Daphnoretin, a natural product isolated and purified from the roots of Wikstroemia indica (Linn.) C. A. Mey., has strong antiviral activity, mainly on the later phase of the replication cycle. Daphnoretin also has anticancer effects on leukemia, osteosarcoma and uterine cervix cancer cells, might through promoting apoptosis in a mitochondria-mediated way.
Daphnoretin 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HEB cells
0, 10, 20, 40, 80, 100 μM
24 and 48 h
To evaluate the effect of Daphnoretin on normal brain glial cells. Results showed that Daphnoretin had low toxicity on HEB cells.
J Cancer. 2024 Sep 9;15(17):5810-5827.
U251 cells
0, 10, 20, 40, 80, 100 μM
24 and 48 h
To evaluate the inhibitory effect of Daphnoretin on GBM cell proliferation. Results showed that Daphnoretin significantly inhibited GBM cell proliferation in a concentration-dependent manner.
J Cancer. 2024 Sep 9;15(17):5810-5827.
U87 cells
0, 10, 20, 40, 80, 100 μM
24 and 48 h
To evaluate the inhibitory effect of Daphnoretin on GBM cell proliferation. Results showed that Daphnoretin significantly inhibited GBM cell proliferation in a concentration-dependent manner.
J Cancer. 2024 Sep 9;15(17):5810-5827.
human osteosarcoma (HOS) cells
1-4 μM
48 h
Flow cytometric analysis showed Daphnoretin arrested the cell cycle in the G2/M phase in a concentration-dependent manner.
Molecules. 2012 Jan 9;17(1):598-612.
human osteosarcoma (HOS) cells
1-4 μM
48 h
Apoptosis was detected using Annexin V-FITC/PI apoptosis detection kit, showing Daphnoretin induced apoptosis in a concentration-dependent manner.
Molecules. 2012 Jan 9;17(1):598-612.
human osteosarcoma (HOS) cells
3.89 μM
72 h
To evaluate the antiproliferative activity of Daphnoretin on HOS cells, showing significant inhibitory effects.
Molecules. 2012 Jan 9;17(1):598-612.
HEL cells
0.25 µM and 1.00 µM
24, 48, and 72 h
Daphnoretin inhibited the growth of HEL cells in a dose- and time-dependent manner.
Cells. 2022 Oct 16;11(20):3252.
K562 cells
0.25 µM and 1.00 µM
24, 48, and 72 h
Daphnoretin inhibited the growth of K562 cells in a dose- and time-dependent manner.
Cells. 2022 Oct 16;11(20):3252.
Daphnoretin 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6 mice
GBM xenograft model
Subcutaneous injection
20 mg/kg,40 mg/kg
Daily administration for 14 days
To evaluate the inhibitory effect of Daphnoretin on GBM tumor growth in vivo. Results showed that Daphnoretin significantly inhibited tumor growth with no significant toxicity.
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