Daidzin is also a potent and selective inhibitor of human mitochondrial aldehyde dehydrogenase (ALDH-2) with IC50 of 80 nM[3]. Daidzin is also an isoflavone that has anti-oxidant, anti-carcinogenic, and anti-atherosclerotic activities[4]. Daidzin possess tyrosyl radical scavenging activity and thereby decreases the oxidative stress. Treatment with daidzin protects the cornea in dry eye rat model by suppression inflammation and oxidative stress[5]. ITG extract and its active component Daidzin effectively improved CYP-induced oxidative stress, inflammation, and fibrosis through inhibiting the MMP-8, TIMP-1, and oxidative stress[6]. Daidzin treatment (150 mg/kg per day i.p. for 6 days) significantly suppresses (> 70%) hamster ethanol intake but does not affect overall acetaldehyde metabolism. In vitro, daidzin suppresses hamster liver mitochondria-catalyzed acetaldehyde oxidation very potently with an IC50 value of 0.4 mM, which is substantially lower than the daidzin concentration (70 mM) found in the liver mitochondria of daidzin-treated hamsters[7]. Daidzin inhibits ALDH-I competitively with respect to formaldehyde with a Ki of 40 nM, and uncompetitively with respect to the coenzyme NAD+[8].
Daidzin/大豆苷 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Hamster liver mitochondria
0.4 μM
10 min
To study the inhibitory effect of Daidzin on mitochondria-catalyzed acetaldehyde oxidation, results showed Daidzin significantly inhibited acetaldehyde oxidation with an IC50 of 0.4 μM.
Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8990-3
neonatal rat cardiomyocytes
20 µM
48 h
To investigate the effect of Daidzin on mitochondrial membrane potential in cardiomyocytes under high glucose conditions. Results showed that high glucose treatment significantly decreased mitochondrial membrane potential, and Daidzin further exacerbated this effect.
Mol Med. 2011 Mar-Apr;17(3-4):172-9
Neuro2A cells
20 or 40 µM
12 h
To study the effect of ALDH2 inhibition on alcohol-induced neuronal damage, results showed that Daidzin pretreatment exacerbated ethanol-induced cell viability reduction and oxidative stress-related PTMs.
Cells. 2024 May 28;13(11):927
T84 human colon cells
10 μM
12 h
Pretreatment with Daidzin exacerbated ethanol-induced cell permeability and reduced TJ/AJ proteins in T84 human colon cells. These changes were reversed by Alda-1, an ALDH2 activator.
Redox Biol. 2023 Feb;59:102577.
Daidzin/大豆苷 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Syrian golden hamsters
Free-choice ethanol intake model
Intraperitoneal injection
150 mg/kg/day
Once daily for 6 days
To study the effect of Daidzin on hamster ethanol intake and its impact on acetaldehyde metabolism, results showed Daidzin significantly suppressed ethanol intake (70%) without affecting acetaldehyde metabolism.
Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8990-3
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