Ginkgetin is a natural biflavonoid isolated from leaves of Ginkgo biloba L and effects of anti-inflammation and anticancer have been reported.
Ginkgetin/银杏双黄酮 细胞实验
Cell Line
Concentration
Treated Time
Description
References
IPEC-J2 cells
0, 6.25, 12.5, 25, 50, 100, 200 μM
24 hours
Evaluate the cytotoxicity of Ginkgetin on IPEC-J2 cells. Results showed that Ginkgetin at 50 μM or lower concentrations had no cytotoxic effect, while cytotoxicity appeared at 100 μM and 200 μM.
Int J Mol Sci. 2022 Aug 8;23(15):8809
Caco-2 cells
0, 6.25, 12.5, 25, 50, 100, 200 μM
24 hours
Evaluate the cytotoxicity of Ginkgetin on Caco-2 cells. Results showed that Ginkgetin at 50 μM or lower concentrations had no cytotoxic effect, while cytotoxicity appeared at 100 μM and 200 μM.
Int J Mol Sci. 2022 Aug 8;23(15):8809
human umbilical vein endothelial cells (HUVECs)
0.1, 0.3, 1 μM
48 hours
inhibited VEGF-induced endothelial cell proliferation, migration, and tube formation
Cancers (Basel). 2019 Nov 20;11(12):1828
4T1
5.7-11.5 µmol/L
48-72 hours
Evaluate the anti-proliferative activity of Ginkgetin, results showed that Gk significantly increased the proportion of apoptotic cells in a dose-dependent manner.
Chin Med J (Engl). 2024 Oct 5;137(19):2387-2389
SUM-159
5.7-11.5 µmol/L
48-72 hours
Evaluate the anti-proliferative activity of Ginkgetin, results showed that Gk significantly increased the proportion of apoptotic cells in a dose-dependent manner.
Chin Med J (Engl). 2024 Oct 5;137(19):2387-2389
MCF-7
5.7-11.5 µmol/L
48-72 hours
Evaluate the anti-proliferative activity of Ginkgetin, results showed that Gk significantly increased the proportion of apoptotic cells in a dose-dependent manner.
Chin Med J (Engl). 2024 Oct 5;137(19):2387-2389
MDA-MB-436
5.7-11.5 µmol/L
48-72 hours
Evaluate the anti-proliferative activity of Ginkgetin, results showed that Gk significantly increased the proportion of apoptotic cells in a dose-dependent manner.
Chin Med J (Engl). 2024 Oct 5;137(19):2387-2389
MDA-MB-453
5.7-11.5 µmol/L
48-72 hours
Evaluate the anti-proliferative activity of Ginkgetin, results showed that Gk significantly increased the proportion of apoptotic cells in a dose-dependent manner.
Chin Med J (Engl). 2024 Oct 5;137(19):2387-2389
MDA-MB-231
10 µmol/L
48-72 hours
Evaluate the anti-proliferative activity of Ginkgetin, results showed that Gk significantly increased the proportion of apoptotic cells in a dose-dependent manner and inhibited cell migration.
Chin Med J (Engl). 2024 Oct 5;137(19):2387-2389
THP-1-derived macrophages (THP-1 mφs)
10 μM
6 hours
Ginkgetin significantly suppressed the upregulation of IFNB1 and TNF induced by cGAS or STING agonist, but not by RIG-I/MDA5 or TLR4 agonist, indicating selective inhibition of the cGAS-STING signaling pathway.
Adv Sci (Weinh). 2025 Jan;12(2):e2407222
Mouse embryonic fibroblasts (MEFs)
2 μM
48 hours
Ginkgetin significantly alleviated cellular senescence phenotypes induced by Dox, downregulating the expression of CDKIs (including p16 and p21) and SASP-related genes (such as Il6 and Il1b), and decreasing the number of SA-β-gal positive cells.
Adv Sci (Weinh). 2025 Jan;12(2):e2407222
Ginkgetin/银杏双黄酮 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6J mice
Dox-induced aging mouse model
Intraperitoneal injection
5 mg/kg
Every 2 days for 2 months
Ginkgetin effectively reduced the elevated expression of CDKIs (p16 and p21) and SASP-related cytokines (Il1b and Il6) in multiple organs (kidney, liver, muscle, and spleen), diminished the accumulation of SA-β-Gal positive senescent cells in the kidney, and ameliorated severe inflammation in the kidney and liver of aging mice.
Synergistically enhanced the anti-tumor effect of 5-FU by inhibiting angiogenesis
Cancers (Basel). 2019 Nov 20;11(12):1828
SCID mice
MDA-MB-231 xenograft model
Intraperitoneal injection
30 mg/kg
Evaluate the anti-tumor activity of Ginkgetin in vivo, results showed that Gk significantly suppressed tumor growth without significantly affecting body weight.
Chin Med J (Engl). 2024 Oct 5;137(19):2387-2389
C57BL/6 mice
LPS-induced acute kidney injury model
Intraperitoneal injection
200 mg/kg
Single dose, lasted for 24 hours
GA attenuated LPS-induced inflammatory response and renal injury by activating SIRT1 and inhibiting the NF-κB signaling pathway
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