ADH-1 (0.2 mg/mL) inhibits collagen I-induced alterations in pancreatic cancer cells and significantly suppresses cell motility induced by N-cadherin expression. ADH-1 (0, 0.1, 0.2, 0.5 and 1.0 mg/mL) induces apoptosis in a dose-dependent and N-cadherin-dependent manner [1].
体内研究
ADH-1 (50 mg/kg) markedly inhibits tumor growth and metastasis in a murine model of pancreatic cancer. Additionally, ADH-1 prevents tumor cell invasion and metastasis in an orthotopic model of pancreatic cancer using N-cadherin-overexpressing BxPC-3 cells [1].
ADH-1, at the doses tested, does not exhibit antiangiogenic activity in a rat aortic ring assay or demonstrate antitumor efficacy in a PC3 subcutaneous xenograft tumor model [2].
In A375 xenografts, but not in DM443 xenografts, ADH-1 treatment leads to an increase in phosphorylation of AKT at serine 473. Additionally, ADH-1 slightly reduces N-cadherin expression in both xenografts [3].
体外研究
ADH-1 (0.2 mg/mL) inhibits collagen I-induced alterations in pancreatic cancer cells and significantly suppresses cell motility induced by N-cadherin expression. ADH-1 (0, 0.1, 0.2, 0.5 and 1.0 mg/mL) induces apoptosis in a dose-dependent and N-cadherin-dependent manner [1].
ADH-1 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Fibroblasts (HCA2-hTERT)
1 mg/ml
12, 24 or 48 h
ADH-1 had no effect on the survival of fibroblasts
PLoS One. 2012;7(2):e31206
Human airway smooth muscle cells (HASM)
250 μg/ml
10 min
To evaluate the effect of ADH-1 on F-actin remodeling in HASM cells, results showed that ADH-1 significantly reduced histamine-induced F-actin formation.
Sci Adv. 2024 Nov 29;10(48):eadp8872
Human airway smooth muscle cells (HASM)
500 μg/ml
24 h
To evaluate the effect of ADH-1 on HASM cell contractility, results showed that ADH-1 significantly inhibited histamine-induced cell contraction.
Sci Adv. 2024 Nov 29;10(48):eadp8872
Human umbilical vein endothelial cells (HUVEC)
1mg/mL
1 hour
To evaluate the effect of ADH-1 on HUVEC cell permeability. Results showed that ADH-1 treatment approximately doubled the permeability of HUVEC cells to 40kDa FITC-dextran.
Ann Surg. 2015 Feb;261(2):368-77
MDA-MB-468 cells
40 µM
48 h
To evaluate the effect of ADH-1 on the proliferation and attachment of MDA-MB-468 cells. Results showed that ADH-1 significantly inhibited the attachment of MDA-MB-468 cells.
J Cell Mol Med. 2022 Apr;26(8):2392-2403
DU145 cells
40 µM
48 h
To evaluate the effect of ADH-1 on the proliferation and attachment of DU145 cells. Results showed that ADH-1 had a weaker inhibitory effect on the attachment of DU145 cells.
J Cell Mol Med. 2022 Apr;26(8):2392-2403
PC3 cells
40 µM
48 h
To evaluate the effect of ADH-1 on the proliferation and attachment of PC3 cells. Results showed that ADH-1 significantly inhibited the proliferation and attachment of PC3 cells.
J Cell Mol Med. 2022 Apr;26(8):2392-2403
SH-SY5Y cells
0.5 mg/ml
24 h
Treatment with ADH-1 resulted in significant decrease of N-cadherin immunoreactivity at cell-cell contact sites
J Biol Chem. 2011 Mar 4;286(9):7619-28
Epithelial cells (MCF-7)
1 mg/ml
12, 24 or 48 h
ADH-1 had no effect on the survival of N-cadherin negative epithelial cells
PLoS One. 2012;7(2):e31206
Neuroblastoma cell lines (CLB-GA, IMR32, SK-N-SH)
0.25; 0.50 or 1 mg/ml
12, 24 or 48 h
ADH-1 strongly inhibited neuroblastoma cell proliferation in vitro by inducing apoptosis
PLoS One. 2012;7(2):e31206
ADH-1 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice (Balb/c)
Allergic airway inflammation model
Intraperitoneal injection
200 mg/kg
Single dose
To evaluate the effect of ADH-1 on airway hyperresponsiveness in an allergic airway inflammation model, results showed that ADH-1 significantly reduced MCh-induced airway resistance.
Sci Adv. 2024 Nov 29;10(48):eadp8872
NOD SCID gamma (NSG) mice
PC3 tumor model
Intraperitoneal injection
200 mg/kg
5 days a week
To evaluate the adjuvant effect of ADH-1 on TIL-related therapy
J Immunother Cancer. 2021 Mar;9(3):e002138.
Nude rats
DM443 and A375 melanoma xenograft models
Intraperitoneal injection
100 mg/kg
Single injection
To evaluate the effect of ADH-1 on melanoma xenograft models. Results showed that ADH-1 increased vascular permeability but did not significantly alter tumor interstitial fluid pressure. Additionally, ADH-1 increased LPAM delivery but had no significant effect on TMZ delivery.
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