Chrysin, found in passion flowers, honey, and propolis, acts as a potential therapeutic and preventive agent against various human cancers, including ovarian cancer. It inhibits ovarian cancer cell proliferation and induces cell death by increasing ROS production, cytoplasmic Ca2+ levels, and inducing loss of mitochondrial membrane potential. Chrysin activates both MAPK and PI3K/AKT pathways, suppresses tumor growth by regulating Wnt and NF-κB signaling, and stimulates phosphorylation of AKT and P70S6K proteins[1].
体外研究
Chrysin, found in passion flowers, honey, and propolis, acts as a potential therapeutic and preventive agent against various human cancers, including ovarian cancer. It inhibits ovarian cancer cell proliferation and induces cell death by increasing ROS production, cytoplasmic Ca2+ levels, and inducing loss of mitochondrial membrane potential. Chrysin activates both MAPK and PI3K/AKT pathways, suppresses tumor growth by regulating Wnt and NF-κB signaling, and stimulates phosphorylation of AKT and P70S6K proteins[1].
Chrysin/白杨素 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Mouse embryonic fibroblasts
25 µM
24 hours
Investigate the effect of xanthosine on cellular metabolome, results showed xanthosine downregulated metabolites involved in cholesterol and uric acid synthesis pathways
Int J Mol Sci. 2023 Feb 17;24(4):4066
SW620 cells
10 and 20 μM
48 hours
Chrysin inhibits EMT and malignant progression of colorectal cancer cells by inhibiting the COMP/TAGLN complex.
Theranostics. 2020 Jul 9;10(19):8790-8806.
HCT-8 cells
10 and 20 μM
48 hours
Chrysin inhibits EMT and malignant progression of colorectal cancer cells by inhibiting the COMP/TAGLN complex.
Theranostics. 2020 Jul 9;10(19):8790-8806.
HCT116 cells
10 and 20 μM
48 hours
Chrysin inhibits EMT and malignant progression of colorectal cancer cells by inhibiting the COMP/TAGLN complex.
Theranostics. 2020 Jul 9;10(19):8790-8806.
SK-BR-3 cells
20 μM
Induce cell cycle arrest and inhibit proliferation
Signal Transduct Target Ther. 2023 Dec 18;8(1):463.
BT-474 cells
20 μM
Inhibit proliferation of HER2-positive breast cancer cells
Signal Transduct Target Ther. 2023 Dec 18;8(1):463.
Assessed cytotoxicity, results showed no cytotoxicity at concentrations below 50 μmol/L
Protein Cell. 2013 Jul;4(7):539-47.
Bel-7402
30μM
8h
Inhibited glycolysis and induced apoptosis
J Exp Clin Cancer Res. 2017 Mar 20;36(1):44.
SMMC-7721
30μM
8h
Inhibited glycolysis and induced apoptosis
J Exp Clin Cancer Res. 2017 Mar 20;36(1):44.
HCC-LM3
30μM
8h
Inhibited glycolysis and induced apoptosis
J Exp Clin Cancer Res. 2017 Mar 20;36(1):44.
RAW264.7 monocyte/macrophages
50μM
6 hours
To investigate the inhibitory effect of Chrysin on LPS and IFNγ-induced iNOS gene expression. Results showed that Chrysin significantly attenuated the expression of iNOS gene.
Environ Health Perspect. 2003 Dec;111(16):1877-82.
MCF-7 cells
1 or 10 µM
24 hours
Assess AhR agonist activity, 10 µM Chrysin induced a 5.5-fold increase in luciferase activity
Environ Health Perspect. 2003 Dec;111(16):1877-82.
Hepa-1 cells
1 or 10 µM
24 hours
Assess AhR agonist activity, 10 µM Chrysin induced a 14-fold increase in luciferase activity
Environ Health Perspect. 2003 Dec;111(16):1877-82.
IPSCs-derived neurons
100 μM
20 minutes
Evaluate the inhibitory effect of Chrysin on spontaneous discharges and 4-AP-induced epileptiform activity. Results showed that Chrysin significantly inhibited spontaneous discharges at 100 μM but had no significant effect on 4-AP-induced epileptiform activity.
J Adv Res. 2024 Oct;64:249-262.
Chrysin/白杨素 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6J mice
Experimental autoimmune uveitis (EAU)
Intragastric
25 mg/kg
Once daily from 3 days before immunization to 21 days after immunization
Chrysin significantly reduced the clinical and histopathological scores of EAU, increased the integrity of the blood-retinal barrier, and enhanced the expression of tight junction proteins. Additionally, Chrysin significantly decreased the proportions of Th1, Th17, and CD4+CD3+CD62L+Th0 cells while increasing the proportion of Treg cells. Chrysin also inhibited macrophage infiltration and the expression of inducible nitric oxide synthase in the retina, reduced the expression of interferon-γ, IL-17A, IL-6, IL-1β, and tumor necrosis factor-α, and elevated the levels of transforming growth factor-β. Furthermore, NF-κBp65 expression was downregulated after Chrysin treatment.
Cell Mol Immunol. 2017 Aug;14(8):702-711
BALB/c nude mice
Subcutaneous xenograft model of colorectal cancer
Intraperitoneal injection
20 mg/kg
Once daily until the endpoint of the experiment
Chrysin significantly inhibited the growth and metastasis of colorectal cancer.
Theranostics. 2020 Jul 9;10(19):8790-8806.
BALB/c nude mice
HER2-positive breast cancer xenograft model
Gavage
50 mg/kg
Once daily for 21 days
Inhibit tumor growth
Signal Transduct Target Ther. 2023 Dec 18;8(1):463.
Nude mice
HCC-LM3 xenograft model
Intraperitoneal injection
30 mg/kg
Three times weekly
Inhibited tumor growth and decreased HK-2 expression
J Exp Clin Cancer Res. 2017 Mar 20;36(1):44.
Zebrafish
PTZ-induced seizure model
Incubation
100 μg/mL
20 minutes
Evaluate the inhibitory effect of Chrysin on PTZ-induced seizure activity. Results showed that Chrysin had no significant anti-seizure effect at 100 μg/mL.
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