There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
DCPIB is a selective, reversible and potent inhibitor of VRAC. DCPIB voltage-dependently activates the potassium channels TREK1 and TRAAK, and inhibits TRESK, TASK1, and TASK3 (IC50 values of 0.14, 0.95, and 50.72 μM, respectively). DCPIB is also a selective and swelling-induced blocker of chloride current with an IC50 value of 4.1 μM. DCPIB is an effective tool to study the structure-function of K2P channels. DCPIB at a concentration of 10 μM activates TREK1 and enhances TRAAK currents in COS-7 cells[1][2]. At the same concentration, DCPIB significantly and reversibly inhibited TRESK current in COS-7 cells with an IC50 of 0.14 μM[1]. While acting for 3 hours, DCPIB inhibited LPS-induced MAPK activation in BV2 cells[3].
DCPIB 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HEK-293 cells
HEK-293 cells
SN-401 inhibited ICl,SWELL currents with an IC50 of 3.9 µM
Nat Commun. 2022 Feb 10;13(1):784.
HK-2 cells
HK-2 cells
Inhibited TGFβ1-induced chloride currents and GSH conductance
Cell Death Dis. 2019 Dec 5;10(12):925.
cardiomyocytes
cardiomyocytes
alleviated tunicamycin-induced ER stress and apoptosis
Cell Death Dis. 2014 Nov 20;5(11):e1528.
human umbilical vein endothelial cells (HUVECs)
human umbilical vein endothelial cells (HUVECs)
SN-401 increased eNOS phosphorylation (Ser1177), and this effect was abrogated by SWELL1 knockdown in HUVECs
Nat Commun. 2022 Feb 10;13(1):784.
3T3-F442A adipocytes
3T3-F442A adipocytes
SN-401 increased SWELL1 protein expression 1.5-fold and enhanced insulin-stimulated pAKT2 and pAS160 signaling
Nat Commun. 2022 Feb 10;13(1):784.
3T3-F442A preadipocytes
3T3-F442A preadipocytes
SN-401 increased SWELL1 protein expression threefold and enhanced insulin-stimulated pAKT2 signaling
Nat Commun. 2022 Feb 10;13(1):784.
HL-1 cardiomyocytes
HL-1 cardiomyocytes
Inhibited ET-1-induced volume-sensitive Cl⁻ current (ICl,swell)
Cardiovasc Res. 2010 Oct 1;88(1):93-100.
rabbit ventricular myocytes
rabbit ventricular myocytes
Inhibited ET-1-induced volume-sensitive Cl⁻ current (ICl,swell)
Cardiovasc Res. 2010 Oct 1;88(1):93-100.
rabbit atrial myocytes
rabbit atrial myocytes
Inhibited ET-1-induced volume-sensitive Cl⁻ current (ICl,swell)
Cardiovasc Res. 2010 Oct 1;88(1):93-100.
rabbit ventricular myocytes
rabbit ventricular myocytes
DCPIB inhibited the volume-sensitive chloride current activated by C2-Cer and bacterial SMase
DCPIB inhibited NF-κB activation in a concentration-dependent manner
Free Radic Biol Med. 2016 Dec;101:413-423.
PC12 cells
PC12 cells
To evaluate the inhibitory effect of DCPIB on hypotonic-induced changes in intracellular chloride concentration, results showed DCPIB blocked hypotonic-induced decrease in [Cl–]i
Acta Pharmacol Sin. 2013 Jan;34(1):113-8.
PC12 cells
PC12 cells
To evaluate the inhibitory effect of DCPIB on OGD-induced cell death, results showed DCPIB significantly reduced OGD-induced cell death
Acta Pharmacol Sin. 2013 Jan;34(1):113-8.
primary cultured cortical neurons
primary cultured cortical neurons
To investigate the inhibitory effect of DCPIB on the swelling-induced chloride current (ICl,swell). Results showed that DCPIB completely inhibited the ICl,swell activated by a 30% hypotonic solution.
Acta Pharmacol Sin. 2018 May;39(5):858-865.
COS-7 cells
COS-7 cells
Test the modulatory effect of DCPIB on TWIK2 channel, results showed no significant inhibition on TWIK2 channel
Acta Pharmacol Sin. 2022 Apr;43(4):992-1000.
DCPIB 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Obese, HFD-fed T2D model and polygenic-T2D KKAy mouse model
Intraperitoneal injection
5 mg/kg
Once daily for 4–10 days
SN-401 increased SWELL1 expression in adipose tissue (twofold in HFD mice, threefold in KKAy mice), improved fasting blood glucose, glucose tolerance, and insulin tolerance, increased insulin sensitivity, and reduced hepatic steatosis and hepatocyte damage
Nat Commun. 2022 Feb 10;13(1):784.
CD1 mice
Neonatal hypoxic-ischemic injury model
Intraperitoneal injection
10 mg/kg
Single dose, 20 minutes before ischemia
To evaluate the neuroprotective effect of DCPIB on neonatal hypoxic-ischemic injury, results showed DCPIB significantly reduced brain infarct volume and improved neurobehavioral outcomes
Acta Pharmacol Sin. 2013 Jan;34(1):113-8.
CD1 mice
Neonatal mouse hypoxic-ischemic brain injury model
Intraperitoneal injection
10 mg/kg
Single dose, approximately 20 minutes before ischemia
To evaluate the protective effects of DCPIB on neonatal hypoxic-ischemic brain injury. Results showed that pretreatment with DCPIB significantly reduced brain damage (assessed by TTC staining, Nissl staining, and whole brain imaging) and improved sensorimotor and vestibular recovery outcomes in neurobehavioral tests (i.e., geotaxis reflex and cliff avoidance reflex).
搜索
HazMat Fee +
