Y-27632 is a specific inhibitor of Rho-associated kinases[1]. It is usually used for ES cell maintance[2], cell reprogramming[3],improving cell suviving after thawing[4], as well as facilitates cell proliferation and migration[5].
作用机制
Y-27632 can inhibit ROCK1 and ROCK2 in an ATP-competition manner.[1]
Y-27632 细胞实验
Cell Line
Concentration
Treated Time
Description
References
ARPE19 cells
40µM
24 h
Y-27632 treatment significantly increased nanotube formation and mitochondrial transfer rate
Theranostics. 2024 Sep 9;14(15):5762-5777.
ARPE19 cells
10µM
24 h
Y-27632 treatment led to an increase in nanotube formation and enhanced mitochondrial transfer among ARPE19 cells
Theranostics. 2024 Sep 9;14(15):5762-5777.
Y-27632 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
New Zealand white rabbits
Glaucoma model
Topical instillation
2% w/v, 50 μL
Single dose, lasting for 10 days
To evaluate the therapeutic effect of HMCNs on glaucoma, results showed that HMCNs with a 20 nm shell thickness effectively reduced intraocular pressure and repaired damaged cornea and retina.
Theranostics. 2021 Mar 13;11(11):5447-5463
Mice
OVA sensitised and RSV infected mouse model
Oral gavage
30 mg/kg
Administered 2 hours before methacholine challenge
Y-27632 significantly reduced baseline lung resistance in OVA sensitised mice and suppressed methacholine and serotonin induced airway hyperresponsiveness in both OVA sensitised and OVA/RSV mice
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