Tetraethylammonium chloride is recognized as a non-selective blocker of potassium channels and is also an effective substrate for the organic cation transporter (OCTN1). It exhibits notable antitumor properties[1].[2].
体内研究
Further research indicates that Tetraethylammonium at concentrations of 1 mM, 3 mM, and 5 mM significantly enhances the amplitude and frequency of contractility in the colon and rectum of rats, both in longitudinal and circular directions. It was also found that Tetraethylammonium at 5 mM and 15 mM does not affect the histology of the colon and rectum when administered locally into the colon lumen from the anus for 10 days[2].
体外研究
In studies involving C6 and 9L glioma cells, treatment with Tetraethylammonium chloride ranging from 0.2 to 60 mM over 24 to 72 hours inhibits cell proliferation in a dose- and time-dependent manner[1].
At a concentration of 40 mM applied over 24 to 72 hours, this treatment significantly increases apoptosis in these cells[1].
Additionally, the same treatment at 40 mM for 12 to 48 hours significantly elevates the Bax/Bcl-2 protein ratio, indicating an increase in pro-apoptotic signaling in a time-dependent manner[1].
The generation of intracellular reactive oxygen species (ROS) is increased in C6 and 9L cells with the addition of 20 and 40 mM Tetraethylammonium[1].
Tetraethylammonium chloride/四乙基氯化铵 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Rat brain synaptic membranes
6 mM
100 minutes
Tetraethylammonium chloride (TEA) at 6 mM concentration inhibited the binding of 125I-apamin to the solubilized receptor, indicating its role as a potassium channel blocker.
J Neurosci. 1987 Feb;7(2):565-70
BY-2 cells
10 mM
12 hours
To study the inhibitory effect of TEA+ on potassium channels in BY-2 cells, results showed that TEA+ significantly inhibited K+ influx
J Nanobiotechnology. 2020 Jan 28;18(1):21
Human colon circular muscle strips
10 mM
30 minutes
To study the effect of TRPM8 activation on human colon smooth muscle contractions, results showed TEA significantly reduced the inhibitory effect of DIPA 1-8
Int J Mol Sci. 2020 Jul 29;21(15):5403
HeLa cells
20 mM
36 hours
To investigate the inhibitory effect of TEA on HeLa cell proliferation and its ability to induce apoptosis. Results showed that TEA reduced HeLa cell viability dose-dependently and significantly increased the apoptosis rate.
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