Jaceosidin is a flavonoid isolated from Artemisia vestita that has been reported to possess anti-tumor and anti-proliferative activities in many cancer cells. Jaceosidin induced apoptosis in multiple human renal carcinoma cells (Caki, ACHN, A498, and 786-O), lung cancer cells (A549) and glioma cells (U251MG). Treatment with jaceosidin also caused loss of mitochondrial membrane potential (MMP) and Bax activation, which led to the release of cytochrome c into the cytosol[3]. Jaceosidin is a more potent inhibitor of cell growth than cisplatin in human endometrial cancer cells. In contrast, jaceosidin-induced cytotoxicity in normal endometrial cells was lower than that observed for cisplatin. Jaceosidin induced G2/M phase cell cycle arrest and modulated the levels of cyclin B and p-Cdc2 in Hec1A cells. Jaceosidin shows cytostatic activity to HES and HESC cells with IC50s of 52.68 and 55.10 μM, and is less cytotocxic on Hec1 A and KLE (IC50, 70.54, 147.14 μM, respectively), after treatment for 48 h[4]. Jaceosidin could improve ER (endoplasmic reticulum) stress and attenuate insulin resistance via SERCA2b (sarco-endoplasmic reticulum Ca2+-ATPase 2b) upregulation in mice skeletal muscles[5]. Mice were orally administrated with Jaceosidin (15, 30 and 60 mg/kg) after LPS challenge. 24 h after LPS (lipopolysaccharide) challenge, Jaceosidin could significantly decrease the lung wet-to-dry weight (W/D) ratio and the protein concentration in bronchoalveolar lavage fluid (BALF) [6].
Jaceosidin/棕矢车菊素 细胞实验
Cell Line
Concentration
Treated Time
Description
References
12 types of gastric cancer cells (including AGS, KATO-3, etc.)
20, 40, 60, 80, 100 μM
6, 12, 18, 24, 30 hours
JAC showed killing effects on various GC cells with IC50 values ranging from 38.65 to 90.46 μM
Redox Rep. 2024 Dec;29(1):2313366
GES-1 normal gastric cells
39 μM
3, 6, 12, 24 hours
No increase in apoptosis observed
Redox Rep. 2024 Dec;29(1):2313366
AGS gastric cancer cells
39 μM
3, 6, 12, 24 hours
Induced apoptosis in AGS cells via the mitochondrial pathway by upregulating Cyto-c, Bad, cle-caspase-3, and cle-PARP expressions and downregulating Bcl-2 expression
Redox Rep. 2024 Dec;29(1):2313366
mouse articular chondrocytes
10, 20, 40, 80 μmol/L
12 hours
To evaluate the inhibitory effect of Jaceosidin on IL-1β-induced expression of MMP3, MMP13, ADAMTS4, and ADAMTS5. Results showed that Jaceosidin decreased the expression of these proteins in a concentration-dependent manner.
J Cell Mol Med. 2020 Jul;24(14):8126-8137
H1975 cells
0, 6, 12, 24 μM
12, 24, 36 hours
Evaluate the inhibitory effect of Jaceosidin on the migration of H1975 cells, the results showed that Jaceosidin significantly inhibited the migration of H1975 cells.
Heliyon. 2023 May 11;9(5):e16158
A549 cells
0, 6, 12, 24 μM
12, 24, 36 hours
Evaluate the inhibitory effect of Jaceosidin on the migration of A549 cells, the results showed that Jaceosidin significantly inhibited the migration of A549 cells.
Heliyon. 2023 May 11;9(5):e16158
H1975 cells
9.19 ±1.90 μM (IC50)
72 hours
Evaluate the inhibitory effect of Jaceosidin on the proliferation of H1975 cells, the results showed that Jaceosidin significantly inhibited the proliferation of H1975 cells.
Heliyon. 2023 May 11;9(5):e16158
A549 cells
12.71 ±0.91 μM (IC50)
72 hours
Evaluate the inhibitory effect of Jaceosidin on the proliferation of A549 cells, the results showed that Jaceosidin significantly inhibited the proliferation of A549 cells.
Heliyon. 2023 May 11;9(5):e16158
oral squamous cell carcinoma YD-8 cells
10-50 μM
24 hours
JAC had a minor effect on the survival of YD-8 cells.
Am J Cancer Res. 2021 Oct 15;11(10):4919-4930
Osteosarcoma MG63 cells
10-50 μM
24 hours
JAC had a minor effect on the survival of MG63 cells.
Am J Cancer Res. 2021 Oct 15;11(10):4919-4930
human GBM T98G cells
10-50 μM
24 hours
JAC significantly reduced the survival of T98G cells, induced apoptotic cell death, altered morphological and molecular phenotypes, increased DNA fragmentation, activated cleavage of caspase-3 and PARP, and downregulated the expression of survivin and Bcl-2.
Am J Cancer Res. 2021 Oct 15;11(10):4919-4930
HaCaT cells
12.5–100 μM
24 hours
Jaceosidin at concentrations of 12.5–100 μM did not affect the proliferation of HaCaT normal epithelial keratinocytes, indicating selective cytotoxicity towards cancer cells.
Evid Based Complement Alternat Med. 2018 May 13;2018:5765047
Ca9.22 cells
97.5 μM
24 hours
Jaceosidin inhibited Ca9.22 cell proliferation with an IC50 of 97.5 μM, causing morphological changes and cell cycle arrest at the sub-G1 phase.
Evid Based Complement Alternat Med. 2018 May 13;2018:5765047
HSC-3 cells
82.1 μM
24 hours
Jaceosidin inhibited HSC-3 cell proliferation with an IC50 of 82.1 μM, causing morphological changes and cell cycle arrest at the sub-G1 phase.
Evid Based Complement Alternat Med. 2018 May 13;2018:5765047
Jaceosidin/棕矢车菊素 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6 mice
DMM-induced osteoarthritis model
Oral
50 mg/kg
Every other day for 10 weeks
To evaluate the protective effect of Jaceosidin on DMM-induced cartilage destruction. Results showed that Jaceosidin significantly inhibited cartilage destruction, reduced OARSI scores, and subchondral bone plate thickness.
J Cell Mol Med. 2020 Jul;24(14):8126-8137
Balb/c nude mice
A549 cell xenograft model
Intraperitoneal injection
25 mg/kg and 50 mg/kg
7 consecutive days
Evaluate the inhibitory effect of Jaceosidin on tumor growth in A549 cell xenograft model, the results showed that Jaceosidin significantly inhibited tumor growth.
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