Stevioside is obtained from leaves of Stevia rebaudiana. Stevioside is an important source of biochemically active substances with proven anti-diabetic effect. Stevioside solution prevented significant increase in glycaemia in oral glucose tolerance test (9.22 ± 1.13 to 9.85 ± 1.32 mmol/l, P < 0.05), and not in adrenaline test[3]. Stevioside inhibited the mRNA and protein expression of TNF-α, IL-6 and IL-1β dose-dependently in S.aureus-stimulated MMECs. Stevioside suppressed the S.aureus-induced expression of TLR2 and proteins of the NF-κB and MAPK pathways as well as apoptosis[4].
Stevioside/甜菊糖 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HepG2 cells
0, 12.5, 25, 50, 100 μM
24 hours
SS treatment augmented LC3B-II/LC3B-I levels and reduced SQSTM1 (p62) levels in a dose-dependent manner.
Antioxidants (Basel). 2022 Dec 19;11(12):2496.
Mouse chondrocytes
0, 10, 20, 40 μM
24 hours
STE significantly inhibited IL-1β-induced expression of iNOS and Cox-2, reduced the generation of MMP-13 and ADAMTS-4, and decreased the degradation of Aggrecan and Collagen II.
J Orthop Translat. 2022 Nov 15;38:190-202.
Mouse chondrocytes
0, 10, 20, 40 μM
24 hours
To evaluate the effect of Stevioside on IL-1β-induced inflammatory response. Results showed that Stevioside significantly inhibited the expression of iNOS and COX-2, reduced the production of MMP-13 and ADAMTS-4, and protected the degradation of cartilage matrix components Aggrecan and Collagen II.
J Orthop Translat. 2022 Nov 15;38:190-202.
3T3-L1 preadipocytes
100-200 μM
7 days
To investigate the effect of stevioside on lipid accumulation in 3T3-L1 adipocytes. Results showed that stevioside dose-dependently reduced intracellular lipid accumulation.
Cells. 2022 Mar 23;11(7):1076.
Human hair follicle dermal papilla cells (HDPs)
0, 0.2, 1, 5, 10, 50 µM
24 and 48 hours
Stevioside alone did not significantly affect the proliferation of human hair follicle dermal papilla cells. At 48 h, stevioside showed 3-5% cytotoxicity in human HDP cells at 10 µM concentration.
Pharmaceutics. 2020 Dec 18;12(12):1234.
Human keratinocytes (HaCaT)
0, 0.2, 1, 5, 10, 50 µM
24 and 48 hours
Stevioside alone promoted the proliferation of human keratinocytes. Compared to the control (CTR), the proliferation of HaCaT cells was increased by 15% after 48 h of treatment with 10 µM stevioside.
Pharmaceutics. 2020 Dec 18;12(12):1234.
Stevioside/甜菊糖 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Db/db mice
Non-alcoholic fatty liver disease model
Oral
40 mg/kg/day
Once daily for 3 weeks
S and SS reduced the body and liver weight and levels of serum triglyceride, total cholesterol, and hepatic lipogenic proteins. In addition, S and SS increased the levels of fatty acid oxidase, peroxisome proliferator-activated receptor alpha (PPARα), and microtubule-associated protein light chain 3 B but decreased that of sequestosome 1 (p62) in the liver of db/db mice.
Antioxidants (Basel). 2022 Dec 19;11(12):2496.
Mice
Trpm5-lacZ+/− mice
Oral
0.5 mM
Enhanced Trpm5 activity, promoting IL-25 release and hyperplasia in tuft cells
Proc Natl Acad Sci U S A. 2019 Mar 19;116(12):5564-5569
C57BL/6 male mice
OA model induced by destabilization of the medial meniscus (DMM)
Oral
50 mg/kg/day
Twice a day for 8 weeks
To evaluate the protective effect of Stevioside on DMM-induced osteoarthritis. Results showed that Stevioside significantly attenuated cartilage degeneration and reduced OARIS scores.
J Orthop Translat. 2022 Nov 15;38:190-202.
Db/db mice
Obesity and diabetes model
Oral gavage
40 mg/kg/day
Once daily for 3 weeks
To investigate the effect of stevioside on epididymal adipose tissues in db/db mice. Results showed that stevioside reduced adipocyte size and improved glucose tolerance and insulin resistance.
United States, Massachusetts
... 展开 >>
Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University
Boston, Massachusetts, United States, 02111 收起 <<
United States, Massachusetts
... 展开 >>
Boston Children's Hospital
Not yet recruiting
Boston, Massachusetts, United States, 02115
Contact: Kimberly I Mills, MD 617-355-7866 kimberly.mills@cardio.chboston.org
Contact: Ben D Albert, MD 617-355-7866 ben.albert@childrens.harvard.edu 收起 <<
Denmark
... 展开 >> Aarhus University Hospital, Dept of Clinical medicine
Recruiting
Aarhus C, Denmark, 8000
Contact: Per B Jeppesen, Prof, PhD +45-28151877 per.bendix.jeppesen@ki.au.dk
Principal Investigator: Per B Jeppesen, Prof, PhD
Sub-Investigator: Søren Larvsen, Prof-Sport 收起 <<
Spain
... 展开 >> Basque Public Health Service-Osakidetza (Lakuabizkarra primary care center and Txagorritxu hospital)
Vitoria-Gasteiz, Alava, Spain, 01009 收起 <<
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