Facilitated ABC294640-induced cytotoxicity against HT-29 cells
J Exp Clin Cancer Res. 2015 Sep 4;34(1):94.
NCI/ADR-RES cells
25 μM
48 hr
C6-ceramide substantially increased the levels of phosphorylated p53 and enhanced the expression of p53-responsive genes such as p21Waf1/Cip1, Bax, and Puma.
Cancer Res. 2011 Mar 15;71(6):2276-85
p56lck-reconstituted JCaM1.6 cells
10 μM
15-25 min
Reconstitution of p56lck partially restored the inhibitory effect of C6-ceramide on n-K1 channels.
Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7661-6
p56lck-deficient JCaM1.6 cells
10 μM
20 min
In p56lck-deficient cells, the inhibitory effect of C6-ceramide on n-K1 channels was significantly reduced.
Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7661-6
Jurkat T lymphocytes
10 μM
15-25 min
C6-ceramide significantly inhibited n-K1 channel currents, with a 41±5% decrease at 15 min and 67±6% decrease at 25 min.
Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7661-6
Human adipose derived stem cells (hADSC)
20 µM
48 h
C6-ceramide increased cellular and exosomal MALAT1 levels and promoted migration and mitochondrial function in human dermal fibroblasts.
Cell Commun Signal. 2023 Aug 24;21(1):221
Bone marrow-derived macrophages (BMMs)
25 µM
24 h
LipC6 significantly inhibited ROS production in both GMCSF-induced M1 BMMs and MCSF-induced M2 BMMs, promoted M1 cytokine production, and suppressed M2 cytokine production.
Gastroenterology. 2018 Mar;154(4):1024-1036. e9
HEK293 cells
40 μM
24 h
To evaluate the effect of CNL on non-transformed cells. CNL treatment did not affect the viability of HEK293 cells or STAT3 phosphorylation.
Signal Transduct Target Ther. 2017 Oct 27;2:17051
Mec-2 cells
40 μM
24 h
To evaluate the effect of CNL on STAT3 phosphorylation. A reduction in STAT3 phosphorylation at Y705 and S727 residues was observed.
Signal Transduct Target Ther. 2017 Oct 27;2:17051
JVM-3 cells
40 μM
24 h
To evaluate the effect of CNL on STAT3 phosphorylation. A reduction in STAT3 phosphorylation at both Y705 and S727 residues was observed in six out of seven patient samples, while total STAT3 levels remained unchanged.
Signal Transduct Target Ther. 2017 Oct 27;2:17051
C6 Ceramide 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Chick embryos
CAM assay
Topical application
50 μM
24 hours
Reduced new vessel formation
Int J Biol Sci. 2011;7(5):629-44
C57BL/6 mice
Hepatocellular carcinoma model in a fibrotic/cirrhotic liver setting
Tail vein injection
35 mg/kg
Every other day for 2 weeks
LipC6 injection slowed tumor growth, reduced the number and immunosuppressive function of tumor-associated macrophages (TAMs), and promoted TAM differentiation into an M1 phenotype, thereby enhancing the antitumor activity of CD8+ T cells.
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