Blebbistatin, a selective non-muscle myosin II (NMII) inhibitor, enhances directional migration of corneal endothelial cells (CECs), accelerates wound healing, and preserves cell junctional integrity and barrier function. It impedes cell migration [1][2].
体内研究
Blebbistatin (0.05 mL, 20 μM; intracameral injection; daily; for 6 days; New Zealand white rabbits) facilitates wound healing in a rabbit corneal endothelial scraping model [1].
体外研究
Investigating the therapeutic potential of targeting NMII to boost CEC migration, blebbistatin promotes migration and directional persistence in CECs, thus expediting wound healing in vitro by reducing actin retrograde flow and increasing lamellipodial protrusion persistence [1].
Blebbistatin 细胞实验
Cell Line
Concentration
Treated Time
Description
References
human iPSC-derived motor neurons
25 μM
24 to 72 h
promotes neurite outgrowth and branching
Nat Commun. 2025 Mar 26;16(1):2975
NIH 3T3 cells
1 μM and 5 μM
7 h
Evaluating the effect of Blebbistatin on cell migration, results showed cell migration speed decreased from 15.7 μm/h (control) to 8.8 μm/h (1 μM) and 7.5 μm/h (5 μM).
Biosens Bioelectron. 2021 Dec 1;193:113533
U87 glioblastoma cells
100 µM
24 h
To assess the effect of myosin II inhibition on protrusion formation in U87 cells. Results showed that blebbistatin treatment increased protrusion length but reduced alignment of protrusions to fibers.
Biomaterials. 2019 Mar;197:101-118
Human foreskin fibroblast (HFF) cells
100 µM
24 h
To assess the effect of myosin II inhibition on protrusion formation in HFF cells. Results showed that blebbistatin treatment did not affect protrusion length but reduced alignment of protrusions to fibers.
Biomaterials. 2019 Mar;197:101-118
Dictyostelium myosin II
100 μM
Inhibits the ATPase activity of Dictyostelium myosin II with an IC50 of 7 μM and 95% inhibition at 100 μM
Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1472-7.
mouse hepatic stellate cells
12.5, 25, 50 μM
24 h
To study the effects of blebbistatin on cell migration. Results showed that blebbistatin promoted wound-induced cell migration.
Br J Pharmacol. 2010 Jan 1;159(2):304-15
mouse hepatic stellate cells
50 μM
2 h
To study the effects of blebbistatin on intracellular calcium release. Results showed that blebbistatin blocked endothelin-1-induced intracellular calcium release.
Br J Pharmacol. 2010 Jan 1;159(2):304-15
Blebbistatin 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6J mice
FeCl3-induced carotid artery thrombosis model
Intraperitoneal injection
1 mg/kg
Single dose, observed for 60 minutes
To investigate the effect and potential mechanism of blebbistatin on FeCl3-induced carotid artery thrombosis. Results showed that blebbistatin significantly reduced carotid artery thrombosis, inhibited NMMHC IIA expression, and impeded TF expression via the GSK3β-NF-κB signalling pathway.
Int J Biol Sci. 2017 May 15;13(5):630-639
Zebrafish
Transgenic Tg(fli:EGFP) zebrafish larvae
Water solution
100 µM
Single dose, 24 hours duration
To assess the effect of myosin II inhibition on U87 cell alignment to blood vessels in zebrafish brain. Results showed that blebbistatin treatment reduced alignment of U87 cells to blood vessels.
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