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Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Nigericin sodium is an antibiotic from Streptomyces hygroscopicus that works by acting as an H+, K+, and Pb2+ ionophore, a NLRP3 activator. Nigericin (0.1 µM) inhibits proliferation, migration, invasion and clonogenicity of H460 lung cancer cells[3]. A 48-hr exposure of human erythrocytes to nigericin significantly increased the percentage of annexin-V-binding cells (0.1-10 nM) and Fluo3 fluorescence (0.1-10 nM), significantly decreased forward scatter (0.1-1 nM) and cytosolic pH (0.1-1 nM). Nigericin (1 nM) slightly, but significantly, increased ROS, but did not significantly modify ceramide abundance[4]. Nigericin could selectively target CSCs (Cancer stem cells) and sensitize CSCs in NPC (Nasopharyngeal carcinoma) to the widely used clinical drug cisplatin both in vitro and in vivo[5]. Nigericin exhibits great toxicity for the HT29 and SW116 cell line with IC50 of 12.92±0.25 μM and 15.86±0.18 μM. Nigericin also shows a decreased ability to form colonies under anchorage-independent conditions. HT29 cells treated with nigericin induced an increase in E-cadherin expression and a decrease in the vimentin expression relative to vehicle controls[6].
Nigericin sodium salt/尼日利亚菌素钠盐 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Bone marrow-derived macrophages (BMDMs)
1 µM
1 hour
To investigate whether DSS potentiates NLRP3 inflammasome activation. Results showed that DSS significantly enhanced nigericin-induced NLRP3 inflammasome activation.
Cell Mol Immunol. 2022 Aug;19(8):925-943.
BACH1-deficient macrophages
20 μM
15 min
To observe the activation of the NLRP3 inflammasome, results showed that in BACH1-deficient macrophages, nigericin treatment significantly increased the secretion of IL-1β and IL-18 after LPS stimulation.
Redox Biol. 2022 May;51:102265.
N/TERT cells
5 µg/mL
3 h
Nigericin inhibits protein synthesis through potassium efflux, leading to ribosome stalling and activation of ZAKα-dependent ribotoxic stress response (RSR).
Proc Natl Acad Sci U S A. 2024 Jan 9;121(2):e2309579121.
primary keratinocytes
5 µg/mL
24 h
Nigericin induces IL-1β secretion, and this process depends on NLRP1 and potassium efflux.
Proc Natl Acad Sci U S A. 2024 Jan 9;121(2):e2309579121.
primary human skin, nasal, and corneal epithelial cells
6.7 µM
3 h
Validate that nigericin activates the NLRP1 inflammasome, leading to pyroptosis, characterized by membrane swelling, GSDMD cleavage, ASC speck formation, rapid PI uptake, and IL-1 secretion.
Proc Natl Acad Sci U S A. 2024 Jan 9;121(2):e2309579121.
BV2 cells
10 µM
45 min
Nigericin was used to activate the NLRP3 inflammasome, promoting pyroptosis and the secretion of inflammatory cytokines in BV2 cells. The results showed that after Nigericin treatment, the protein expression of NLRP3, Caspase-1 P20, and GSDMD-N in BV2 cells significantly increased, and the mRNA levels of inflammatory factors IL-1β, TNF-α, and IL-6 also significantly increased.
J Neuroinflammation. 2022 Sep 28;19(1):237.
Primary mouse microglia
10 µM
45 min
Nigericin was used to activate the NLRP3 inflammasome, promoting microglial pyroptosis and the secretion of inflammatory cytokines. The results showed that after Nigericin treatment, the protein expression of NLRP3, Caspase-1 P20, and GSDMD-N in microglia significantly increased, and the secretion of inflammatory factors IL-1β, IL-18, TNF-α, and IL-6 also significantly increased.
J Neuroinflammation. 2022 Sep 28;19(1):237.
THP-1 macrophages
5 μg/mL
60 min
Induced pyroptosis, TFG-deficient THP-1 cells exhibited higher mitochondrial ROS production and pyroptotic cell death
Cell Death Dis. 2022 Jan 28;13(1):93.
HL-1 cardiomyocytes
5 μM
1, 3, 6, 9, 12 h
To investigate the subcellular localization changes of GSDMD-N under Nigericin-induced inflammatory stress, results showed a time-dependent translocation of GSDMD-N from mitochondria to cytoplasmic membrane.
Clin Transl Med. 2022 Aug;12(8):e1002.
Mouse peritoneal macrophages (MPMs)
1 μM
12 h
Activate NLRP3 inflammasomes to simulate an inflammatory environment and study the anti-inflammatory effects of hucMSC-derived exosomes. Results showed that hucMSC-derived exosomes significantly reduced NLRP3 inflammasome activation and IL-1β release.
Stem Cell Res Ther. 2021 Jul 22;12(1):416.
THP-1 cells
1 μM
12 h
Activate NLRP3 inflammasomes to simulate an inflammatory environment and study the anti-inflammatory effects of hucMSC-derived exosomes. Results showed that hucMSC-derived exosomes significantly reduced NLRP3 inflammasome activation and IL-1β release.
Stem Cell Res Ther. 2021 Jul 22;12(1):416.
AML12 cells
20 mM
24 h
To study the induction of PANoptosis by Nigericin under lipid stress. Results showed that Nigericin in combination with TNF-α and LPS significantly aggravated cell injury, as evidenced by decreased cell viability and increased LDH release.
Acta Pharmacol Sin. 2023 May;44(5):1014-1028.
bone marrow-derived macrophages (BMDMs)
5 µM
1 hour
Inhibited NLRP3 inflammasome activation, reduced release of caspase-1p10 and mature IL-1β.
Acta Pharmacol Sin. 2023 Oct;44(10):2019-2036.
mouse macrophage cell line J774A.1
5 µM
1 hour
Inhibited NLRP3 inflammasome activation, reduced release of caspase-1p10 and mature IL-1β.
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