Golgicide A serves as a powerful, highly selective, and reversible antagonist of GBF1, which is an ADP-ribosylation factor guanine nucleotide exchange factor (ArfGEF) associated with the cis-Golgi. It works as a strong and highly efficient inhibitor of shiga toxin's action. It effectively blocks the impact of shiga toxin on protein synthesis, exhibiting an IC50 value of 3.3 μM[1]. Golgicide A significantly inhibits the replication of coxsackievirus B3 (CVB3) as well as other types of human enteroviruses[2].
Golgicide A 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HeLa-ATCC cells
20 μM
5 h
Enhanced HAdV-C2-dE3B_GFP infection
J Virol. 2014 Nov;88(22):13086-98.
A549 cells
20 μM
5 h
Enhanced HAdV-C5-dE1_GFP infection
J Virol. 2014 Nov;88(22):13086-98.
primary astrocytes
3.5 μM
8 h
GCA reduced basal secreted Aβ levels
Cells. 2022 Aug 19;11(16):2585.
293sw cells
3.5 μM
8 h
GCA reduced basal secreted Aβ levels
Cells. 2022 Aug 19;11(16):2585.
WI38 fibroblasts
20 μM
5 h
Not used
J Virol. 2014 Nov;88(22):13086-98.
M950822 and M980928 melanoma cells
20 μM
5 h
Enhanced HAdV-C5-dE1_GFP infection
J Virol. 2014 Nov;88(22):13086-98.
SF188 cells
10 μM
14 h
PTEN knockdown significantly increased cell sensitivity to secretion inhibitors BFA and GCA, leading to increased cell death.
Nat Commun. 2018 Jun 19;9(1):2392.
Vero cells
10 μM
1 hour
GCA treatment resulted in complete dispersal of the Golgi and TGN, similar to the effects of BFA.
Nat Chem Biol. 2009 Mar;5(3):157-65.
BEAS-2B bronchial epithelial cells
4 µM
24 h
Artificial induction of Golgi fragmentation, reducing bacterial adherence but increasing bacterial replication
Cell Commun Signal. 2023 Aug 17;21(1):208.
BEAS-2B bronchial epithelial cells
4 µM
4 h
Artificial induction of Golgi fragmentation, reducing bacterial adherence but increasing bacterial replication
Cell Commun Signal. 2023 Aug 17;21(1):208.
HEL cells
10 µM
48 h
Golgicide A blocked STING exocytosis from HSV-1-infected cells, indicating that STING trafficking through the Golgi is required.
mBio. 2024 Apr 10;15(4):e0037324.
BHK-21 cells
27 μM
24 h
Evaluate the effect of Golgicide A on DENV NS1 secretion, results showed NS1 secretion was reduced more than 70% in vertebrate cells
J Virol. 2019 Feb 5;93(4):e01985-18.
Aag2 cells
27 μM
24 h
Evaluate the effect of Golgicide A on DENV NS1 secretion, results showed no effect on NS1 secretion in mosquito cells
J Virol. 2019 Feb 5;93(4):e01985-18.
C6/36 cells
27 μM
24 h
Evaluate the effect of Golgicide A on DENV NS1 secretion, results showed no effect on NS1 secretion in mosquito cells
J Virol. 2019 Feb 5;93(4):e01985-18.
MA104 cells
10 μM
12 h
Evaluate the effect of GCA on rotavirus replication, showing a significant reduction in viral yield
J Virol. 2019 Sep 12;93(19):e01062-19.
Golgicide A 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
BALB/c nude mice
Subcutaneous xenograft model
Subcutaneous injection
20 mg/kg and 50 mg/kg
Once a week for 4 weeks
Evaluate the inhibitory effect of GCA on tumor growth
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