There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
The importance of the Ca2+-ATPases of the surface and endoplasmic reticulum membranes in regulating the intracellular Ca2+ concentrations in living cells has been recognized as a result of the development of biophysical techniques and finding of useful pharmacological tool drugs[1]. BHQ is a potent and selective inhibitor of the sarco-endoplasmic reticulum Ca2+-ATPase (SERCA). In rat tail artery myocytes, BHQ reduced ICa(L) in a concentration- and voltage-dependent manner with an IC50 of 66.7 μM[2]. In rat basophilic leukaemia (RBL-2H3) cells under whole cell voltage clamp, a maximal blockade of inward current was obtained within 6 min after perfusion with 10 μM BHQ which caused depolarization of the cell which would affect Ca2+ influx[3].
2,5-Di-tert-butylhydroquinone 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Eremosphaera viridis
10 µM
2.9 ± 1.2 minutes
Inhibited the ER Ca2+-ATPase, thereby preventing Sr2+-induced repetitive [Ca2+]cy spikes
Plant Physiol. 1998 Jun;117(2):545-57.
HL60 cells
0.31 µM, 0.63 µM, 1.25 µM
48 hours
Evaluate the cytotoxicity of BHQ on HL60 cells, showing lower cytotoxicity for the parental cell line
Mol Pharm. 2013 Nov 4;10(11):4358-66.
HL60/MX2 cells
0.31 µM, 0.63 µM, 1.25 µM
48 hours
Evaluate the cytotoxicity of BHQ on HL60/MX2 cells, showing selective cytotoxicity for the resistant cell line (7.7 fold)
Mol Pharm. 2013 Nov 4;10(11):4358-66.
Mouse cardiomyocytes
10 µM
Evaluate the effect of strong SERCA2a inhibition on Ca2+ alternans, showing significant reduction in Ca2+ transient amplitude and suppression of alternans
J Biol Chem. 2018 Aug 31;293(35):13650-13661.
2,5-Di-tert-butylhydroquinone 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Caenorhabditis elegans
Ret-1(syb4955) mutant
Exposure in culture medium
100 μM
Single dose, 4-hour exposure
Inhibition of ER/SR Ca2+ reuptake rescued key phenotypic differences in the mutant
Brain. 2024 Jul 5;147(7):2334-2343
Mice
RyR2 WT and mutant Mice models
Perfusion
3 μM and 10 μM
5-14 Hz stimulation frequency
Evaluate the effect of tBHQ on Ca2+ alternans, showing minor impact with 3 μM tBHQ and significant suppression with 10 μM tBHQ
J Biol Chem. 2018 Aug 31;293(35):13650-13661.
Mice
RyR2 WT and RyR2 R4496C mutant mice
Perfusion
3 μM and 10 μM
Electrical stimulation at various frequencies (5-14 Hz)
To evaluate the effect of tBHQ on Ca2+ alternans. 3 μM tBHQ had little impact on Ca2+ alternans, whereas 10 μM tBHQ markedly reduced the amplitude of Ca2+ transients and suppressed Ca2+ alternans.
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