, Section Psychosocial Oncology
Active, not recruiting
Leipzig, Germany, 04103 收起 <<
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