Indole-3-carbinol (I3C) inhibits NF-κB activity and also is an Aryl hydrocarbon receptor (AhR) agonist, and an inhibitor of WWP1 (WW domain-containing ubiquitin E3 ligase 1). I3C inhibits the proliferation of THP-1 cells in a dose- and time-dependent manner with minimal toxicity over normal monocytes. I3C could exert its antileukemic effects through AhR activation which is associated with programed cell death and G1 cell cycle arrest in a dose- and time-dependent manner[3]. Treatment with I3C led to repressed colonic inflammation and prevention of microbial dysbiosis caused by colitis, increasing a subset of gram-positive bacteria known to produce butyrate. I3C attenuates colitis primarily through induction of IL-22, which leads to modulation of gut microbiota that promote antiinflammatory butyrate[4]. I3C prevented EtOH (ethanol) -induced liver injury via attenuating the damaging effect of ethanol on the gut-liver-adipose tissue axis[5]. I3C may induce apoptosis in human osteosarcoma MG-63 and U2OS cells via the activation of apoptotic signaling pathways by FOXO3[6].
Indole-3-carbinol/3-吲哚甲醇 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Cutibacterium acnes
0.1 mM
6 days
significantly inhibited biofilm formation by C. acnes without affecting planktonic cell growth
Microbiol Spectr. 2022 Feb 23;10(1):e0205621.
Cutibacterium acnes
0.1 mM
6 days
Significantly inhibited biofilm formation by C. acnes without affecting planktonic cell growth
Microbiol Spectr. 2022 Feb 23;10(1):e0205621.
Jurkat cells
10, 50 µM
48 h
I3C pretreatment significantly increased PMA/ionomycin-induced IL-2, IL-8, and TNF-α production and enhanced inflammatory responses through activation of NF-κB and NFAT1 signaling pathways.
Int J Mol Sci. 2017 Jul 1;18(7):1409.
Indole-3-carbinol/3-吲哚甲醇 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Clostridium difficile infection model
Dietary supplementation
1000 ppm
Two weeks
To investigate the impact of I3C on CDI, results showed that I3C significantly improved survival, reduced weight loss, and disease scores in mice.
Ann Surg. 2017 Jun;265(6):1183-1191
Mice
Hi-Myc mouse model
Intraperitoneal injection
20 mg/kg
Three times per week for one month
Investigated the therapeutic effects of I3C on prostate tumors in Hi-Myc mice, showing that I3C inhibited tumor growth and AKT activation.
United States, Washington
... 展开 >>
Brabant Research, Incorporated
Not yet recruiting
Spokane Valley, Washington, United States, 99216
Contact: Richard E. Lasker, PhD 509-340-9902 rel@brabantresearch.com 收起 <<
United States, Washington
... 展开 >>
Brabant Research, Incorporated
Not yet recruiting
Spokane Valley, Washington, United States, 99216
Contact: Richard E. Lasker, PhD 509-340-9902 rel@brabantresearch.com 收起 <<
Unspecified Adult Solid Tumor,... 展开 >> Protocol Specific 收起 <<
Phase 1
Completed
-
United States, Kansas
... 展开 >>
Kansas Masonic Cancer Research Institute at the University of Kansas Medical Center
Kansas City, Kansas, United States, 66160-7357 收起 <<
United States, Oregon
... 展开 >>
Oregon Health & Science University
Recruiting
Portland, Oregon, United States, 97201
Contact: Matthew French 503-494-7004 frenchm@ohsu.edu
Contact: Carrie Farrar 503-494-4233 farrarc@ohsu.edu
Principal Investigator: David Lieberman, MD 收起 <<
United States, Minnesota
... 展开 >>
Masonic Cancer Center at University of Minnesota
Recruiting
Minneapolis, Minnesota, United States, 55455
Contact: Hanna Vanderloo, RN, MSN 收起 <<
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