Sarsasapogenin, a natural product isolated and purified from the rhizomes of Anemarrhena asphodeloides Bunge with anti-inflammatory, anti-diabetes,antidepressant, antitumor and protective effects, against glutamate-induced neurotoxicity in the cultured cortical neurons in rats, can improve memory by elevating the low muscarinic acetylcholine receptor density in brains of memory-deficit rat models, and effectively promote the proliferation, differentiation and mineralization of osteoblasts cultured in vitro, also inhibit the generation of osteoclasts from marrow cells.
Sarsasapogenin/菝葜皂苷元 细胞实验
Cell Line
Concentration
Treated Time
Description
References
MDCK-MDR1 cells
1.2 µM
120 minutes
To investigate the interaction between SSG and P-gp, results showed that transmembrane transport of SSG can be regarded as passive transport.
Molecules. 2022 Dec 5;27(23):8556
BV2 cells
0.1–100 µM
24 hours
Assess the effect of SA on BV2 cell viability, showing that concentrations of 0.1–100 μM SA had no significant effect on cell viability.
Heliyon. 2024 Jan 26;10(3):e25145
Bone marrow-derived macrophages (BMM)
1, 2, 4, 8 µM
48 or 96 hours
To assess the cytotoxicity of sarsasapogenin on BMMs, results showed no cytotoxicity at concentrations below 4 μM.
Drug Des Devel Ther. 2020 Aug 24;14:3435-3447
Bone marrow-derived macrophages (BMM)
0, 1, 2, 4 µM
6 days
To evaluate the effect of sarsasapogenin on RANKL-induced osteoclast formation, results showed significant reduction in TRAP-positive osteoclast area and number.
Drug Des Devel Ther. 2020 Aug 24;14:3435-3447
Melan-a cells
1-10 µM
72 hours
SAR significantly increased melanin content in melan-a cells and induced melanogenesis by stimulating tyrosinase and MITF protein expression.
Biomol Ther (Seoul). 2012 May;20(3):340-5
Huh7 cells
20 µM
9 hours
To evaluate the antiviral activity of Sarsasapogenin against CHIKV. Results showed that Sarsasapogenin significantly reduced the production of infectious virus particles, with a 76.4% reduction compared to the EtOH control.
Sci Rep. 2020 Apr 14;10(1):6364
Sarsasapogenin/菝葜皂苷元 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Rats
Sprague-Dawley rats
Oral
100 mg/kg
Single dose
To study the pharmacokinetic properties of SAR in rats, showing a relatively long terminal elimination phase half-life (>17 h)
Acta Pharmacol Sin. 2010 Aug;31(8):984-9
C57/BL6 male mice
LPS-induced calvarial osteolysis model
Intragastric administration
5 mg/kg and 10 mg/kg
Administered every other day for 7 days
To evaluate the protective effect of sarsasapogenin on LPS-induced bone loss, results showed sarsasapogenin significantly inhibited bone loss.
Sci Rep. 2021 Jan 22;11(1):2074
C57/BL6 male mice
LPS-induced calvarial osteolysis model
Intragastric administration
5 mg/kg and 10 mg/kg
Administered every other day for 7 days
To evaluate the protective effect of sarsasapogenin on LPS-induced bone loss, results showed sarsasapogenin significantly inhibited bone loss.
Drug Des Devel Ther. 2020 Aug 24;14:3435-3447
Wistar rats
Spinal cord injury model
Intragastric administration
5, 10, and 20 mg/kg
Once daily until sacrifice
Evaluate the effect of SA on motor function recovery in rats with spinal cord injury, showing that 10 and 20 mg/kg SA significantly improved motor function.
Heliyon. 2024 Jan 26;10(3):e25145
ICR mice
Aβ1-42 intracerebroventricular injection model
Oral
6 mg/kg and 30 mg/kg
Once daily for 7 days
To evaluate the effect of AA13 on learning and memory functions in Aβ1-42-injected mice. Results showed that AA13 significantly improved Aβ1-42-induced learning and memory impairments.
搜索
