Acetyl shikonin, a natural product isolated and purified from the roots of Lithosperraum erythrorhizon Sieb. et Zucc., inhibits the generation of NADPH oxidase complex in the activation of respiratory burst of PMNs, but does not directly inhibit the activity of NADPH oxidase already generated, and is a non-selective cytochrome P450 inhibitor.
Acetylshikonin/乙酰紫草素 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HCT-15
HCT-15
24, 48, 72 h
Suppressed cell proliferation by inducing G1 phase cell cycle arrest and apoptosis
Br J Pharmacol. 2020 May;177(10):2303-2319.
HCT-116
HCT-116
24, 48, 72 h
Suppressed cell proliferation by inducing G2 phase cell cycle arrest and apoptosis
Br J Pharmacol. 2020 May;177(10):2303-2319.
SW 620
SW 620
24, 48, 72 h
Suppressed cell proliferation by inducing G3 phase cell cycle arrest and apoptosis
Br J Pharmacol. 2020 May;177(10):2303-2319.
DLD-1
DLD-1
24, 48, 72 h
Suppressed cell proliferation by inducing G4 phase cell cycle arrest and apoptosis
Br J Pharmacol. 2020 May;177(10):2303-2319.
Lung single cells
Lung single cells
12 h
Acetylshikonin down-regulated the production of IL-6 in a dose-dependent manner, with significant reduction at 2.5 μM concentration.
Signal Transduct Target Ther. 2022 Oct 27;7(1):371.
BMDM cells
BMDM cells
12 h
Acetylshikonin down-regulated the production of IL-6 in a dose-dependent manner, with significant reduction at 2.5 μM concentration.
Signal Transduct Target Ther. 2022 Oct 27;7(1):371.
RAW264.7 cells
RAW264.7 cells
12 h
Acetylshikonin down-regulated the production of IL-6 in a dose-dependent manner, with significant reduction at 2.5 μM concentration.
Signal Transduct Target Ther. 2022 Oct 27;7(1):371.
MEF cells and MEF Nur77−/− cells
MEF cells and MEF Nur77−/− cells
24 h
SK07 induced apoptosis in MEF cells, but its effect was significantly reduced in Nur77 knockout cells.
Cancer Res. 2008 Nov 1;68(21):8871-80.
HeLa cervical cancer cells
HeLa cervical cancer cells
12 or 24 h
Acetylshikonin and its analog SK07 significantly increased Nur77 protein levels in HeLa cells.
Cancer Res. 2008 Nov 1;68(21):8871-80.
NIH-H460 lung cancer cells
NIH-H460 lung cancer cells
12 or 24 h
Acetylshikonin and its analog SK07 significantly increased Nur77 protein levels, while SK06 had a weaker effect.
Cancer Res. 2008 Nov 1;68(21):8871-80.
mouse Lewis lung carcinoma cell line LLC
mouse Lewis lung carcinoma cell line LLC
48 h
Acetylshikonin inhibited the growth of LLC cells in a dose-dependent manner with IC50 of 2.72 ± 0.38 μg/ml.
Chin Med. 2009 Jul 11;4:14.
human breast adenocarcinoma cell line MCF-7
human breast adenocarcinoma cell line MCF-7
48 h
Acetylshikonin inhibited the growth of MCF-7 cells in a dose-dependent manner with IC50 of 3.04 ± 0.44 μg/ml.
Chin Med. 2009 Jul 11;4:14.
human hepatocellular carcinoma cell line Bel-7402
human hepatocellular carcinoma cell line Bel-7402
48 h
Acetylshikonin inhibited the growth of Bel-7402 cells in a dose-dependent manner with IC50 of 6.82 ± 1.5 μg/ml.
Chin Med. 2009 Jul 11;4:14.
human lung adenocarcinoma cell line A549
human lung adenocarcinoma cell line A549
48 h
Acetylshikonin inhibited the growth of A549 cells in a dose-dependent manner with IC50 of 5.6 ± 0.86 μg/ml.
Chin Med. 2009 Jul 11;4:14.
Rat neutrophils
Rat neutrophils
3 min
Acetylshikonin inhibited fMLP/CB-induced superoxide anion generation and oxygen consumption with IC50 values of 0.48 μM and 0.39 μM, respectively.
Br J Pharmacol. 1997 Jun;121(3):409-16.
Rat neutrophils
Rat neutrophils
3 min
Acetylshikonin inhibited PMA-induced superoxide anion generation and oxygen consumption with IC50 values of 0.48 μM and 0.39 μM, respectively.
Br J Pharmacol. 1997 Jun;121(3):409-16.
U87 human GBM cells
U87 human GBM cells
72 h
To evaluate the cytotoxic effects of Acetylshikonin on U87 cells, results showed that all concentrations of ASH induced significant cell death.
Biology (Basel). 2023 Feb 14;12(2):302.
U251 human GBM cells
U251 human GBM cells
72 h
To evaluate the cytotoxic effects of Acetylshikonin on U251 cells, results showed that all concentrations of ASH induced significant cell death.
Biology (Basel). 2023 Feb 14;12(2):302.
THP-1 cells
THP-1 cells
24 h
Acetylshikonin significantly inhibited the proliferation of THP-1 cells.
J Cell Mol Med. 2022 Mar;26(5):1606-1620.
K562 cells
K562 cells
24 h
Acetylshikonin significantly inhibited the proliferation of K562 cells.
J Cell Mol Med. 2022 Mar;26(5):1606-1620.
HL-60 cells
HL-60 cells
24–48 h
Acetylshikonin significantly inhibited the proliferation and viability of HL-60 cells and induced apoptosis through the mitochondrial pathway, while promoting S-phase cell cycle arrest.
J Cell Mol Med. 2022 Mar;26(5):1606-1620.
Acetylshikonin/乙酰紫草素 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
BALB/c nude mice
Ba/F3 EGFR ASV and L861Q allografts model
Oral
20 and 40 mg/kg
Once daily for 13 days
Evaluate the inhibitory effect of Almonertinib on tumor growth with EGFR ASV and L861Q mutations
Br J Pharmacol. 2020 May;177(10):2303-2319.
BALB/c mice
Acute lung injury model
Intragastric administration
300 mg/kg
Three consecutive days
Acetylshikonin significantly decreased the concentrations of IL-6, TNF-α and IL-1α in BALF and suppressed symptoms of interstitial pneumonia in a concentration-dependent manner.
Signal Transduct Target Ther. 2022 Oct 27;7(1):371.
C57BL/6 mice
Lewis lung carcinoma model
Intraperitoneal injection
0.5, 1, 2 mg/kg
Once every two days, six times in total
Acetylshikonin (2 mg/kg) suppressed LLC tumor growth with an inhibition rate of 42.85%. Immunohistochemical staining showed increased expression of bax and caspase-3 and decreased expression of bcl-2 in acetylshikonin groups.
搜索
